Promoter hypomethylation of RAR-related orphan receptor α 1 is correlated with unfavorable clinicopathological features in patients with colorectal cancer

Kano, Hisao; Takayama, Tadatoshi; Midorikawa, Yutaka; Nagase, Hiroki

doi:10.5582/bst.2016.01097

Cited by 13 publications

(9 citation statements)

References 26 publications

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“…Fu et al disclosed that RORα expression was downregulated in hepatocellular cancer (HCC) and concerned with serum AFP, pathology grade, tumor recurrence, invasion, and prognosis in HCC (10). Kano et al revealed that RORα1 expression was downregulated in colorectal cancer and cells; moreover, hypomethylation of the RORα1 promoter was correlated with TNM stage (14). Wang et al found that RORα expression was reduced in GC and associated with tumor size, differentiation, T stage, TNM stage, and lymph node metastasis (9).…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: RORα Suppresses Epithelial-to-Mesenchymal Transition and Invasion in Human Gastric Cancer Cells via the Wnt/β-Catenin Pathway

Xia

et al. 2019

Front. Oncol.

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Retinoid-related orphan receptor alpha (RORα) is involved in tumor development. However, the mechanisms underlying RORα inhibiting epithelial-to-mesenchymal transition (EMT) and invasion are poorly understood in gastric cancer (GC). This study revealed that the decreased expression of RORα is associated with GC development, progression, and prognosis. RORα suppressed cell proliferation, EMT, and invasion in GC cells through inhibition of the Wnt/β-catenin pathway. RORα overexpression resulted in the decreased Wnt1 expression and the increased RORα interaction with β-catenin, which could lead to the decreased intranuclear β-catenin and p-β-catenin levels, concomitant with downregulated T-cell factor-4 (TCF-4) expression and the promoter activity of c-Myc. The inhibition of Wnt/β-catenin pathway was coupled with the reduced expression of Axin, c-Myc, and c-Jun. RORα downregulated vimentin and Snail and upregulated E-cadherin protein levels in vitro and in vivo. Inversely, knockdown of RORα attenuated its inhibitory effects on Wnt/β-catenin pathway and its downstream gene expression, facilitating cell proliferation, EMT, migration, and invasion in GC cells. Therefore, RORα could play a crucial role in repressing GC cell proliferation, EMT, and invasion via downregulating Wnt/β-catenin pathway.

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Section: Discussionmentioning

confidence: 99%

RETRACTED: RORα Suppresses Epithelial-to-Mesenchymal Transition and Invasion in Human Gastric Cancer Cells via the Wnt/β-Catenin Pathway

Xia

et al. 2019

Front. Oncol.

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“…As for RAP1B and RAP2A, RAP1B has been confirmed to be specifically activated in nerve system and contributes to the regeneration of neuronal connectivity, the dysfunction of which turns out to be one of the pathological factors for epilepsy, validating the regulatory role of RAP1B during such disease [ 96 ]. RAP2A has been validated and confirmed to contribute to the childhood absence epilepsy, a specific subtype of epilepsy, and may be related with a specific glioma inducing epilepsy associated symptoms, validating our prediction of epilepsy associated genes [ 97 , 98 ]. As for RRAS and MRAS, considering the functional similarity of MRAS and MAPK, the detailed analysis of such genes can be seen below, while the inner relationship between RRAS and epilepsy has also been revealed in mouse model, validating our prediction [ 99 , 100 ].…”

Section: Discussionmentioning

confidence: 68%

Identifying and Analyzing Novel Epilepsy-Related Genes Using Random Walk with Restart Algorithm

Guo

Shang

Cao

et al. 2017

BioMed Research International

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As a pathological condition, epilepsy is caused by abnormal neuronal discharge in brain which will temporarily disrupt the cerebral functions. Epilepsy is a chronic disease which occurs in all ages and would seriously affect patients' personal lives. Thus, it is highly required to develop effective medicines or instruments to treat the disease. Identifying epilepsy-related genes is essential in order to understand and treat the disease because the corresponding proteins encoded by the epilepsy-related genes are candidates of the potential drug targets. In this study, a pioneering computational workflow was proposed to predict novel epilepsy-related genes using the random walk with restart (RWR) algorithm. As reported in the literature RWR algorithm often produces a number of false positive genes, and in this study a permutation test and functional association tests were implemented to filter the genes identified by RWR algorithm, which greatly reduce the number of suspected genes and result in only thirty-three novel epilepsy genes. Finally, these novel genes were analyzed based upon some recently published literatures. Our findings implicate that all novel genes were closely related to epilepsy. It is believed that the proposed workflow can also be applied to identify genes related to other diseases and deepen our understanding of the mechanisms of these diseases.

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“…Although four isoforms of RORA (RORA1ÀRORA4) can be produced according to their different N-termini [39] in normal human tissues, RORA1 and RORA4 are the main two isoforms transcribed [14]. RORA1, rather than RORA4, is mainly expressed in the central nervous system and is correlated with unfavorable clinicopathological features of colorectal cancer patients [39,40]. Therefore, isoform RORA1 was chosen in this study.…”

Section: Discussionmentioning

confidence: 99%

MiR-18a-downregulated RORA inhibits the proliferation and tumorigenesis of glioma using the TNF-α-mediated NF-κB signaling pathway

Jiang¹,

Zhou

Zhao

et al. 2020

EBioMedicine

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Background: Glioma has a poor prognosis, and is the most common primary and lethal primary malignant tumor in the central nervous system. Retinoic acid receptor-related orphan receptor A (RORA) is a member of the ROR subfamily of orphan receptors and plays an anti-tumor role in several cancers. Methods: A cell viability assay, the Edu assay, neurosphere formation assay, and xenograft experiments were used to detect the proliferative abilities of glioma cell line, glioma stem cells (GSCs). Western blotting, ELISAs, and luciferase reporter assays were used to detect the presence of possible microRNAs. Findings: Our study found for the first time that RORA was expressed at low levels in gliomas, and was associated with a good prognosis. RORA overexpression inhibited the proliferation and tumorigenesis of glioma cell lines and GSCs via inhibiting the TNF-a mediated NF-kB signaling pathway. In addition, microRNA-18a had a promoting effect on gliomas, and was the possible reason for low RORA expression in gliomas. Interpretation: RORA may be a promising therapeutic target in the treatment of gliomas.

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Promoter hypomethylation of RAR-related orphan receptor α 1 is correlated with unfavorable clinicopathological features in patients with colorectal cancer

Cited by 13 publications

References 26 publications

RETRACTED: RORα Suppresses Epithelial-to-Mesenchymal Transition and Invasion in Human Gastric Cancer Cells via the Wnt/β-Catenin Pathway

RETRACTED: RORα Suppresses Epithelial-to-Mesenchymal Transition and Invasion in Human Gastric Cancer Cells via the Wnt/β-Catenin Pathway

Identifying and Analyzing Novel Epilepsy-Related Genes Using Random Walk with Restart Algorithm

MiR-18a-downregulated RORA inhibits the proliferation and tumorigenesis of glioma using the TNF-α-mediated NF-κB signaling pathway

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