Promotion of tendon growth into implant through pore-size design of a Ti-6Al-4 V porous scaffold prepared by 3D printing

Zheng, Yuhao; Han, Qing; Li, Dongdong; Sheng, Fan; Song, Zhiming; Wang, Jincheng

doi:10.1016/j.matdes.2020.109219

Cited by 27 publications

(13 citation statements)

References 39 publications

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“…The size of our pores was determined according to our previous studies and by reports made by others. Zheng et al reported that macro-porous scaffolds of about 100 and 600 μm allowed better integration with the host bone tissue and also for subsequent vascularization and bone distribution [ 53 ]. Quantitative results were computed to further confirm the percentage of bone growth.…”

Section: Resultsmentioning

confidence: 99%

Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration

et al. 2021

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Periodontal disease is a chronic disease that can lead to lose teeth and even tooth loss if left untreated. Osteoporosis and periodontal disease share similar characteristics and associated factors. Current regenerative techniques for periodontal diseases are ineffective in restoring complete function and structural integrity of periodontium due to unwanted migration of cells. In this study, we applied the concept of guided tissue regeneration (GTR) and 3D fabricated gingival fibroblast cell-laden collagen/strontium-doped calcium silicate (SrCS) bi-layer scaffold for periodontal regeneration. The results revealed that the bioactive SrCS had a hydroxyapatite formation on its surface after 14 days of immersion and that SrCS could release Sr and Si ions even after 6 months of immersion. In addition, in vitro results showed that the bi-layer scaffold enhanced secretion of FGF-2, BMP-2, and VEGF from human gingival fibroblasts and increased secretion of osteogenic-related proteins ALP, BSP, and OC from WJMSCs. In vivo studies using animal osteoporotic models showed that the 3D-printed cell-laden collagen/SrCS bi-layer scaffold was able to enhance osteoporotic bone regeneration, as seen from the increased Tb.Th and BV/TV ratio and the histological stains. In conclusion, it can be seen that the bi-layer scaffolds enhanced osteogenesis and further showed that guided periodontal regeneration could be achieved using collagen/SrCS scaffolds, thus making it a potential candidate for future clinical applications.

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Section: Resultsmentioning

confidence: 99%

Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration

et al. 2021

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“…Inter-connectivity and pores are important features for bone regenerative scaffolds as pore sizes of between 300–550 μm have been reported to enhance cellular adhesion and subsequent bone regeneration. In a previous study, 3D scaffolds with a pore size between 200 to 500 µm were recommended for bone regeneration because of the high initial cell attachment, reasonable migration, and inhibition of cell aggregation [ 9 ]. The struts were neatly stacked on top of one another and the distance between each strut was similar to our original designs.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, three-dimensional (3D) printing has gained popularity in this field of study due to its capability to personalize and customize scaffolds for large bone defects and wound repair [ 8 , 9 , 10 ]. Furthermore, interconnected nanopores could be fabricated using 3D printing, with nanopores reported as being able to provide a suitable microenvironment for cell proliferation and attachment [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

3D-Printed Ginsenoside Rb1-Loaded Mesoporous Calcium Silicate/Calcium Sulfate Scaffolds for Inflammation Inhibition and Bone Regeneration

et al. 2021

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Bone defects are commonly found in the elderly and athletic population due to systemic diseases such as osteoporosis and trauma. Bone scaffolds have since been developed to enhance bone regeneration by acting as a biological extracellular scaffold for cells. The main advantage of a bone scaffold lies in its ability to provide various degrees of structural support and growth factors for cellular activities. Therefore, we designed a 3D porous scaffold that can not only provide sufficient mechanical properties but also carry drugs and promote cell viability. Ginsenoside Rb1 (GR) is an extract from panax ginseng, which has been used for bone regeneration and repair since ancient Chinese history. In this study, we fabricated scaffolds using various concentrations of GR with mesoporous calcium silicate/calcium sulfate (MSCS) and investigated the scaffold’s physical and chemical characteristic properties. PrestoBlue, F-actin staining, and ELISA were used to demonstrate the effect of the GR-contained MSCS scaffold on cell proliferation, morphology, and expression of the specific osteogenic-related protein of human dental pulp stem cells (hDPSCs). According to our data, hDPSCs cultivated in GR-contained MSCS scaffold had preferable abilities of proliferation and higher expression of the osteogenic-related protein and could effectively inhibit inflammation. Finally, in vivo performance was assessed using histological results that revealed the GR-contained MSCS scaffolds were able to further achieve more effective hard tissue regeneration than has been the case in the past. Taken together, this study demonstrated that a GR-containing MSCS 3D scaffold could be used as a potential alternative for future bone tissue engineering studies and has good potential for clinical use.

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“…Porous titanium alloy-based scaffold, Ti-6Al-4V, was fabricated using 3DP and tested in vivo for possible tendon fixation following prosthetic implantation. Micro-CT and hard tissue staining visualized the increased fibroblasts adhesion to and growth into the 3D-printed scaffolds of 527.15 µm pore size [199].…”

Section: Three-dimensional Printing (3dp) and Bioprintingmentioning

confidence: 99%

Conventional and Recent Trends of Scaffolds Fabrication: A Superior Mode for Tissue Engineering

ElMeligy

2022

Pharmaceutics

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Tissue regeneration is an auto-healing mechanism, initiating immediately following tissue damage to restore normal tissue structure and function. This falls in line with survival instinct being the most dominant instinct for any living organism. Nevertheless, the process is slow and not feasible in all tissues, which led to the emergence of tissue engineering (TE). TE aims at replacing damaged tissues with new ones. To do so, either new tissue is being cultured in vitro and then implanted, or stimulants are implanted into the target site to enhance endogenous tissue formation. Whichever approach is used, a matrix is used to support tissue growth, known as ‘scaffold’. In this review, an overall look at scaffolds fabrication is discussed, starting with design considerations and different biomaterials used. Following, highlights of conventional and advanced fabrication techniques are attentively presented. The future of scaffolds in TE is ever promising, with the likes of nanotechnology being investigated for scaffold integration. The constant evolvement of organoids and biofluidics with the eventual inclusion of organ-on-a-chip in TE has shown a promising prospect of what the technology might lead to. Perhaps the closest technology to market is 4D scaffolds following the successful implementation of 4D printing in other fields.

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Promotion of tendon growth into implant through pore-size design of a Ti-6Al-4 V porous scaffold prepared by 3D printing

Cited by 27 publications

References 39 publications

Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration

Biofabrication of Gingival Fibroblast Cell-Laden Collagen/Strontium-Doped Calcium Silicate 3D-Printed Bi-Layered Scaffold for Osteoporotic Periodontal Regeneration

3D-Printed Ginsenoside Rb1-Loaded Mesoporous Calcium Silicate/Calcium Sulfate Scaffolds for Inflammation Inhibition and Bone Regeneration

Conventional and Recent Trends of Scaffolds Fabrication: A Superior Mode for Tissue Engineering

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