Promoter Analysis in Banana

Santos-Ordóñez, Efrén; Pacheco, Ricardo; Villao, L.; Galarza, Luis; Ochoa, Daniel; Jordán, Carlos; Flores, José

doi:10.1007/978-981-10-1585-4_11

Cited by 7 publications

(3 citation statements)

References 107 publications

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“…Whole-genome sequencing of many plants has facilitated the isolation of promoters from genomes, in which primers can be designed in the 5′ region of the open reading frame [ 12 ]. Once the promoter is isolated and sequenced, in silico analysis can be performed using bioinformatics tools such as PlantCARE [ 13 ], PlantProm DB [ 14 ], iProm-Zea [ 15 ], and TSSPlant [ 16 ]. Recent studies have shown that promoters play an essential role in processes such as gene editing with clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9), increasing the effectiveness and specificity in cutting double-stranded DNA.…”

Section: Introductionmentioning

confidence: 99%

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Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation

Villao-Uzho,

Chávez-Navarrete,

Pacheco-Coello

et al. 2023

Genes

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One of the strategies to overcome diseases or abiotic stress in crops is the use of improved varieties. Genetic improvement could be accomplished through different methods, including conventional breeding, induced mutation, genetic transformation, or gene editing. The gene function and regulated expression through promoters are necessary for transgenic crops to improve specific traits. The variety of promoter sequences has increased in the generation of genetically modified crops because they could lead to the expression of the gene responsible for the improved trait in a specific manner. Therefore, the characterization of the promoter activity is necessary for the generation of biotechnological crops. That is why several analyses have focused on identifying and isolating promoters using techniques such as reverse transcriptase-polymerase chain reaction (RT-PCR), genetic libraries, cloning, and sequencing. Promoter analysis involves the plant genetic transformation method, a potent tool for determining the promoter activity and function of genes in plants, contributing to understanding gene regulation and plant development. Furthermore, the study of promoters that play a fundamental role in gene regulation is highly relevant. The study of regulation and development in transgenic organisms has made it possible to understand the benefits of directing gene expression in a temporal, spatial, and even controlled manner, confirming the great diversity of promoters discovered and developed. Therefore, promoters are a crucial tool in biotechnological processes to ensure the correct expression of a gene. This review highlights various types of promoters and their functionality in the generation of genetically modified crops.

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Section: Introductionmentioning

confidence: 99%

“…Promoters can be classified as constitutive, tissue-specific, inducible, and synthetic. The classification will depend on the function, type, and gene expression level [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation

Villao-Uzho,

Chávez-Navarrete,

Pacheco-Coello

et al. 2023

Genes

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“…Usually, six to twelve months could last to obtain transgenic in vitro banana plants after Agrobacterium-mediated transformation of embryogenic cell suspensions. Furthermore, the development of embryogenic cell suspensions could take eight to twelve months 2,3,5,6,7 . Therefore, the genetic transformation from in vitro banana plants was performed through Agrobacterium tumefaciens; and confirmation of transformation was performed by using the luciferase reporter gene system and PCR.…”

Section: Introductionmentioning

confidence: 99%

Genetic transformation of apical meristematic shoots in the banana cultivar ‘Williams’

Villao

Flores

Santos-Ordóñez

2021

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Bananas and plantains (Musa spp.) are among the most critical socioeconomic crops globally, being a staple food for millions of people in the tropics and an essential component for the export market, including the subtropics. Besides conventional breeding, genetic improvement of bananas and plantains could be performed through genetic engineering and new breeding techniques. Furthermore, plant tissue culture is essential for these technologies, including developing embryogenic cell suspensions and in vitro plants. The transient and stable genetic transformation could be performed from in vitro plants, shortening Musa transgenic lines development compared to genetic transformation while using embryogenic cell suspension. In this study, a genetic transformation protocol was established from banana apical meristems for the ‘Williams’ cultivar (genotype AAA). The protocol was based on the co-cultivation of the explants (whole in vitro plants or bisected meristematic tissues derived from in vitro plants) with Agrobacterium tumefaciens strain LBA4404 harboring two binary vectors denominated pLVCIBE1 (cassette: MabHIPP promoter::luc2::Tnos, P35S::hpt::Tnos) and pLVCIBE2 (cassette: P35S::luc2::Tnos, P35S::hpt::Tnos), independently. The stable genetic transformation was obtained by subculturing in vitro banana plants in selection medium (12.5µg/mL of hygromycin) for 8 weeks from bisected meristematic tissue transformation. Genetic transformation was confirmed in vivo with the use of the luciferase reporter gene system. Furthermore, PCR was performed on DNA extracted from leaves of regenerated transgenic in vitro plants after 8 weeks of selection, confirming stable genetic transformation. Therefore, genetic transformation was achieved in the apical meristematic tissue of in vitro banana plants with co-cultivation of Agrobacterium tumefaciens.

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Complementation of Bananas Conventional Breeding Programs Through Biotechnological Genetic Improvement

López

Santos-Ordóñez

González

2020

Agricultural, Forestry and Bioindustry Biotechnology and Biodiscovery

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Promoter Analysis in Banana

Cited by 7 publications

References 107 publications

Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation

Plant Promoters: Their Identification, Characterization, and Role in Gene Regulation

Genetic transformation of apical meristematic shoots in the banana cultivar ‘Williams’

Complementation of Bananas Conventional Breeding Programs Through Biotechnological Genetic Improvement

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