Promotion of bone repair of rabbit tibia defects induced by scaffolds of $$\hbox {P(VDF-TrFE)/BaTiO}_{3}$$ composites

Abstract

In this work, scaffolds made of a novel experimental 0-3 type composite were implanted onto non-critical defects in rabbit tibiae. This work discusses the bone repair promoted by polyvinylidene fluoride-trifluoroethylene P(VDF-TrFE)/barium titanate (BaTiO 3) composite that scaffolds with 10 vol% BaTiO 3. Prior to implant surgery, the P(VDF-TrFE)/BaTiO 3 scaffolds, moulded into a membrane disk, were subjected to a cytotoxicity test (ASTM F895-84). A standardized transverse osteotomy was made with the following dimensions: 4.5 mm in width by 9 mm in length, at the proximal tibial metaphysis, in adult male rabbits, by using a cylindrical drill, cooled with a physiologic solution. These critical defects were filled with blood clot on the left tibiae (control group), whereas the right tibiae were covered with composite scaffolds, measuring 5 mm in thickness and 10 mm in diameter (experimental group); n = 12 for each group. After 21 days, the rabbits were sacrificed and the tibiae bone fragments were conducted to demineralization routines, from fixation and stain procedures to histological analysis. The scaffolds promote the growth of the bone, resulting in an increased repair with callus formation around the scaffold and high mitotic activity at newly formed bones.

Surface Modification of Ti–30Ta Alloy by Deposition of P(VDF-TrFE)/BaTiO3 Coating for Biomedical Applications

“…According to previous works related to the evaluation of biocompatibility of P(VDF-TrFE)BaTiO 3 membranes developed by the research group led by Professor Gimenes, the surface of P(VDF-TrFE)BaTiO 3 was shown to be osteogenic. Supported by published findings [28,34], we assume that Ti-30Ta coated with P(VDF-TrFE)/BaTiO 3 film can provide an osteogenic surface.…”

“…In addition, this ceramic material presents higher piezoelectricity than PVDF [30,31]. In this context, the combination of BaTiO 3 with a copolymer creates a composite P(VDF-TrFE)/BaTiO 3, which has electrical characteristics that promote bone regeneration [27,[32][33][34]. Considering these benefits and possible solutions, the purpose of the present study was to develop a low-cost methodology to coat Ti-30Ta alloys using P(VDF-TrFE)/BaTiO 3 films by employing a spray coating technique at room temperature.…”

Histomorphometric evaluation of 3D printed graphene oxide-enriched poly(ε-caprolactone) scaffolds for bone regeneration

“…The periosteum was repositioned followed by wound suturing, Fig. 3 [ 22 ]. Rabbits were given 0.2 ml of penicillin subcutaneously and ketoprofen (3 mg/kg body weight) intramuscularly for 5 days post-surgery (Eipico pharma, Egypt).…”

Investigation of the impact of planar microelectrodes on macrophage-mediated mesenchymal stem cell osteogenesis

“…To enhance its ferroelectric and piezoelectric attributes, researchers (Nunes-Pereira et al, 2015) introduced the trifluoroethylene monomer (TrFE) into PVDF, resulting in the copolymer Poly(vinylidene fluoridetrifluoroethylene) (P(VDF-TrFE)), which exhibit improved charge accumulation and storage capabilities, enabling the acquisition of a permanent surface potential. P(VDF-TrFE) is capable of carrying surface charge and has been demonstrated to promote osteogenesis in MSCs in vitro (Luo et al, 2022;Zhang et al, 2022), enhance bone repair in vivo (Gimenes et al, 2019;Zhang et al, 2022), and facilitate macrophage polarization (Dai et al, 2021). The application of an external power source through ITO planar microelectrodes can impart a continuous electrical stimulation to cells or tissues, thereby influencing their morphology and function.…”

“…P(VDF-TrFE) is capable of carrying surface charge and has been demonstrated to promote osteogenesis in MSCs in vitro ( Luo et al, 2022 ; Zhang et al, 2022 ), enhance bone repair in vivo ( Gimenes et al, 2019 ; Zhang et al, 2022 ), and facilitate macrophage polarization ( Dai et al, 2021 ). The application of an external power source through ITO planar microelectrodes can impart a continuous electrical stimulation to cells or tissues, thereby influencing their morphology and function.…”