Promoter Structure and Activity of Type 1 Rice Metallothionein-like Gene

Hsieh, Hsing Mei; Huang, P. C.

doi:10.3109/10425179809050020

Cited by 8 publications

(5 citation statements)

References 33 publications

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“…However, the exact signaling pathways for transcriptional activation remain to be established. In contrast, the 5¢-upstream regions of MT genes have been isolated from several plant species, but studies of plant MT promoters have mainly been focused on examining the tissue expression of the MT genes (Fordham-Skelton et al 1997;Hsieh and Huang 1998;Guo et al 2003;Chatthai et al 2004;Fukuzawa et al 2004). To date, only a few plant MT promoters have been studied in detail.…”

Section: Introductionmentioning

confidence: 95%

The GUS reporter-aided analysis of the promoter activities of a rice metallothionein gene reveals different regulatory regions responsible for tissue-specific and inducible expression in transgenic Arabidopsis

Lü

Yuan

et al. 2006

Transgenic Res

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To gain a better understanding of the regulatory mechanism of plant metallothionein (MT) genes, a chimeric expression unit consisting of the beta-glucuronidase (gusA) reporter gene under the control of a 1,324 bp fragment of the rice MT (ricMT) promoter was introduced into Arabidopsis via Agrobacterium tumefaciens. The strongest histochemical staining for GUS activity was observed in the cotyledons and hypocotyls of the transgenic seedlings and in the stigma, filaments and anthers of young and mature flowers, and especially in the wounded tissues of transgenic plants. In contrast, a relatively low level of reporter gene expression was seen in the young roots of transgenic seedlings and no GUS activity was detected in the stems, seeds and leaves, but GUS activity was observed in cotyledons and the first two true leaves. Promoter analysis of 5' deletions further identified several important regions responsible for organ-specific expression including roots, flowers and wound induction, light and ABA, Cu and Zn responses. These findings demonstrate that a 1,324 bp fragment of the rice MT promoter performs a complicated transcriptional regulation with clearly functional regions in a model plant, and provide an important insight into the transcriptional regulation mechanisms that operate the temporal- and spatial-specific expression and stress responses of the rice MT gene. These results suggest that the ricMT promoter and its functional regions are potentially useful in genetic engineering of plants to express the desired genes whose products are preferentially needed in roots, flowers and wound induction.

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Section: Introductionmentioning

confidence: 95%

The GUS reporter-aided analysis of the promoter activities of a rice metallothionein gene reveals different regulatory regions responsible for tissue-specific and inducible expression in transgenic Arabidopsis

Lü

Yuan

et al. 2006

Transgenic Res

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“…Several studies have focused on the tissue-specificity of plant MT promoters (Fordham-Skelton et al 1997;Hsieh and Huang 1998;Chatthai et al 2004). For instance, 5 0 deletion analysis of the pea PsMTA promoter in transgenic Arabidopsis showed that the -583/-285 nt region was responsible for GUS expression in roots (Fordham-Skelton et al 1997).…”

Section: Introductionmentioning

confidence: 99%

“…Plant MTs are further divided into four types based on the distribution and arrangement of their cysteine residues (Robinson et al 1993;Cobbett and Goldsbrough 2002). Type 1 MTs are primarily expressed in plant roots and leaves (Foley et al 1997;Hsieh and Huang 1998), while type 2 MTs are expressed abundantly in leaves and roots of mature plants (Hsieh et al 1996). Type 3 MT transcripts are mainly found in developing fruits of plants such as banana, kiwi, apple, oil palm and mandarin (Ledger and Gardner 1994;Clendennen and May 1997;Reid and Ross 1997;Siti Nor Akmar et al 2002;Endo et al 2007), even though there are reports of their occurrence in leaves (Bratic et al 2009), and embryos (Dong and Dunstan 1996).…”

Section: Introductionmentioning

confidence: 99%

The oil palm metallothionein promoter contains a novel AGTTAGG motif conferring its fruit-specific expression and is inducible by abiotic factors

Omidvar

Abdullah

Izadfard

et al. 2010

Planta

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The 1,053-bp promoter of the oil palm metallothionein gene (so-called MSP1) and its 5' deletions were fused to the GUS reporter gene, and analysed in transiently transformed oil palm tissues. The full length promoter showed sevenfold higher activity in the mesocarp than in leaves and 1.5-fold more activity than the CaMV35S promoter in the mesocarp. The 1,053-bp region containing the 5' untranslated region (UTR) gave the highest activity in the mesocarp, while the 148-bp region was required for minimal promoter activity. Two positive regulatory regions were identified at nucleotides (nt) -953 to -619 and -420 to -256 regions. Fine-tune deletion of the -619 to -420 nt region led to the identification of a 21-bp negative regulatory sequence in the -598 to -577 nt region, which is involved in mesocarp-specific expression. Gel mobility shift assay revealed a strong interaction of the leaf nuclear extract with the 21-bp region. An AGTTAGG core-sequence within this region was identified as a novel negative regulatory element controlling fruit-specificity of the MSP1 promoter. Abscisic acid (ABA) and copper (Cu(2+)) induced the activity of the promoter and its 5' deletions more effectively than methyl jasmonate (MeJa) and ethylene. In the mesocarp, the full length promoter showed stronger inducibility in response to ABA and Cu(2+) than its 5' deletions, while in leaves, the -420 nt fragment was the most inducible by ABA and Cu(2+). These results suggest that the MSP1 promoter and its regulatory regions are potentially useful for engineering fruit-specific and inducible gene expression in oil palm.

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“…MT isoforms with different expression patterns may be transcriptionally regulated in different manners and have specialized functions in the corresponding tissues. To study the transcriptional regulation of each MT gene further, the 5′ promoter regions have also been isolated and studied to reveal their tissue‐specificity (Chatthai et al 1997; Fordham‐Skelton et al 1997; Hsieh and Huang 1998; Guo et al 2003; Fukuzawa et al 2004). For example, in the pea PsMTA promoter, the region −583/−285 is responsible for the expression of the downstream GUS reporter in the root of transgenic Arabidopsis (Fordham‐Skelton et al 1997).…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Rice Metallothionein Gene Promoter: Its Tissue Specificity and Heavy Metal Responsiveness

Dong¹,

Wang²,

Yu³

et al. 2010

JIPB

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The rice (Oryza sativa L.) metallothionein gene OsMT-I-4b has previously been identified as a type I MT gene. To elucidate the regulatory mechanism involved in its tissue specificity and abiotic induction, we isolated a 1 730 bp fragment of the OsMT-I-4b promoter region. Histochemical β-glucuronidase (GUS) staining indicated a precise spacial and temporal expression pattern in transgenic Arabidopsis. Higher GUS activity was detected in the roots and the buds of flower stigmas, and relatively lower GUS staining in the shoots was restricted to the trichomes and hydathodes of leaves. No activity was observed in the stems and seeds. Additionally, in the root of transgenic plants, the promoter activity was highly upregulated by various environmental signals, such as abscisic acid, drought, dark, and heavy metals including Cu 2+ , Zn 2+ , Pb 2+ and Al 3+ . Slight induction was observed in transgenic seedlings under salinity stress, or when treated with Co 2+ and Cd 2+ . Promoter analysis of 5 -deletions revealed that the region −583/−1 was sufficient to drive strong GUS expression in the roots but not in the shoots. Furthermore, deletion analysis indicated important promoter regions containing different metalresponsive cis-elements that were responsible for responding to different heavy metals. Collectively, these findings provided important insight into the transcriptional regulation mechanisms of the OsMT-I4b promoter, and the results also gave us some implications for the potential application of this promoter in plant genetic engineering.

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Promoter Structure and Activity of Type 1 Rice Metallothionein-like Gene

Cited by 8 publications

References 33 publications

The GUS reporter-aided analysis of the promoter activities of a rice metallothionein gene reveals different regulatory regions responsible for tissue-specific and inducible expression in transgenic Arabidopsis

The GUS reporter-aided analysis of the promoter activities of a rice metallothionein gene reveals different regulatory regions responsible for tissue-specific and inducible expression in transgenic Arabidopsis

The oil palm metallothionein promoter contains a novel AGTTAGG motif conferring its fruit-specific expression and is inducible by abiotic factors

Characterization of a Novel Rice Metallothionein Gene Promoter: Its Tissue Specificity and Heavy Metal Responsiveness

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