Promoter analysis of a soybean nuclear gene coding for nodulin-23, a nodule-specific polypeptide involved in symbiosis with <i>Rhizobium</i>

Wong, Sandy; Verma, Desh Pal S.

doi:10.1002/j.1460-2075.1985.tb03952.x

Cited by 12 publications

(8 citation statements)

References 36 publications

(50 reference statements)

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“…RNAse protection experiments were carried out according to Coruzzi et aI. [10], and the position of the transcription start sites was determined as described by Wong and Verma [50].…”

Section: Clone Namesmentioning

confidence: 99%

Identification of several soybean cytosolic glutamine synthetase transcripts highly or specifically expressed in nodules: expression studies using one of the corresponding genes in transgenic Lotus corniculatus

1995

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A DNA fragment containing sequences hybridizing to the 5' region of GS15, a gene encoding soybean cytosolic glutamine synthetase, was isolated from a soybean genomic library. Mapping and partial sequence analysis of the genomic clone revealed that it encodes a cytosolic GS gene, GS21, which is different from GS15. In parallel, a number of cDNA clones encoding cytosolic GS were isolated using the coding region of pGS20 as a probe (pGS20 is a cDNA clone which corresponds to a transcript of the GS15 gene). Two new full-length cDNAs designated pGS34 and pGS38 were isolated and sequenced. In the 5' non-coding region a strong homology was found between the two clones and the GS21 gene. However, none of these sequences were identical, which suggests that there are at least three members in this group of genes. In order to determine their relative levels of transcription, specific sequences from pGS34, pGS38 and GS21 were used in an RNAse protection assay. This experiment clearly showed that GS21 and the gene encoding pGS38 are specifically expressed in young or mature nodules, whereas the gene encoding pGS34 is highly transcribed in nodules and constitutively expressed at a lower level in other soybean organs. In order to further analyse the molecular mechanisms controlling GS21 transcription, different fragments of the promoter region were fused to the Escherichia coli reporter gene encoding beta-glucuronidase (GUS) and the constructs were introduced into Lotus corniculatus via Agrobacterium rhizogenes-mediated transformation. Analysis of GUS activity showed that the GS21 promoter-GUS constructs were expressed in the vasculature of all vegetative organs. This result is discussed in relation to species-specific metabolic and developmental characteristics of soybean and Lotus.

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“…RNAse protection experiments were carried out according to Coruzzi et aI. [10], and the position of the transcription start sites was determined as described by Wong and Verma [50].…”

Section: Clone Namesmentioning

confidence: 99%

Identification of several soybean cytosolic glutamine synthetase transcripts highly or specifically expressed in nodules: expression studies using one of the corresponding genes in transgenic Lotus corniculatus

1995

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“…The nodulin consensus motifs 5'-CTCTT and 5'-AAAGAT are underlined with one bar; lnvA and lnvB are underlined with two bars. Positions are indicated relative to the major cap site of the original N23 promoter (Wong and Verma, 1985).…”

Section: Definition Of Pe-amentioning

confidence: 99%

“…The Mutated sequences are shown below the original sequences. Positions are indicated with respect to the major cap site of the original N23 promoter (Wong and Verma, 1985). Underlining indicates the single mismatch between lnvA and InvB.…”

Section: Point Mutations In Lnva and Invbmentioning

confidence: 99%

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A two-component nodule-specific enhancer in the soybean N23 gene promoter.

1991

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The two positive cis elements in the soybean nodulin N23 gene promoter were investigated in transgenic Lofus corniculatus plants and shown to constitute a two-component nodule-specific enhancer. Equal quantitative contributions from the two components were suggested by the similar expression level of chimeric N23-chloramphenicol acetyltransferase genes after deletion of either the dista1 positive element (PE-A, -320 to -298) or the proximal positive element (PE-B, -257 to -165). A combined effect of the two elements was indicated by orientationdependent effects in the N23 promoter, and by the observation that neither PE-A nor PE-B separately was able to confer any activity to the cauliflower mosaic virus 35s minimal promoter. Reactivation of the minimal N23 and the minimal cauliflower mosaic virus 35s promoters by the inverted complete element (PE-AB) further suggested that PE-AB is a nodule-specific enhancer containing two equally strong enhancer components. Two 12-bp sequence motifs, lnvA and InvB, constituting an inverted repeat, were identified as the core of the enhancer components PE-A and PE-B, respectively. Point mutations in lnvA or lnvB resulted in lower expression levels and mutations in both abolished enhancer activity. Point mutations in two nodulin consensus sequences, 5'-CTCTT and 5'-AAAGAT located downstream of PE-AB, resulted in a decreased level of expression, confirming the involvement of these two motifs in nodulin gene expression. The binding site for the nodule-specific frans-acting factor, NAT2, present in the PE-A segment, was removed without affecting expression significantly. This interaction is, therefore, dispensable for enhancer activity.

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“…The promoter region of the soybean nodulin N23 gene (Wong and Verma, 1985;Sandal, Bojsen, and Marcker, 1987) was analyzed for binding to leaf and nodule nuclear proteins. Five restriction fragments spanning approximately 1000 bp of the proximal promoter region were incubated with nodule and leaf nuclear extracts.…”

Section: An N23 Promoter Fragment Forms Two Complexes With Nodule Nucmentioning

confidence: 99%

HMG I-like proteins from leaf and nodule nuclei interact with different AT motifs in soybean nodulin promoters.

et al. 1990

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Three different nuclear factors recognizing short AT-rich DNA sequences were identified in different organs of soybean. One factor (NAT2) was found to be present in mature nodules, another factor (NAT1) was detected in roots and nodules, and a third one (LAT1) was only observed in leaves. All three factors recognized several DNA sequences in the promoter region of the soybean nodulin N23 gene. Footprinting, deletion, and point mutation analyses revealed different binding properties for all three factors and further showed that even single base pair substitutions had a dramatic effect on binding affinity. The LATl and NAT1 factors were released from chromatin by extraction with a low-salt buffer and were soluble in 2% trichloroacetic acid, implying a relationship to highmobility group (HMG) proteins. DNA binding studies futther indicated a functional relationship of these factors to the human HMG I protein. Purification of the LATl factor from leaf nuclei revealed the presence of two polypeptides with molecular masses of 21 kilodaltons and 23 kilodaltons, respectively, binding the same DNA sequence with equal affinity.

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Promoter analysis of a soybean nuclear gene coding for nodulin-23, a nodule-specific polypeptide involved in symbiosis with Rhizobium

Cited by 12 publications

References 36 publications

Identification of several soybean cytosolic glutamine synthetase transcripts highly or specifically expressed in nodules: expression studies using one of the corresponding genes in transgenic Lotus corniculatus

Identification of several soybean cytosolic glutamine synthetase transcripts highly or specifically expressed in nodules: expression studies using one of the corresponding genes in transgenic Lotus corniculatus

A two-component nodule-specific enhancer in the soybean N23 gene promoter.

HMG I-like proteins from leaf and nodule nuclei interact with different AT motifs in soybean nodulin promoters.

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