Promotion of Fern Rhizoid Elongation by Metal Ions and the Function of the Spore Coat as an Ion Reservoir

Miller, John H.; Vogelmann, Thomas C.; Bassel, Alix R.

doi:10.1104/pp.71.4.828

Cited by 17 publications

(11 citation statements)

References 27 publications

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“…Ca is taken up over this time, however, since the gametophytes are assimilating carbon and the Ca content is expressed relative to the dry weight. Ca is needed later for rhizoid growth (18) and protonema development (4). The R-stimulated increase in total intracellular Ca can be reversed by a subsequent irradiation with FR indicating that the increase in intracellular Ca is mediated through phytochrome (Table III).…”

Section: Wayne and Heplermentioning

confidence: 99%

Red Light Stimulates an Increase in Intracellular Calcium in the Spores of Onoclea sensibilis

Wayne¹,

Hepler²

1985

Plant Physiol.

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Red light (R) stimulates an increase in the total concentration of intracellular calcium in the spores of Oaocka seaibilis L. as determined by atomic absorption spectoscopy. Subsequent exposure to far-red light inhibits the R-induced inrease in intracellular calcium. The majority of the incea occurs 5 minutes after the onset of irradiation. The calcium Intagonist, La3+, inhibits both germination and the R-induced increase in intracellular calcium. The R-induced increase in calcium is sufficient to account for an increase in the concentration of intracellular calcium ions from 0.1 micromolar to 1 to 10 mcromolar. ILage detectable changes in other elements tested are not required for germination.

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Section: Wayne and Heplermentioning

confidence: 99%

Red Light Stimulates an Increase in Intracellular Calcium in the Spores of Onoclea sensibilis

Wayne¹,

Hepler²

1985

Plant Physiol.

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“…The germination can take place in spores hydrated in just water (15), but it has been indicated that ions, particularly Ca2", present in the spore coat play essential roles for germination (16,17,24,25). In fact, spores become strictly dependent on the supply of external Ca2+ when treated with the Ca2+ chelator, EGTA (17,24 2 Abbreviations: R, red light; FR, far-red light; GO, G1, and S, the phases of the cell cycle.…”

mentioning

confidence: 99%

The Occurrence of a Ca²⁺-Dependent Period in the Red Light-Induced Late G1 Phase of Germinating Adiantum Spores

Iino¹,

Endo²,

Wada³

1989

Plant Physiol.

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The first cell cycle of Adiantum spores, which is induced by red light (R), was studied with regard to its temporal dependence on Pfr (the active form of phytochrome) and Ca2 . Responses were quantified with increases of the spore content of DNA, thus limiting the investigation to the reactions taking place before the S phase of the cell cycle (i.e. during GO/G1/S transitions). Spores hydrated for more than 2 days in the standard medium (includes 1 millimolar free Ca2+) showed, after stimulation with a saturating R pulse, an increase of DNA beginning at about 25 hours and saturating at about 55 hours. Reversal by far-red light of the inductive effect of R was used to examine the temporal requirement for Pfr. Spores became dependent on the supply of extemal Ca2+ when incubated in a low Ca medium (32 nanomolar free Ca2+ with 10 millimolar EGTA); this culture condition was used, after observing that the DNA increase occurs similarly if Ca2+ is supplied after the R pulse, to examine the temporal requirement for external Ca2 . It was concluded that the GI phase of the spore is separated into three subphases: (a) the Pfr-dependent period which immediately follows the R pulse and varies among individual spores from a few to about 25 hours, (b) the Ca2+-dependent period (about 10 hours) which occurs immediately before the S phase, and (c) a gap (15-20 hours) between the two periods. In the Ca2+-dependent period, spores require the presence of extracellular Ca2+. This period occurs only during a determined time after the R pulse, and the competence of spores to enter the S phase is lost sharply if external Ca2+ is not available continuously during this period.

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“…Figure 7 also shows the effect ofpCa on primary rhizoid elongation. Ca ++ is also required for spore germination in Onoclea sensibilis (Miller and Wagner, 1987) and Adiantum capillusveneris (lino, Endo, and Wada, 1989). Ca ++-uptake via ion channels is required for cytokinin-induced bud formation in the moss, Funaria hygrometrica (Conrad and Hepler, 1988).…”

Section: Discussionmentioning

confidence: 99%

“…The standard medium used for the culture of gemmaẽ as Knop's medium (Miller and Greany, 1974), pH adjusted to 5.3-5.4, and prepared with twice-distilled water and reagent grade salts. EGTA was from Sigma Chemical Co, St. Louis, Missouri.…”

Section: Methodsmentioning

confidence: 99%

Calcium Delays the Onset of Cell Division and Rhizoid Elongation in Gemmae of the Calcifuge Fern, Vittaria Graminifolia

Sabba¹,

Miller²

1993

American J of Botany

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Vittaria graminifolia gametophytes reproduce asexually by linear six‐celled propagules known as gemmae. It has been shown previously that asymmetric cell division and rhizoid elongation in gemmae are inhibited by millimolar concentrations of Ca+ +. The present paper shows that millimolar Ca+ + delays the onset of cell division at a point prior to mitosis, without affecting the maximal rate of cell division. Ca+ + is most effective in delaying cell division when it is present during the first 24 hours of culture, 2 or 3 days before the initiation of cell division. Millimolar Ca+ + inhibits rhizoid elongation by delaying its onset, while also reducing its maximal rate. Ca+ + is also most effective in delaying rhizoid elongation during the first 24 hours of culture. Culture of gemmae on Ethylglycol‐bis‐(aminoethyl ether)‐N,N,N‘,N‘‐tetraacetic acid‐buffered media shows that the maximum frequency of cell division occurs at pCa 7, while for the initiation of rhizoid elongation, it occurs at pCa 5.

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Promotion of Fern Rhizoid Elongation by Metal Ions and the Function of the Spore Coat as an Ion Reservoir

Abstract: ABSTRACT

Cited by 17 publications

References 27 publications

Red Light Stimulates an Increase in Intracellular Calcium in the Spores of Onoclea sensibilis

Red Light Stimulates an Increase in Intracellular Calcium in the Spores of Onoclea sensibilis

The Occurrence of a Ca²⁺-Dependent Period in the Red Light-Induced Late G1 Phase of Germinating Adiantum Spores

Calcium Delays the Onset of Cell Division and Rhizoid Elongation in Gemmae of the Calcifuge Fern, Vittaria Graminifolia

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Promotion of Fern Rhizoid Elongation by Metal Ions and the Function of the Spore Coat as an Ion Reservoir

Abstract: ABSTRACT

Cited by 17 publications

References 27 publications

Red Light Stimulates an Increase in Intracellular Calcium in the Spores of Onoclea sensibilis

Red Light Stimulates an Increase in Intracellular Calcium in the Spores of Onoclea sensibilis

The Occurrence of a Ca2+-Dependent Period in the Red Light-Induced Late G1 Phase of Germinating Adiantum Spores

Calcium Delays the Onset of Cell Division and Rhizoid Elongation in Gemmae of the Calcifuge Fern, Vittaria Graminifolia

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The Occurrence of a Ca²⁺-Dependent Period in the Red Light-Induced Late G1 Phase of Germinating Adiantum Spores