Promotion of cutaneous wound healing by local application of mesenchymal stem cells derived from human umbilical cord blood

Abstract

This study aimed to determine whether mesenchymal stem cells (MSCs) derived from umbilical cord blood (UCB) would promote cutaneous wound healing. MSCs from human UCB were isolated and identified. The characteristics of the isolated MSCs' growth and proliferation were assayed in vitro. The MSCs labeled with 5-bromodeoxyuridine (BrdU) were applied on fresh cutaneous mice wounds. The healing rates were surveyed. The distribution and the differentiation into keratinocytes of the labeled MSCs in the wound tissue were checked by immunohistochemistry staining. The isolated MSCs could grow and proliferate well in vitro. The isolated MSCs from UCB could be labeled by 5-bromodeoxyuridine successfully. The MSCs derived from UCB could enhance the healing of mice skin defect wounds, and it was found that the implanted MSCs could differentiate into keratinocyte in the wound tissue. It was demonstrated that MSCs from UCB can be isolated and proliferated successfully. The local administration of MSCs derived from UCB improves skin defect wound healing in mice.

A Chemically Defined Carrier for the Delivery of Human Mesenchymal Stem/Stromal Cells to Skin Wounds

“…6,7 Recently MSCs have been shown to support cutaneous wound regeneration by accelerating wound closure, reducing scarring, and restoring the final tensilestrength of the tissue to levels higher than untreated wounds. [8][9][10][11][12][13][14] Also their well-documented immunomodulatory and anti-inflammatory properties 15 make these cells an attractive cell source for treating chronic wounds.…”

Topical Application of Human Umbilical Cord Blood in Chronic Wounds: A Clinical and Prospective Study

“…5 It has been observed that the local administration of Mesenchymal Stem Cells (MSCs) derived from Umbilical Cord Blood (UCB) improves skin wound healing in mice and it was found that the implanted MSCs could differentiate into keratinocytes in the wound. 6 We thought of using Human…”

Human umbilical cord-derived mesenchymal stem cells differentiate into epidermal-like cells using a novel co-culture technique

“…hUC blood-derived MSCs enhanced wound healing in mice by differentiating into keratinocytes (Luo et al 2010). Although it is known that bone marrow-derived MSCs (BMSCs) will home to the injury sites and contribute to keratinocytes for skin regeneration in vivo after skin injury (Borue et al 2004;Satoh et al 2004;Badiavas et al 2003) and differentiate into epidermal lineage in 3D organotypic co-culture (Ma et al 2009), similar analyses have not been performed using hUCMSCs.…”