Promotion of elongation and acid invertase activity in Phaseolus vulgaris L. internode segments by phenylacetic acid

Abstract

Phenylacetic acid (PAA) significantly stimulated the elongation of isolated Phaseolus vulgaris internodal segments and prevented the decline in acid invertase specific activity observed in segments incubated in the absence of growth substances. Unlike MA, which stimulated both elongation and invertase activity over a very wide range of concentrations (< low4 -1 mol.mA3; optimum 10-2mol.m-3), the response to PAA was restricted to a much narrower range of concentrations (3 x lo-' -1 mol.m-'; optimum ca. l-2 x 10-'mol.m-3). At the optimum concentration of PAA, the stimulation of both responses was about 63-75% of that induced by the optimum concentration of IAA. The differences in the concentration range and magnitude of the responses to IAA and PAA were not due to differences in uptake of the two compounds. The stimulation of elongation by both compounds was prevented by 3.6 x lo-*mol.m-' cycloheximide (CH), and acid invertase activites were greatly reduced compared with samples treated with growth substances alone. A saturating concentration of the specific auxin efflux carrier inhibitor N-1-naphthylphthalamic acid (NPA) slightly promoted the growth of control segments, probably by reducing the loss of residual endogenous auxin to the incubation medium. The elongation induced by PAA at its optimum concentration was considerably greater than the elongation induced by NPA, indicating that PAA did not cause growth by preventing the loss of endogenous auxin from the segments. Elongation responses to combinations of IAA and PAA suggested that the compounds were acting additively and that they were affecting growth by the same mechanism.

The effect of phenyl acetic acid on shoot bud induction, elongation and rooting of chickpea

“…Between the two cultivars, ICCV-10 produced more shoots per culture compared to Annigeri (Table 1) only in some hormone combinations, suggesting genotypic dependency on shoot regeneration as earlier reported in other legumes (Ma and Wu 2008). Small and Morris (1990) and Husain et al (1999) reported that PAA had a positive effect in producing normal, elongated shoots in Phaseolus vulgaris and Capsicum annuum. A large number of shoot buds differentiated within 3 -4 weeks in the primary shoot-bud induction medium (PAA + BAP).…”

Soluble and insoluble invertase activity in elongating Tulipa gesneriana flower stalks

“…Rapid tissue growth has often been correlated with elevated soluble (Morris and Arthur 1984a and references therein, Morris and Arthur 1985, 1986, Small and Morris 1990, Arai et al 1991) and insoluble (Chin and Weston 1973, Weil and Rausch 1990, Getz 1991) invertase activities in homogenates. It is thought that invertases may serve to maintain the sucrose concentration gradient between sieve tubes and sink cells and thus stimulating phloem unloading of sucrose (Eschrich 1980, Morris andArthur 1984a).…”

“…Acid invertase activity is often increased in rapidly growing tissues (Arai et al 1991, Morris andArthur 1984a andreferences therein). Its induction can be under hormonal control, but direct evidence of a causal relation-ship between hormone-induced invertase activity and growth is still lacking (Kaufman et al 1968, Seitz and Lang 1968, Nowak and Rudnicki 1976, Morris and Arthur 1984b, 1985, 1986, Small and Morris 1990. Attempts have been made to locate invertase activity in the cell.…”

Phenyl acetic acid‐induced in vitro shoot multiplication of Vanilla planifolia

“…Davidonis and Knorr (1991) tried to induce callus formation and subsequent shoot regeneration. Phenyl acetic acid (PAA) showed auxin-like activity with wheat and Avena coleoptiles (Small and Morris 1990), induced stem elongation, stimulation of adventitious shoots and somatic embryogenesis (Slimmon et al 1991;Jethwani and Kothari 1996). This study concerns the influence of phenyl acetic acid on in vitro shoot multiplication of V. planifolia from nodal explants containing axillary buds.…”