Promoter variants at AP2 box region of Hsp70.1 affect thermal stress response and milk production traits in Frieswal cross bred cattle

Deb, Rajib; Sajjanar, Basavaraj; Singh, Umesh; Kumar, Sushil; Brahmane, M. P.; Singh, Rani; Sengar, Gyanendra Singh; Sharma, Arjava

doi:10.1016/j.gene.2013.09.037

Cited by 52 publications

(52 citation statements)

References 33 publications

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“…It has been reported that the polymorphisms occurring naturally in the functional promoter regions affect the expression of HSP70 and contribute to differential stress tolerance [16], [28], [34]. In the present study, a total of 11 SNP sites were found in the promoter region of AiHSP70, which changed the binding sites for certain transcription factors, and they might affect the transcriptional efficiency.…”

Section: Discussionmentioning

confidence: 53%

“…For example, the SNP 2437 T/C in the coding region of human HSPA1L is associated with heat shock response [15], and the −895 C insert-deletion (ins-del) in the promoter region of bovine HSP70.1 gene is associated with their summer heat tolerance by regulating the mRNA expression of HSP70.1 after heat stress [16]. In Chinese Holstein cattle, the haplotype 1 (TAATTACG) was associated with the heat-sensitive trait [17].…”

Section: Introductionmentioning

confidence: 99%

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The Polymorphism in the Promoter of HSP70 Gene Is Associated with Heat Tolerance of Two Congener Endemic Bay Scallops (Argopecten irradians irradians and A. i. concentricus)

Yang

Wang

et al. 2014

PLoS ONE

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BackgroundThe heat shock protein 70 (HSP70) is one kind of molecular chaperones, which plays a key role in protein metabolism under normal and stress conditions.MethodologyIn the present study, the mRNA expressions of HSP70 under normal physiological condition and after acute heat stress were investigated in gills of two bay scallop populations (Argopecten irradians irradians and A. i. concentricus). The heat resistant scallops A. i. concentricus showed significantly lower basal level and higher induction of HSP70 compared with that of the heat sensitive scallops A. i. irradians. The promoter sequence of HSP70 gene from bay scallop (AiHSP70) was cloned and the polymorphisms within this region were investigated to analyze their association with heat tolerance. Totally 11 single nucleotide polymorphisms (SNPs) were identified, and four of them (−967, −480, −408 and −83) were associated with heat tolerance after HWE analysis and association analysis. Based on the result of linkage disequilibrium analysis, the in vitro transcriptional activities of AiHSP70 promoters with different genotype were further determined, and the results showed that promoter from A. i. concentricus exhibited higher transcriptional activity than that from A. i. irradians (P<0.05).ConclusionsThe results provided insights into the molecular mechanisms underlying the thermal adaptation of different congener endemic bay scallops, which suggested that the increased heat tolerance of A. i. concentricus (compared with A. i. irradians) was associated with the higher expression of AiHSP70. Meanwhile, the −967 GG, −480 AA, −408 TT and −83 AG genotypes could be potential markers for scallop selection breeding with higher heat tolerance.

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Section: Discussionmentioning

confidence: 53%

Section: Introductionmentioning

confidence: 99%

The Polymorphism in the Promoter of HSP70 Gene Is Associated with Heat Tolerance of Two Congener Endemic Bay Scallops (Argopecten irradians irradians and A. i. concentricus)

Yang

Wang

et al. 2014

PLoS ONE

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“…Effects of the SNP g.1524G>A, g.3494T>C and g.6601G>A within HSP70A1A affect thermo-tolerance in Chinese Holstein cattle . In contrast allele T at SNP g.4338>C of HSP90AB1 gene was found to be associated with heat tolerance coefficient in Thai native cattle (Charoensook et al, 2013), Sahiwal and Frieswal cattle in India (Deb et al, 2013;Sajjanar et al, 2015). Recently in Jersey crossbred cows found that of CC genotype had better thermo-tolerance capacity at (T17872112C) locus of HSP90AB1 gene .…”

Section: Pcr Primers and Amplificationsmentioning

confidence: 93%

“…For example, polymorphisms detected in ATP1A gene at position "2789 and HSP70.1 at Position "895 were associated with the heat tolerance trait in dairy cattle (Liu et al, 2011;Deb et al, 2013). Effects of the SNP g.1524G>A, g.3494T>C and g.6601G>A within HSP70A1A affect thermo-tolerance in Chinese Holstein cattle .…”

Section: Pcr Primers and Amplificationsmentioning

confidence: 98%

Single nucleotide polymorphisms in Heat Shock Protein (HSP) 90AA1 gene and their association with heat tolerance traits in Sahiwal cows

Kumar

Gupta

Verma

et al. 2015

IJAR

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Heat Shock Proteins (HSPs) are group of proteins which are synthesized during heat stress. HSP genes have been reported to be associated with heat tolerance and production performance in cattle. HSP90AA1 gene has been mapped on Bos taurus autosome 21 (BTA 21) and spans nearly 5368 bp comprising 11 exons out of which first exon does not translate. The present study was carried out in Sahiwal cows (n=100) with the objectives to identify SNPs in targeted regions (exon 3, 7, 8 & 11) of HSP90AA1 gene and to analyze their association with heat tolerance traits in Sahiwal cows. Respiration rate (RR) and rectal temperature (RT) was recorded once during probable extreme hours in winter, spring and summer season. Further, heat tolerance coefficient (HTC) also calculated to see the adaptability of the animals during the period of heat stress. On the basis of comparative sequence analysis, total five SNPs were revealed at position of A1209G, A3292C, T4935C, T5218C and A5224C in the targeted region of HSP90AA1 gene. Out of these, only two SNPs at A1209G and A3292C loci were found significantly associated with heat tolerance traits in Sahiwal cows. Whereas, THI has a highly significant associated with RR, RT

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“…54 Though a generalized mechanism of stress-induced production of HSP is present in all cells, there will be difference in the capacity of individual animals to cope with the stress, along with the nucleotide changes occurring naturally in the flanking regions 5′-and 3′-untranslated region (UTR) of HSP gene resulting variation in inducibility, degree of expression, and/or stability of Hsp70 mRNA, which again contributes to different stress tolerance in individual animals. 69 There were also investigations done to study the association between SNPs in HSP70 in bovine 69 and swine. 70 Earlier there were also studies which revealed the association between SNPs in HSP with respiration rate and body temperature.…”

Section: Functions Of Hspsmentioning

confidence: 99%

Role of Heat Shock Proteins in Livestock Adaptation to Heat Stress

PR¹

2017

JDVAR

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The present review is an attempt to signify the importance of heat shock proteins in livestock adaptation during heat stress. The cellular and molecular responses in livestock are very crucial as it may lead to identification of confirmatory biomarker for heat stress in livestock. Thermo-tolerant gene expression and elevated heat shock protein (HSP) levels are observed to be the ultimate response through which the cell survives the heat stress. The HSPs have chaperonic activity ensuring the folding, unfolding and refolding of stress-denatured proteins. The components of heat shock response include heat shock factors (HSFs), heat shock element (HSE) and HSP. The cellular response to heat stress in mammalian organisms is controlled at the transcription level and it is mediated by a family of HSF which are regulated by the corresponding HSF genes. The activated HSFs bind with the HSE in the promoter region of HSP genes culminating in enhanced transcription of HSP mRNA. The HSP70, HSP90 and HSP27 are the predominant HSPs having protective role during heat stress in farm animals. Among these HSPs studied, HSP70 was identified to be the ideal biological marker for quantifying heat stress in animals.Keywords: heat stress, heat shock factors, heat shock response, hsp70, livestock Journal of Dairy, Veterinary & Animal Research Review Article Open AccessRole of heat shock proteins in livestock adaptation to heat stress the latter as it is harder to find an environment with a single variable changing. However, both these mechanisms are phenotypic responses as it is induced by the environment and the response goes once the stress is removed. It is usually produced to improve the fitness of the animal to the environment. Animals develop specific adaptive mechanisms if the environment stressors are present for a prolonged period. The mechanisms of adaptation include morphological, behavioural, biochemical and physiological changes. Biochemical adaptation to thermal stress involves changes in proteins, membrane lipids and metabolic rate.7 Genetic markers are of prior importance in the recent revolutionary developments in the field of molecular technology because of its viability as the biochemical adaptation can be utilized in livestock breeding programs compared to the behavioral, morphological or physiological responses to stress which is of less relevance. Heat shock protein is an important biomarker produced as cellular and tissue defense mechanism whose expression is markedly increased during heat shock. 8 The present review is an attempt to signify the importance of heat shock proteins in livestock adaptation during HS. General cellular responses to heat stressCellular exposure to thermal stress induces a number of anomalies in the functioning of cells which alters the biological molecules, disturbs cell functions, modulates metabolic reactions, induces oxidative cell damage and activates both apoptosis and necrosis pathways, ultimately leading to cell survival, acclimation or cell death depending on the time and...

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Promoter variants at AP2 box region of Hsp70.1 affect thermal stress response and milk production traits in Frieswal cross bred cattle

Cited by 52 publications

References 33 publications

The Polymorphism in the Promoter of HSP70 Gene Is Associated with Heat Tolerance of Two Congener Endemic Bay Scallops (Argopecten irradians irradians and A. i. concentricus)

The Polymorphism in the Promoter of HSP70 Gene Is Associated with Heat Tolerance of Two Congener Endemic Bay Scallops (Argopecten irradians irradians and A. i. concentricus)

Single nucleotide polymorphisms in Heat Shock Protein (HSP) 90AA1 gene and their association with heat tolerance traits in Sahiwal cows

Role of Heat Shock Proteins in Livestock Adaptation to Heat Stress

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