Promoting Wound Healing Using Low Molecular Weight Fucoidan in a Full-Thickness Dermal Excision Rat Model

Park, Jun-Hyeong; Choi, Seong-Hun; Park, Soo‐Jin; Lee, Young Joon; Park, Jong Hyun; Song, Phil Hyun; Cho, Chang-Mo; Ku, Sae‐Kwang; Song, Chang‐Hyun

doi:10.3390/md15040112

Cited by 81 publications

(53 citation statements)

References 53 publications

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“…Future experiments will focus on the early phases of this inflammatory process, in particular the kinetics of neutrophil recruitment to the infected footpad and DRG neurons. It would be interesting to assess the therapeutic efficacy of inhibitors of neutrophil recruitment, such as fucoidan, on the immunopathology of PRV in mice (62).…”

Section: Discussionmentioning

confidence: 99%

Virulent Pseudorabies Virus Infection Induces a Specific and Lethal Systemic Inflammatory Response in Mice

Laval

Vernejoul

Cleemput

et al. 2018

J Virol

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Pseudorabies virus (PRV) is an alphaherpesvirus that infects the peripheral nervous system (PNS). The natural host of PRV is the swine, but it can infect most mammals, including cattle, rodents, and dogs. In these nonnatural hosts, PRV always causes a severe acute and lethal neuropathy called the "mad itch," which is uncommon in swine. Thus far, the pathophysiological and immunological processes leading to the development of the neuropathic itch and the death of the animal are unclear. Using a footpad inoculation model, we established that mice inoculated with PRV-Becker (virulent strain) develop a severe pruritus in the foot and become moribund at 82 h postinoculation (hpi). We found necrosis and inflammation with a massive neutrophil infiltration only in the footpad and dorsal root ganglia (DRGs) by hematoxylin and eosin staining. PRV load was detected in the foot, PNS, and central nervous system tissues by quantitative reverse transcription-PCR. Infected mice had elevated plasma levels of proinflammatory cytokines (interleukin-6 [IL-6] and granulocyte colony-stimulating factor [G-CSF]) and chemokines (Gro-1 and monocyte chemoattractant protein 1). Significant IL-6 and G-CSF levels were detected in several tissues at 82 hpi. High plasma levels of C-reactive protein confirmed the acute inflammatory response to PRV-Becker infection. Moreover, mice inoculated with PRV-Bartha (attenuated, live vaccine strain) did not develop pruritus at 82 hpi. PRV-Bartha also replicated in the PNS, and the infection spread further in the brain than PRV-Becker. PRV-Bartha infection did not induce the specific and lethal systemic inflammatory response seen with PRV-Becker. Overall, we demonstrated the importance of inflammation in the clinical outcome of PRV infection in mice and provide new insights into the process of PRV-induced neuroinflammation.IMPORTANCE Pseudorabies virus (PRV) is an alphaherpesvirus related to human pathogens such as herpes simplex virus 1 and varicella-zoster virus (VZV). The natural host of PRV is the swine, but it can infect most mammals. In susceptible animals other than pigs, PRV infection always causes a characteristic lethal pruritus known as the "mad itch." The role of the immune response in the clinical outcome of PRV infection is still poorly understood. Here, we show that a systemic host inflammatory response is responsible for the severe pruritus and acute death of mice infected with virulent PRV-Becker but not mice infected with attenuated strain PRV-Bartha. In addition, we identified IL-6 and G-CSF as two main cytokines that play crucial roles in the regulation of this process. Our findings give new insights into neuroinflammatory diseases and strengthen further the similarities between VZV and PRV infections at the level of innate immunity. Citation Laval K, Vernejoul JB, Van Cleemput J, Koyuncu OO, Enquist LW. 2018. Virulent pseudorabies virus infection induces a specific and lethal systemic inflammatory response in mice. J Virol 92:e01614-18. https://doi.org/10 .1128/JVI.01614-18. Editor Ric...

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Section: Discussionmentioning

confidence: 99%

Virulent Pseudorabies Virus Infection Induces a Specific and Lethal Systemic Inflammatory Response in Mice

Laval

Vernejoul

Cleemput

et al. 2018

J Virol

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“…Lee et al [133] investigated the wound healing properties of alginate in the treatment of full-thickness skin defects in rats and found significant decrease of wound sizes in alginate treated groups. Fucoidan alone or in combination with chitosan also showed the best reepithelization and fastest wound closure results in rabbit dermal burns [134]. On the other hand, Custódio et al [135] have shown that laminarin can be used for the formation of biocompatible, mechanically stable and injectable hydrogels that can be used for soft tissue repair.…”

Section: Brown Algae Sulfated Polysaccharides As Drug Delivery Systemmentioning

confidence: 99%

Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

et al. 2020

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Over the years, brown algae bioactive polysaccharides laminarin, alginate and fucoidan have been isolated and used in functional foods, cosmeceutical and pharmaceutical industries. The extraction process of these polysaccharides includes several complex and time-consuming steps and the correct adjustment of extraction parameters (e.g., time, temperature, power, pressure, solvent and sample to solvent ratio) greatly influences the yield, physical, chemical and biochemical properties as well as their biological activities. This review includes the most recent conventional procedures for brown algae polysaccharides extraction along with advanced extraction techniques (microwave-assisted extraction, ultrasound assisted extraction, pressurized liquid extraction and enzymes assisted extraction) which can effectively improve extraction process. The influence of these extraction techniques and their individual parameters on yield, chemical structure and biological activities from the most current literature is discussed, along with their potential for commercial applications as bioactive compounds and drug delivery systems.(APS) is determined by algae species however it is also influenced by other factors causing inter-species variation, e.g., growth location and harvesting season [8]. Vast structural variation between the APS therefore presents a challenge in terms of pre-treatments application, extraction techniques and optimization, characterization of isolated fractions and determination of their biological properties.Chemical structure and yield of APS isolated from marine macroalgae by conventional extraction (CE) techniques can be affected by various experimental conditions (pH, time, temperature, pressure, particle size, solvent, sample to solvent ratio, agitation speed etc.). In addition, different advanced techniques such as microwave assisted extraction (MAE), ultrasound assisted extraction (UAE), pressurized liquid extraction (PLE), enzyme-assisted extractions (EAE) are assessed and applied for APS extraction [9][10][11].In general, the chemical structure of polysaccharides determines its physical, chemical and biochemical properties as well as its biological activities [12]. Several studies have reported that their biological activity is strongly associated with their chemical structure [9]. Due to very complex mechanisms that are affected by many factors, the correlation between polysaccharide structure and biological activity is still not sufficiently clarified.In order to improve isolation of APS, pre-treatments are usually applied to the algal biomass prior to the extraction process with the two aims: (i) to prevent co-extraction of interfering bioactive compounds with similar solubility; and (ii) to disrupt cell walls and improve mass transfer of APS into extraction solvent. The first type of pre-treatments is therefore used to remove compounds which are highly bound to the APS such as proteins, phenols and lipids, as well as mannitol and chlorophyll [13]. For that purpose, the application of various pr...

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“…Therefore, this study demonstrated apparent anti-bacterial and cutaneous wound healing effects of fucoidan. Fucoidan is known for its anti-bacterial, anti-viral, immunostimulatory, and healing stimulating effects [2][3][4][5][6][7][8][9][10][11]. In a previous study, fucoidan inhibited the growth of Staphylococcus aureus and Escherichia coli [5].…”

Section: Discussionmentioning

confidence: 99%

“…Fucoidan was also used as a prevention and treatment measure to control diseases caused by Helicobacter pylori [4]. Similutaneously, previous liteature demonstrated the cutaneous wound healing effect of fucoidan [10,11].…”

Section: Discussionmentioning

confidence: 99%

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Can Fucoidan Decrease the Mortalities Caused by Columnaris Disease in Nile Tilapia?

Mahgoub¹

2017

WJAR

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Columnaris disease is a serious disease in warm water fish. It is caused by Flavobacterium columnare, a Gram-negative bacterium. In this work, fucoidan was tested for its efficacy in decreasing the mortalities caused by Columnaris disease in Nile tilapia. Consequently, naturally-infected Nile tilapia with Flavobacterium columnare, showing eroded fins, rigid body figure, and ulcerated body surface, was treated with fucoidan (8 gm/kg ration) for 17 days. Flavobacterium columnare infection was confirmed by isolation on selective medium (cytophaga agar), from the skeletal muscle, tails fins, and gills, giving the typical rhizoid shape. It was also confirmed by Flavobacterium columnare specific PCR using selective primers for Flavobacterium columnare 16S ribosomal DNA. Fucoidan caused decreased the mortalities to nil and cured the eroded fins, the ulcerated body surface, and the rigid body figure. Fucoidan also decreased the tissue damage score to reach the normal histological score.

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Promoting Wound Healing Using Low Molecular Weight Fucoidan in a Full-Thickness Dermal Excision Rat Model

Cited by 81 publications

References 53 publications

Virulent Pseudorabies Virus Infection Induces a Specific and Lethal Systemic Inflammatory Response in Mice

Virulent Pseudorabies Virus Infection Induces a Specific and Lethal Systemic Inflammatory Response in Mice

Advanced Technologies for the Extraction of Marine Brown Algal Polysaccharides

Can Fucoidan Decrease the Mortalities Caused by Columnaris Disease in Nile Tilapia?

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