Promoter hypermethylation in circulating blood cells identifies prostate cancer progression

Abstract

Promoter hypermethylation of circulating cell DNA has been advocated as a diagnostic marker for prostate cancer, but its prognostic use is currently unclear. To assess this role, we compared hypermethylation of circulating cell DNA from prostate cancer patients with (Group 1, n = 20) and without (Group 2, n = 22) disease progression and age‐matched controls (benign prostatic hyperplasia, Group 3, n = 22). We measured hypermethylation of 10 gene promoters in 2 sequential venous samples, obtained at diagnosis and during disease progression (median time, 15 months later). Matched time samples were obtained in the nonprogressing patients. We found that more hypermethylation was detected in the diagnostic sample from the patients with cancer than in controls for GSTP1, RASSF1a, APC and RARβ (p < 0.0001). Patients undergoing disease progression had a significant increase in methylation levels of these 4 genes when compared to the other patients (p < 0.001). Patients at risk of disease progression have higher detectable concentrations of circulating cell hypermethylation, than those without progression. The extent of this hypermethylation increases during disease progression and can be used to identify the extent and duration of treatment response in prostate cancer. © 2007 Wiley‐Liss, Inc.

DNA Methylation Changes Correlate with Gleason Score and Tumor Stage in Prostate Cancer

“…The selected loci were those previously shown to discriminate presence of PCa in cases when compared with controls using urine, plasma, and serum samples (Hoque et al, 2005;Roupret et al, 2007Roupret et al, , 2008Li, 2007;Baden et al, 2009;Hoque, 2009;Phe et al, 2010). Our data included paired tumor, nontumor tissues, and plasma samples from cases, which allowed us to investigate not only tumor/nontumor differences, but also the tissue-plasma correlations.…”

Evaluation of Extracted Circulating Cell free DNA Concentration by Standard Nucleospin Plasma XS (NS) Kit Protocol Compared to its Modified Protocol

“…The amount of circulating DNA in blood serum is very low and different in people based on their health condition. For example, concentrations in healthy subjects is 20-30 ng/ml and in men with metastatic prostate cancer is close to 40 ng/ml and almost 1200 ng/ml in patients with other types of cancer [2]. However, when NanoDrop, PCR and Real-time-PCR were used to evaluate the concentrations of cell free DNA, we noticed that the extracts did not meet our expectation as was written in the kit manual.…”

APC gene hypermethylation and prostate cancer: a systematic review and meta-analysis

“…Our search strategy and application of the inclusion/exclusion criteria resulted in a total of 19 articles that were included in the systematic review. [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] A description of the included studies is given in Tables 1 and 2. The following data were recorded for each study: author's name, year of publication, sample forms, method, 5 0 -3 0 primers (forward and reverse, respectively), amplicon size (bp) and annealing temperature (1C), country, race, cancer clinical classification, PSA, Gleason score, type of cases and controls, type of PCR method and other relevant characteristics of the study population.…”

“…[39][40][41][42][43][44][45][46][47][48][49][50][51][52][53] Finally, 19 studies met the inclusion criteria and were included. [11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] Among these 19 studies, 5 involved body fluid (blood, urine and so on) [11][12][13][14][15] and the remaining 14 articles involved sample tissues. [16][17][18][19][20][21][22][23][24][25][26][27]…”

“…[11][12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29] Among these 19 studies, 5 involved body fluid (blood, urine and so on) [11][12][13][14][15] and the remaining 14 articles involved sample tissues. [16][17][18][19][20][21][22][23][24][25][26][27][28][29] Ten studies used quantitative real-time methylation-specific PCR (QMSP) to detect APC methylation, 11,13,14,18,19,[21][22][23]25,26 seven were conducted using the method of methylation-specific PCR (MSP), 12,15,...…”