Promotion of remyelination by adipose mesenchymal stem cell in cuprizone model of demyelination

Hedayatpour, Azim; Ragerdi, Iraj; Pasbakhsh, Parichehr; Kafami, Laya

doi:10.1016/j.jneuroim.2014.08.502

Cited by 25 publications

(35 citation statements)

References 32 publications

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“…In the cuprizone model, new oligodendrocytes and new myelin occur first at week 5 (Matsushima and Morell, 2001;Torkildsen et al, 2008;Kipp et al, 2009;Skripuletz et al, 2011a). In another study Hedayatpour et al, 2013 showed beneficial effects of intravenously applied MSC on remyelination. However, the authors performed a six week cuprizone diet to induce demyelination.…”

Section: Figmentioning

confidence: 94%

“…In the cuprizone model, regeneration of oligodendrocytes and new myelin occur despite of continuous cuprizone exposure at week 6 (Gudi et al, 2009;Matsushima and Morell, 2001;Kipp et al, 2009;Skripuletz et al, 2011a). In the work of Hedayatpour et al (2013) the corpus callosum was dissected and a single cell suspension was prepared for FACS analyses of glial reactions including OPC. Although, this method is suitable to isolate microglia, analysis of OPC and astrocytes seems to be rather artificial.…”

Section: Figmentioning

confidence: 99%

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Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system

Tejedor

Berner

Jacobsen

et al. 2015

Brain, Behavior, and Immunity

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Section: Figmentioning

confidence: 94%

Section: Figmentioning

confidence: 99%

Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system

Tejedor

Berner

Jacobsen

et al. 2015

Brain, Behavior, and Immunity

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“…Their relatively non-complicated procurement and collection (often performed as liposuction during aesthetic surgery) and grand plasticity support this idea even more. For example, Hedayatpour et al (2013) stated that ATSCs promoted renewal of damaged myelin sheaths around nerve fibres in mice with artificially induced sclerosis multiplex. Also Wang et al (2014) described improved regeneration ability of the damaged articular cartilages after therapy with ATSCs in rabbits.…”

Section: Introductionmentioning

confidence: 99%

Ultra-structural morphology of long-term cultivated white adipose tissue-derived stem cells

et al. 2015

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White adipose tissue was long perceived as a passive lipid storage depot but it is now considered as an active and important endocrine organ. It also harbours not only adipocytes and vascular cells but also a wide array of immunologically active cells, including macrophages and lymphocytes, which may induce obesity-related inflammation. Recently, adipose tissue has been reported as a source of adult mesenchymal stem cells with wide use in regenerative medicine and tissue engineering. Their relatively non-complicated procurement and collection (often performed as liposuction during aesthetic surgery) and grand plasticity support this idea even more. We focused our research on exploring the issues of isolation and long-term cultivation of mesenchymal stem cells obtained from adipose tissue. Ultra-structural morphology of the cells cultivated in vitro has been studied and analysed in several cultivation time periods and following serial passages--up to 30 passages. In the first passages they had ultra-structural characteristics of cells with high proteosynthetic activity. Within the cytoplasm, big number of small lipid droplets and between them, sparsely placed, small and inconspicuous, electron-dense, lamellar bodies, which resembled myelin figures were observed. The cells from the later passages contained high number of lamellar electron-dense structures, which filled out almost the entire cytoplasm. In between, mitochondria were often found. These bodies were sometimes small and resembled myelin figures, but several of them reached huge dimensions (more than 1 µm) and their lamellar structure was not distinguishable. We did not have an answer to the question about their function, but they probably represented the evidence of active metabolism of lipids present in the cytoplasm of these cells or represented residual bodies, which arise after the breakdown of cellular organelles, notably mitochondria during long-term cultivation.

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“…Even more significant for the neurological context, MSCs are able to express a variety of neuro-regulatory molecule genes, such as the axon guidance molecules, neural cell adhesion molecules, neuriteinducing factors, neurotransmitter receptors, and neurotrophic factors [191]. Thus, MSCs of different origin have become an attractive pursuit in the studies on animal models of stem cell-based therapies in stroke (i.e., BM-MSCs) [192,193], traumatic brain injury (i.e., BM-MSCs) [194], and in several models of neurological disorders, such as in Parkinson's disease (PD) (i.e., UC-MSCs) [195], amyotrophic lateral sclerosis (ALS) (i.e., BM-MSCs) [196], multiple sclerosis (MS) (i.e.,ASCs) [197], Alzheimer's disease (AD) (i.e.,ASCs) [198], and Huntington disease (HD) (i.e., UC-MSCs) [199].…”

Section: Mscs In Neural Differentiation and Repairmentioning

confidence: 99%

Genetic Engineering of Mesenchymal Stem Cells for Regenerative Medicine

Nowakowski

Walczak

Janowski

et al. 2015

Stem Cells and Development

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Mesenchymal stem cells (MSCs), which can be obtained from various organs and easily propagated in vitro, are one of the most extensively used types of stem cells and have been shown to be efficacious in a broad set of diseases. The unique and highly desirable properties of MSCs include high migratory capacities toward injured areas, immunomodulatory features, and the natural ability to differentiate into connective tissue phenotypes. These phenotypes include bone and cartilage, and these properties predispose MSCs to be therapeutically useful. In addition, MSCs elicit their therapeutic effects by paracrine actions, in which the metabolism of target tissues is modulated. Genetic engineering methods can greatly amplify these properties and broaden the therapeutic capabilities of MSCs, including transdifferentiation toward diverse cell lineages. However, cell engineering can also affect safety and increase the cost of therapy based on MSCs; thus, the advantages and disadvantages of these procedures should be discussed. In this review, the latest applications of genetic engineering methods for MSCs with regenerative medicine purposes are presented.

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Promotion of remyelination by adipose mesenchymal stem cell in cuprizone model of demyelination

Cited by 25 publications

References 32 publications

Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system

Mesenchymal stem cells do not exert direct beneficial effects on CNS remyelination in the absence of the peripheral immune system

Ultra-structural morphology of long-term cultivated white adipose tissue-derived stem cells

Genetic Engineering of Mesenchymal Stem Cells for Regenerative Medicine

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