Promoting repair of highly purified stromal vascular fraction gel combined with advanced platelet-rich fibrin extract for irradiated skin and soft tissue injury

Li, Zhou; Gan, Huimin; Liang, Anru; Wang, Xiyue; Hu, Xiaohao; Liang, Ping; Xu, Guowen; Huang, Qianwen; Li, Junjun; Li, Hong-Mian

doi:10.21037/atm-22-3956

Cited by 3 publications

(3 citation statements)

References 43 publications

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“…A decrease in the density of microvessels in the skin, leading to tissue ischemia and, thereby, significantly reducing the rate of wound healing after 25 Gy X-irradiation was found in another study. In addition, the authors noted an increase in the expression of pro-inflammatory cytokines (TNF-α, IL-1, and IL-6), the release of cell adhesion molecules, which accelerated the migration, adhesion, and exudation of leukocytes and induced the migration of macrophages, neutrophils, and monocytes, which leads to a local inflammatory response to radiation injury ( Li et al , 2022 ). The polymorphonuclear infiltration of the skin found at various doses of single local electron irradiation can be explained by a similar mechanism the development of inflammation, the key role which is played by the imbalance of pro-inflammatory and anti-inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical and histochemical analysis of the rat skin after local electron irradiation

Demyashkin,

Shapovalova,

Marukyan

et al. 2023

Open Vet J

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Background: Skin cancer is the most frequently diagnosed type of cancer among all malignant neoplasms. The decrease in mitotic activity and the death of intact keratinocytes arise due to the constantly renewing epithelium is highly sensitive to ionising radiation. Aim: The aim of the study is immunohistochemical evaluation of the proliferative-apoptotic balance of keratinocytes, the fibrous component of the skin and the expression of pro-inflammatory and anti-inflammatory cytokines after single or fractional local electron irradiation. Methods: Wistar rats (n=80) were taken from the ITM&B Vivarium (Sechenov University) and divided into groups: I – control, which were injected with saline; and experimental groups, local electron irradiation at doses: II – 8 Gy (single), III – 40 Gy (single), IV – summary dose 78 Gy (fractional; 13 Gy per day for 6 days). We performed histological analysis, histochemical analysis using Masson, safranine and picrosirius red staining, immunohistochemical (Ki-67, caspase-3, p53, types I and III collagens, IL-1, IL-6, IL-4 and IL-10) and morphometric analysis of skin fragments of the outer surface of the thigh, irradiated in accordance with the design of the experiment. The early and delayed effects of local electron irradiation at different doses were studied. Results: After local electron irradiation, dose-dependent morphological changes in the skin of the experimental groups were observed: violation of the histoarchitectonics of the skin confirmed by morphological and morphometric analysis, proliferation of connective tissue according to the results of histochemical and immunohistochemical studies with signs of the radiation-induced skin fibrosis development, an increase in the levels of pro- and anti-inflammatory cytokines. We observed the most pronounced signs of the radiation-induced skin damage in the group of fractional irradiation after three months. Conclusion: 8 Gy and 40 Gy single local electron irradiation leads to a shift in the proliferative-apoptotic balance of keratinocytes towards their apoptosis, which activity is directly correlate with the dose of ionizing radiation, and 78 Gy in fractions leads to partial desquamation of the epithelium and inflammatory infiltration. In addition, after three months a significant increase in the expression of type I and type III collagen fibers and the development of radiation-induced skin fibrosis takes place against the background of 78 Gy fractional local electron irradiation.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical and histochemical analysis of the rat skin after local electron irradiation

Demyashkin,

Shapovalova,

Marukyan

et al. 2023

Open Vet J

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“…A decrease in the density of microvessels in the skin, leading to tissue ischemia and, thereby, significantly reducing the rate of wound healing after 25 Gy X-irradiation was found in another study. In addition, the authors noted an increase in the expression of pro-inflammatory cytokines (TNF-α, IL-1 and IL-6), the release of cell adhesion molecules, which accelerated the migration, adhesion and exudation of leukocytes and induced the migration of macrophages, neutrophils and monocytes, which leads to a local inflammatory response to radiation injury [26]. The polymorphonuclear infiltration of the skin found at various doses of single local electron irradiation can be explained by a similar mechanism the development of inflammation, the key role in which is played by the imbalance of pro-inflammatory and anti-inflammatory cytokines.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Analysis of the Skin After Local Electron Irradiation

Demyashkin,

Shapovalova,

Marukyan

et al. 2023

Preprint

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Skin cancer is the most frequently diagnosed type of cancer among all malignant neoplasms. The decrease in mitotic activity and the death of intact keratinocytes arise due to the constantly renewing epithelium is highly sensitive to ionising radiation. Objective: immunohistochemical evaluation of the proliferative-apoptotic balance of keratinocytes, the fibrous component of the skin and the expression of pro-inflammatory and anti-inflammatory cytokines after single or fractional local electron irradiation. Methods. Wistar rats (n=50) were taken and divided into groups: I – control (n=20), which were injected with saline; and experimental groups, local electron irradiation at doses: II – 8 Gy (n=10; single), III – 40 Gy (n=10, single), IV – summary dose 78 Gy (n=10; fractional; 13 Gy per day for 6 days). Results and Conclusions. 8 Gy and 40 Gy single local electron irradiation leads to a shift in the proliferative-apoptotic balance of keratinocytes towards their apoptosis, the activity of which is directly correlate with the dose of ionizing radiation, and 78 Gy summary dose in fractions leads to partial desquamation of the epithelium and inflammatory infiltration. In addition, a significant increase in the expression of type I and type III collagen fibers and the development of signs of radiation-induced skin fibrosis takes place against the background of 78 Gy fractional local electron irradiation. At the same time, after single 8 Gy and 40 Gy electron irradiation the described immunohistochemical changes were insignificant and directly correlated with the dose of ionizing radiation.

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“…В другом исследовании, после воздействия рентгеновского излучения в дозе 25 Гр было обнаружено уменьшение плотности микрососудов в коже, приводящее к ишемии тканей и, тем самым, значительно снижающее скорость заживления ран. Кроме того, авторы отмечали увеличение экспрессии провоспалительных факторов (TNF-α, IL-1 и IL-6) высвобождение молекул клеточной адгезии, что ускоряло миграцию, адгезию и экссудацию лейкоцитов и индуцировало миграцию макрофагов, нейтрофилов и моноцитов, как клеточного субстрата местной воспалительной реакции в ответ на лучевое поражение [18]. Вероятно, обнаруженная полиморфно-клеточная инфильтрация кожи при различных дозах облучения электронами может быть объяснена механизмом развития воспаления.…”

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Immunohistochemical Assessment of Proliferation and Apoptosis of Keratinocytes After a Single Fractional Ionizing Irradiation

Shapovalova,

Marukyan,

Vadyukhin

et al. 2023

MorphoLetter

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Radiation therapy for non-melanoma skin cancers is used by oncologists and radiologists in cases of ineffective surgical treatment and as adjuvant or palliative therapy. The creation of experimental models to study the proliferative-apoptotic balance of keratinocytes makes it possible to assess the degree and depth of post-radiation skin damage after exposure to electrons in order to select optimal doses in the clinic. The purpose of the study: immunohistochemical assessment of proliferation and apoptosis of keratinocytes after local electron irradiation of the skin. The experiment used male Wistar rats (n=50), which were divided into groups: I – control (n=20), which were injected with saline solution, and experimental rats, which received local electron irradiation of the skin of the outer surface of the thigh: II – 8 Gy (n=10; single), III – 40 Gy (n=10, single), IV – fractional 13 Gy for 6 days with a total focal dose of 78 Gy (n=10;). After completion of the experiment, a skin biopsy of the outer surface of the thigh was performed and an immunohistochemical study of histological preparations with antibodies to the Ki-67, caspase-3 and p53 antigens was performed. Analysis of skin fragments after irradiation showed a shift in the proliferative-apoptotic balance towards keratinocyte apoptosis: a decrease in the level of Ki-67 expression and an increase in the number of p53-positive cells. After a single irradiation with electrons at doses of 8 Gy and 40 Gy, a significant increase in keratinocytes stained positively with antibodies to caspase-3 was found - by 3.4 and 6.6 times, respectively, and with a fractional irradiation mode at a total dose of 78 Gy - by 11.6 times compared to the control group. Thus, local irradiation with electrons in single doses of 8 Gy and 40 Gy leads to a shift in the proliferative-apoptotic balance of keratinocytes towards their apoptosis, the activity of which is directly proportional to the dose of ionizing radiation, and the fractional mode (total focal dose of 78 Gy) leads to partial desquamation of the epithelium and inflammatory infiltration.

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Promoting repair of highly purified stromal vascular fraction gel combined with advanced platelet-rich fibrin extract for irradiated skin and soft tissue injury

Cited by 3 publications

References 43 publications

Immunohistochemical and histochemical analysis of the rat skin after local electron irradiation

Immunohistochemical and histochemical analysis of the rat skin after local electron irradiation

Immunohistochemical Analysis of the Skin After Local Electron Irradiation

Immunohistochemical Assessment of Proliferation and Apoptosis of Keratinocytes After a Single Fractional Ionizing Irradiation

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