Promoter engineering for microbial bio-alkane gas production

Trisrivirat, Duangthip; Hughes, Juliette H.; Hoeven, Robin; Faulkner, Matthew; Toogood, Helen S.; Chaiyen, Pimchai; Scrutton, Nigel S.

doi:10.1093/synbio/ysaa022

Cited by 6 publications

(13 citation statements)

References 35 publications

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“…A new class of photoenzyme, fatty acid photodecarboxylase, has recently been discovered that may allow for improved anaerobic digestion by the light-powered conversion of organic acids into hydrocarbon gases [ 88 ]. Fatty acid photodecarboxylases have now been shown to be present in a wide variety of algal species [ 89 ] and have also been the subject of advanced biochemical and genetic studies [ 90 , 91 ]. For this application, live microalgae cells are not used, rather photodecarboxylase enzyme derived from microalgae is used.…”

Section: Can Photodecarboxylase Be a Catalyst For Change?mentioning

confidence: 99%

Shining a Light on Wastewater Treatment with Microalgae

Kilbane

2022

Arab J Sci Eng

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Microalgae can produce biofuels, nutriceuticals, pigments and many other products, but commercialization has been limited by the cost of growing, harvesting and processing algal biomass. Nutrients, chiefly nitrogen and phosphorus, are a key cost for growing microalgae, but these nutrients are present in abundance in municipal wastewater where they pose environmental problems if not removed. This is not a traditional review article; rather, it is a fact-based set of suggestions that will have to be investigated by scientists and engineers. It is suggested that if microalgae were grown as biofilms rather than as planktonic cells, and if internal illumination rather than external illumination were employed, then the use of microalgae may provide useful improvements to the wastewater treatment process. The use of microalgae to remove nutrients from wastewater has been demonstrated, but has not yet been widely implemented due to cost, and because microalgae derived from wastewater treatment has not yet been demonstrated as a commercial source for value-added products. Future facilities are likely to be called Municipal Resource Recovery Facilities as wastewater will increasingly be viewed as a resource for water, biofuels, fertilizer, monitoring public health and value-added products. Advances in photonics will accelerate this transition.

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Section: Can Photodecarboxylase Be a Catalyst For Change?mentioning

confidence: 99%

Shining a Light on Wastewater Treatment with Microalgae

Kilbane

2022

Arab J Sci Eng

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“…The content in this section focuses on enzymatic and metabolically engineered cells for production of hydrocarbons and fatty alcohol as value-added products from fatty acids. Alkanes and alkenes are hydrocarbon organic materials mainly found in fossil fuels . They are major components in natural gas and petroleum, and are used as fuels for combustion in engines, as monomers for polymer production, and as solvents and plastics.…”

Section: Production Of Triglyceride- Fatty Acid- and Glycerol-derived...mentioning

confidence: 99%

Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability

et al. 2021

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Since the industrial revolution, the rapid growth and development of global industries have depended largely upon the utilization of coal-derived chemicals, and more recently, the utilization of petroleum-based chemicals. These developments have followed a linear economy model (produce, consume, and dispose). As the world is facing a serious threat from the climate change crisis, a more sustainable solution for manufacturing, i.e., circular economy in which waste from the same or different industries can be used as feedstocks or resources for production offers an attractive industrial/business model. In nature, biological systems, i.e., microorganisms routinely use their enzymes and metabolic pathways to convert organic and inorganic wastes to synthesize biochemicals and energy required for their growth. Therefore, an understanding of how selected enzymes convert biobased feedstocks into special (bio)chemicals serves as an important basis from which to build on for applications in biocatalysis, metabolic engineering, and synthetic biology to enable biobased processes that are greener and cleaner for the environment. This review article highlights the current state of knowledge regarding the enzymatic reactions used in converting biobased wastes (lignocellulosic biomass, sugar, phenolic acid, triglyceride, fatty acid, and glycerol) and greenhouse gases (CO 2 and CH 4 ) into value-added products and discusses the current progress made in their metabolic engineering. The commercial aspects and life cycle assessment of products from enzymatic and metabolic engineering are also discussed. Continued development in the field of metabolic engineering would offer diversified solutions which are sustainable and renewable for manufacturing valuable chemicals.

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“…( 2018 ) created new strong promoters for usage in L. citreum using the semi‐artificial approach, while two other studies used semi‐artificial sequences to create constitutive and inducible promoters in the non‐model organism Halomonas sp. (Li et al ., 2016 ; Trisrivirat et al ., 2020 ). In the study by Trisrivirat et al .…”

Section: Context Dependency Challengementioning

confidence: 99%

“…In the study by Trisrivirat et al . ( 2020 ), a Halomonas sp. was used to produce propane with the novel semi‐artificial promoter that they created.…”

Section: Context Dependency Challengementioning

confidence: 99%

Importance of the 5′ regulatory region to bacterial synthetic biology applications

Tietze

Lale

2021

Microbial Biotechnology

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The field of synthetic biology is evolving at a fast pace. It is advancing beyond single-gene alterations in single hosts to the logical design of complex circuits and the development of integrated synthetic genomes. Recent breakthroughs in deep learning, which is increasingly used in de novo assembly of DNA components with predictable effects, are also aiding the discipline. Despite advances in computing, the field is still reliant on the availability of precharacterized DNA parts, whether natural or synthetic, to regulate gene expression in bacteria and make valuable compounds. In this review, we discuss the different bacterial synthetic biology methodologies employed in the creation of 5 0 regulatory regionspromoters, untranslated regions and 5 0 -end of coding sequences. We summarize methodologies and discuss their significance for each of the functional DNA components, and highlight the key advances made in bacterial engineering by concentrating on their flaws and strengths. We end the review by outlining the issues that the discipline may face in the near future.

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Promoter engineering for microbial bio-alkane gas production

Cited by 6 publications

References 35 publications

Shining a Light on Wastewater Treatment with Microalgae

Shining a Light on Wastewater Treatment with Microalgae

Enzymes, In Vivo Biocatalysis, and Metabolic Engineering for Enabling a Circular Economy and Sustainability

Importance of the 5′ regulatory region to bacterial synthetic biology applications

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