Developing microorganisms with a high ribonucleic acid
(RNA) content
is crucial for the RNA industry. Numerous studies have been conducted
to enhance RNA production in yeast cells through genetic engineering,
yet precise mechanisms remain elusive. Previously, upregulation of TAL1 or PGM2 and deleting PRS5 or DBP8 individually could increase the RNA content
in Saccharomyces pastorianus. In this study, within
these genetically modified strains, the intracellular nucleotide levels
notably increased following cell fragmentation. Deletion of PRS5 and DBP8 within the strain prompted
the upregulation of genes sharing similar functions, consequently
augmenting the flow of the gene pathway. Furthermore, the upregulation
of genes encoding cell-cycle-dependent protein kinases (CDK) was observed
in the G03-△PRS5 strain. The influence of TAL1 and PGM2 on RNA content was attributed to the pentose
phosphate pathway (PPP). The RNA content of polygenic recombinant
strains, G03-△PRS5+△DBP8 and G03-△PRS5+△DBP8+PGM2,
displayed the most significant improvement, increasing by 71.8 and
80.1% when compared to the parental strain. Additionally, the maximum
specific growth rate of cells increased in these strains. This study
contributes valuable insights into the genetic mechanisms underlying
high nucleic acid synthesis in S. pastorianus.