Promotion of Health by Soy Isoflavones: Efficacy, Benefit and Safety Concerns

Goldwyn, Shira; Lazinsky, Alison; Wei, Huachen

doi:10.1515/dmdi.2000.17.1-4.261

Cited by 58 publications

(34 citation statements)

References 86 publications

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“…As its chemical structure relates to estradiol, an endogenous estrogen, genistein can bind to estrogen receptors [2] and exert weak estrogenic and/or antiestrogenic effects on numerous cells and tissues both in vitro and in vivo [3]. Consuming soy-based foods has been suggested to have potential health benefits; for example, reduced risks of cardiovascular diseases, lower blood pressure, reduced incidences of osteoporosis, and reduced risks for breast and endometrial cancers [4,5]. However, its underlying mechanisms are unclear and some adverse effects of genistein on the female reproductive tract have been reported [6,7].…”

Section: Introductionmentioning

confidence: 99%

Activation of Chloride Secretion by Isoflavone Genistein in Endometrial Epithelial Cells

Deachapunya¹,

Poonyachoti²

2013

Cell Physiol Biochem

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Background /Aim: Genistein, the most active isoflavone found primarily in soybeans, alters ion transport functions in intestinal and airway epithelia. The present study aims to investigate the acute effects and mechanisms of action of genistein in immortalized porcine endometrial epithelial cells. Methods: Ussing chamber technique was used for transepithelial electrical measurements. Results: Genistein increased short-circuit currents (I_sc) which were inhibited by glibenclamide, NPPB, CFTRinh-172, DIDS or bumetanide, but not amiloride. In experiments with amphotericin B-permeabilized monolayers, genistein activated the apical Cl^- current and barium-sensitive basolateral K⁺ current while inhibiting the apical K⁺ current. Genistein failed to increase the I_sc in the presence of forskolin or IBMX, but did increase the I_sc in UTP. Pretreatment with genistein also abolished the increase in the I_sc when induced by forskolin, IBMX or UTP. However, Ca²⁺-chelating BAPTA-AM did not affect the genistein-induced increase in the I_sc. The genistein-stimulated I_sc was reduced by tyrosine kinase inhibitors, tyrphostin A23 or AG490. However, vanadate, a tyrosine phosphatase inhibitor, failed to inhibit the genistein response. Estrogen receptor antagonist ICI182,780 did not alter the genistein's action. Conclusion: The soy isoflavone, genistein, stimulates Cl^- secretion in endometrial epithelial cells possibly via a direct activation of CFTR which appears to be modulated through a tyrosine kinase-dependent pathway. The present findings may be of benefit for the therapeutic application of genistein in the treatment of electrolyte transport disorders in the epithelia.

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Section: Introductionmentioning

confidence: 99%

Activation of Chloride Secretion by Isoflavone Genistein in Endometrial Epithelial Cells

Deachapunya¹,

Poonyachoti²

2013

Cell Physiol Biochem

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“…The pleiotropic effects of genistein remain controversial. Whereas some laboratories report therapeutic effects of genistein such as chemoprevention, improved bone health, and amelioration of menopausal symptoms (Goldwyn et al, 2000;Evans et al, 2011), other groups emphasize the phytoestrogen's adverse health effects, namely endocrine disruption (Casanova et al, 1999;Jefferson et al, 2005;Wisniewski et al, 2005). In both humans and rats, genistein causes precocious puberty and altered menstrual cycles (Casanova et al, 1999;Strom et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of Genistein Glucuronidation by Bisphenol A in Human and Rat Liver Microsomes

Coughlin¹,

Thomas²,

Buckley³

2011

Drug Metab Dispos

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ABSTRACT:Genistein is a natural phytoestrogen of the soybean, and bisphenol A (BPA) is a synthetic chemical used in the production of polycarbonate plastics. Both genistein and BPA disrupt the endocrine system in vivo and in vitro. Growing concerns of altered xenobiotic metabolism due to concomitant exposures from soy milk in BPAladen baby bottles has warranted the investigation of the glucuronidation rate of genistein in the absence and presence (

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“…Genistein (4′,5,7-trihydroxyflavone), a naturally occurring isoflavone found in soybeans, has gained increasing attention because of its association with beneficial effects for persons with cardiovascular disease, high cholesterol levels, osteoporosis, breast cancer and prostate cancer (Goldwyn et al, 2000). In addition, this isoflavone provides protection against non-ionizing ultraviolet-B radiation when applied to the skin of hairless mice 1 h before exposure (Wei et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Genistein treatment protects mice from ionizing radiation injury

Landauer¹,

Srinivasan²,

Seed³

2003

J of Applied Toxicology

128

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The radioprotective and behavioral effects of an acute administration of the isoflavone genistein (4',5,7-trihydroxyflavone) were investigated in adult CD2F1 male mice. Mice were administered a single subcutaneous (s.c.) dose of genistein either 24 h or 1 h before a lethal dose of gamma radiation (9.5-Gy of cobalt-60 at 0.6 Gy min(-1)). Mice received saline, PEG-400 vehicle or genistein at 3.125, 6.25, 12.5, 25, 50, 100, 200, or 400 mg kg(-1) body weight. For mice treated 24 h before irradiation there was a significant increase in 30-day survival for animals receiving genistein doses of 25 to 400 mg kg(-1) (p<0.001). In contrast, the 30-day survival rates of mice treated with genistein 1 h before irradiation were not significantly different from those of the vehicle control group. Additionally, the acute toxicity of genistein was evaluated in non-irradiated male mice administered a single s.c. injection of saline, vehicle, or genistein at 100, 200 or 400 mg kg(-1). At these genistein doses there were no adverse effects, compared with controls, on locomotor activity, grip strength, motor coordination, body weight, testes weight, or histopathology. These results demonstrate that a single s.c. administration of the flavonoid genistein at non-toxic doses provides protection against acute radiation injury.

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Promotion of Health by Soy Isoflavones: Efficacy, Benefit and Safety Concerns

Cited by 58 publications

References 86 publications

Activation of Chloride Secretion by Isoflavone Genistein in Endometrial Epithelial Cells

Activation of Chloride Secretion by Isoflavone Genistein in Endometrial Epithelial Cells

Inhibition of Genistein Glucuronidation by Bisphenol A in Human and Rat Liver Microsomes

Genistein treatment protects mice from ionizing radiation injury

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