Promoter and neck region length variation of DC-SIGN is not associated with susceptibility to tuberculosis in Tunisian patients

Ben-Ali, Meriem; Barreiro, Luis B.; Chabbou, A.; Haltiti, Raja; Braham, Ezzeddine; Neyrolles, Olivier; Dellagi, Koussay; Gicquel, Brigitte; Quintana-Murci, Lluı́s; Barbouche, Mohamed‐Ridha

doi:10.1016/j.humimm.2007.09.003

Cited by 42 publications

(35 citation statements)

References 18 publications

(23 reference statements)

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“…The frequency of SNP -336A/G in some African regions, such as in South Africa (40.3%) and Guinea-Bissau (49.9%), is considerably higher than the frequency found in this study (P < 0.05) (Barreiro et al, 2006;Olesen et al, 2007). However, in some northern African countries, such as Tunisia and Morocco, the frequency of this polymorphism is not statistically different from that of European countries (Ben-Ali et al, 2007;Sadki et al, 2009). Moreover, the frequency of allele -336G observed in this study was significantly higher than the frequency observed in Asian populations, such as those of eastern China (7.3%) (Zheng et al, 2011), northern Thailand (9.5%) (Wichukchinda et al, 2007), and different regions of Taiwan (Table 2).…”

Section: Discussioncontrasting

confidence: 83%

Frequency of SNP -336A/G in the promoter region of CD209 in a population from northeastern Brazil

Pn¹,

Ferreira-Fernandes²,

Js³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Dendritic cells (DCs) mediate the initiation of the immune response against a variety of pathogens. The DC-SIGN receptor is encoded by the gene CD209 and is expressed on the surface of DCs. It binds to mannose-rich carbohydrates and enables the recognition of bacteria, fungi, parasites, and viruses. SNP -336A/G in the promoter region of CD209 influences the expression of the DC-SIGN receptor. Several studies have associated this SNP with an increased susceptibility to infectious diseases and the development of more severe forms of disease. Therefore, the aim of this study was to determine the prevalence of SNP -336A/G in a population from northeastern Brazil. We analyzed 181 individuals from the general population of Parnaíba, Piauí, Brazil, of which 37% were men and 63% were women. SNP -336A/G was detected by polymerase chain reaction and treatment with the restriction enzyme MscI and visualized by electrophoresis on an 8% polyacrylamide gel stained with silver nitrate. Of the individuals analyzed, 116 (64.1%) were homozygous AA, 57 (31.5%) were heterozygous (AG), and 8 (4.4%) were homozygous GG. The allele frequency of -336G was 20.2%. Genotype frequencies were in Hardy-Weinberg equilibrium. To the best of our knowledge, this is the first report to describe the frequency of the CD209 SNP -336A/G in a population in the State of Piauí. Further studies are needed to determine the relationship between this SNP and the vulnerability of this population to major infectious diseases.

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Section: Discussioncontrasting

confidence: 83%

Frequency of SNP -336A/G in the promoter region of CD209 in a population from northeastern Brazil

Pn¹,

Ferreira-Fernandes²,

Js³

et al. 2015

Genet. Mol. Res.

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“…Thirteen of the 14 included studies clearly described the diagnostic criteria. One study didn’t provide the genotype number [18]. Gene frequencies of all the individual studies were shown in Table S1.…”

Section: Resultsmentioning

confidence: 99%

Association between CD209 -336A/G and -871A/G Polymorphisms and Susceptibility of Tuberculosis: A Meta-Analysis

Chang

Deng

et al. 2012

PLoS ONE

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BackgroundThe association between CD209 promoter polymorphisms (-336A/G, -871A/G) and tuberculosis (TB) risk has been widely reported, but results of previous studies remain controversial and ambiguous. To assess the association between CD209 polymorphisms and TB risk, a meta-analysis was performed.MethodsBased on comprehensive searches of the PubMed, Embase, Web of Science, Weipu, and CBM databases, we identified outcome data from all articles estimating the association between CD209 polymorphisms and TB risk. The pooled odds ratio (OR) with 95% confidence intervals (CIs) were calculated.ResultsA total of 14 studies with 3,610 cases and 3,539 controls were identified. There was no significant association between CD209 -336A/G polymorphism and TB risk (OR = 1.04, 95% CI = 0.91–1.19 for G vs. A; OR = 1.13, 95% CI = 0.84–1.53 for GG vs. AA; OR = 1.04, 95% CI = 0.87–1.24 for GG+AG vs. AA; OR = 1.11, 95% CI = 0.88–1.39 for GG vs. AG+AA). However, the significant association was revealed for Asians in GG vs. AA (OR = 2.48, 95% CI = 1.46–4.22, P = 0.0008) and GG vs. AG+AA (OR = 2.10, 95% CI = 1.33–3.32, P = 0.001). For the CD209 -871A/G polymorphism, lack of an association was also found (OR = 0.81, 95% CI = 0.70–0.95 for G vs. A; OR = 1.00, 95% CI = 0.52–1.93 for GG vs. AA; OR = 0.73, 95% CI = 0.60–0.89 for GG+AG vs. AA; OR = 1.09, 95% CI = 0.57–2.10 for GG vs. AG+AA).ConclusionThe present meta-analysis suggested that CD209 promoter polymorphisms (-336A/G, -871A/G) were unlikely to substantially contribute to TB susceptibility. However, the GG genotype of CD209 -336A/G polymorphism might be a genetic risk factor that increases TB susceptibility for Asians in GG vs. AA and GG vs. AG+AA.

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“…However, studies investigating single nucleotide polymorphisms in the promoter region of DC-SIGN have shown a reduced risk of TB associated with both downregulation (70) and upregulation (7) of DC-SIGN expression or even no association with susceptibility to TB at all (9,29). A study with mice lacking SIGNR1-one of the murine homologs of DC-SIGNshowed that these mice had stronger T-helper 1 responses to M. tuberculosis and a reduced level of IL-10 (72).…”

Section: Discussionmentioning

confidence: 99%

Role of Phosphatidylinositol Mannosides in the Interaction between Mycobacteria and DC-SIGN

et al. 2009

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The C-type lectin dendritic cell (DC)-specific intercellular adhesion molecule 3-grabbing nonintegrin (DC-SIGN) is the major receptor on DCs for mycobacteria of the Mycobacterium tuberculosis complex. Recently, we have shown that although the mannose caps of the mycobacterial surface glycolipid lipoarabinomannan (ManLAM) are essential for the binding to DC-SIGN, genetic removal of these caps did not diminish the interaction of whole mycobacteria with DC-SIGN and DCs. Here we investigated the role of the structurally related glycolipids phosphatidylinositol mannosides (PIMs) as possible ligands for DC-SIGN. In a binding assay with both synthetic and natural PIMs, DC-SIGN exhibited a high affinity for hexamannosylated PIM 6 , which contains terminal ␣(132)-linked mannosyl residues identical to the mannose cap on ManLAM, but not for di-and tetramannosylated PIM 2 and PIM 4 , respectively. To determine the role of PIM 6 in the binding of whole mycobacteria to DC-SIGN, a mutant strain of M. bovis bacillus Calmette-Guérin deficient in the production of PIM 6 (⌬pimE) was created, as well as a double knockout deficient in the production of both PIM 6 and the mannose caps on LAM (⌬pimE ⌬capA). Compared to the wild-type strain, both mutant strains bound similarly well to DC-SIGN and DCs. Furthermore, the wild-type and mutant strains induced comparable levels of interleukin-10 and interleukin-12p40 when used to stimulate DCs. Hence, we conclude that, like ManLAM, PIM 6 represents a bona fide DC-SIGN ligand but that other, as-yet-unknown, ligands dominate in the interaction between mycobacteria and DCs.

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Promoter and neck region length variation of DC-SIGN is not associated with susceptibility to tuberculosis in Tunisian patients

Cited by 42 publications

References 18 publications

Frequency of SNP -336A/G in the promoter region of CD209 in a population from northeastern Brazil

Frequency of SNP -336A/G in the promoter region of CD209 in a population from northeastern Brazil

Association between CD209 -336A/G and -871A/G Polymorphisms and Susceptibility of Tuberculosis: A Meta-Analysis

Role of Phosphatidylinositol Mannosides in the Interaction between Mycobacteria and DC-SIGN

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