Promotion of Sophora subprosrate polysaccharide on nitric oxide and interleukin-2 production in murine T lymphocytes: implicated Ca2+ and protein kinase C

Hu, Ting‐Jun; Zheng, Rong

doi:10.1016/j.intimp.2003.11.001

Cited by 15 publications

(4 citation statements)

References 32 publications

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“…The secretion of NO was studied by a spectrophotometric assay based on the Griess reaction [30]. Briefly, 100 μl of the culture supernatant was mixed with an equal volume of Griess reagent (freshly mixed 0.1% naphthylethylenediamine dihydrochloride solution and 1% sulphanilamide in 5% H 3 PO 4 solution in a volume ratio of 1:1) at room temperature.…”

Section: Methodsmentioning

confidence: 99%

Effect of total flavonoids of Spatholobus suberectus Dunn on PCV2 induced oxidative stress in RAW264.7 cells

Chen

Yang

et al. 2017

BMC Complement Altern Med

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BackgroundThis study was carried out to investigate the effect of total flavonoids of Spatholobus suberectus Dunn (TFSD) on PCV2 induced oxidative stress in RAW264.7 cells.MethodsOxidative stress model was established in RAW264.7 cells by infecting with PCV2. Virus infected cells were then treated with various concentrations (25 mg/ml, 50 mg/ml and 100 mg/ml) of TFSD. The levels of oxidative stress related molecules (NO, ROS, GSH and GSSG) and activities of associated enzymes (SOD, MPO and XOD were analyzed using ultraviolet spectrophotometry, fluorescence method and commercialized detection kits.ResultsPCV2 infection induced significant increase of NO secretion, ROS generation, GSSG content, activities of both XOD and MPO, and dramatically decrease of GSH content and SOD activity in RAW264.7 cells (P < 0.05). After treating with TFSD, PCV2 induced alteration of oxidative stress related molecule levels and enzyme activities were recovered to a level similar to control.ConclusionOur findings indicated that TFSD was able to regulate oxidative stress induced by PCV2 infection in RAW264.7 cells, which supports the ethnomedicinal use of this herb as an alternative or complementary therapeutic drug for reactive oxygen-associated pathologies.

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Section: Methodsmentioning

confidence: 99%

Effect of total flavonoids of Spatholobus suberectus Dunn on PCV2 induced oxidative stress in RAW264.7 cells

Chen

Yang

et al. 2017

BMC Complement Altern Med

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“…Both GLP-2 36 and FPS-1 37 can promote the proliferation of splenic lymphocytes in vitro and can enhance the activity of T cells and B cells in mice after intraperitoneal injection. Lycium barbarum polysaccharide (LBP), loach mucilage polysaccharide, 38 Cordyceps polysaccharide, 39 Sophora japonica polysaccharide 40 and Coriolus versicolor polysaccharide 41 can stimulate T cell proliferation and secrete IL-2 and other cytokines in vivo and in vitro , but they have no effect on B cells. The Cordyceps -induced proliferation of T cells has been shown to be mediated by mitogen-activated protein kinases (MAPKs).…”

Section: Protective Effect Of Dietary Polysaccharides On the Intestin...mentioning

confidence: 99%

A critical review on intestinal mucosal barrier protection effects of dietary polysaccharides

Yan,

Luo,

et al. 2024

Food Funct.

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“…(2) PTK can induce the nuclear translocation of activator protein 1 (AP1) via the MAPK signaling pathway (ERK/JNK/p38). As a transcription factor associated with immune regulation, AP1 in the nucleus can induce gene expression, such as IL-4, IL-6, and IL-8 ( 67 , 68 ).…”

Section: Role Of Tcm Polysaccharides Played In Regulating Tumor Adapt...mentioning

confidence: 99%

Antitumor immunostimulatory activity of the traditional Chinese medicine polysaccharide on hepatocellular carcinoma

Liu,

Wu,

Hao

2024

Front. Immunol.

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Hepatocellular carcinoma (HCC) is a prevalent malignancy, often associated with compromised immune function in affected patients. This can be attributed to the secretion of specific factors by liver cancer cells, which hinder the immune response and lead to a state of immune suppression. Polysaccharides derived from traditional Chinese medicine (TCM) are valuable constituents known for their immunomodulatory properties. This review aims to look into the immunomodulatory effects of TCM polysaccharides on HCC. The immunomodulatory effects of TCM polysaccharides are primarily manifested through the activation of effector T lymphocytes, dendritic cells, NK cells, and macrophages against hepatocellular carcinoma (HCC) both in vivo and in vitro settings. Furthermore, TCM polysaccharides have demonstrated remarkable adjuvant antitumor immunomodulatory effects on HCC in clinical settings. Therefore, the utilization of TCM polysaccharides holds promising potential for the development of novel therapeutic agents or adjuvants with advantageous immunomodulatory properties for HCC.

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Promotion of Sophora subprosrate polysaccharide on nitric oxide and interleukin-2 production in murine T lymphocytes: implicated Ca2+ and protein kinase C

Cited by 15 publications

References 32 publications

Effect of total flavonoids of Spatholobus suberectus Dunn on PCV2 induced oxidative stress in RAW264.7 cells

Effect of total flavonoids of Spatholobus suberectus Dunn on PCV2 induced oxidative stress in RAW264.7 cells

A critical review on intestinal mucosal barrier protection effects of dietary polysaccharides

Antitumor immunostimulatory activity of the traditional Chinese medicine polysaccharide on hepatocellular carcinoma

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