Promising epiphytic antagonistic Pseudomonas strains from the Citrus phyllosphere in the biocontrol of Pseudomonas syringae pv. syringae, causing bacterial citrus blast and black pit

Abdellatif, E.; Mikiciński, Artur; Kałużna, Monika; Bahri, Bochra Amina; Mougou‐Hamdane, Amira; Vaerenberg, Johan van; Baeyen, Steve; Rhouma, Ali; Janse, Jacob Dirk

doi:10.1007/s42161-022-01133-y

Cited by 2 publications

(1 citation statement)

References 61 publications

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“…The phyllosphere, or the above ground parts of plants, is known to house diverse interacting organisms, including microbes and herbivorous insects (Humphrey et al 2014; Bashir et al 2022). Bacteria in the genus Pseudomonas (pseudomonads) are common members of phyllosphere communities and are often studied for their direct impacts on plants as pathogens or beneficial taxa (Passera et al 2019; Abdellatif et al 2022; Agbavor et al 2022). However, interactions of pseudomonads with herbivorous insects can also have important ecological outcomes (Humphrey et al 2014; Smee and Hendry 2022).…”

Section: Introductionmentioning

confidence: 99%

Common fluorescent Pseudomonas in the phyllosphere can influence aphid behavior in diverse ways

Herr,

Schieber,

Hendry

2024

Preprint

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Bacteria in the phyllosphere, the above ground parts of plants, can have complex interactions with plants and their insect visitors. For instance, certain Pseudomonas strains change pea aphid (Acyrthosiphon pisum) feeding behavior. Aphids visually detect and avoid feeding when Pseudomonas produces a compound with blue fluorescent emissions, the siderophore pyoverdine. It is unknown how commonly such interactions occur in nature, so we investigated this potential in pea plant (Pisum sativum) phyllosphere communities. We found a diversity of Pseudomonas taxa with fluorescent potential in pea plant microbiomes. Culture isolates revealed a wide range of fluorescent emissions spectra across taxa, which produced blue to green fluorescence of varying intensities. We tested strains from across this emissions range for pea aphid behavioral responses when given a choice between plants inoculated with a fluorescent isolate or a control treatment. Consistent with previous work, we found that some isolates were avoided by aphids. Surprisingly, some isolates were actually attractive to aphids, causing up to 70% of aphids to settle and feed on bacterially treated plants. Attractive isolates produced green fluorescence in culture, suggesting that attraction could be due to aphid sensory biases. Overall, we found both isolate fluorescence in culture and siderophore potential inferred from gene content to be poor predictors of aphid behavior. We also found very high variability in responses across replicate experiments for some strains, suggesting that environmental conditions may influence outcomes. This shows that bacterial fluorescence may be common on plants and can have context-dependent impacts on herbivorous insects.

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Section: Introductionmentioning

confidence: 99%

Common fluorescent Pseudomonas in the phyllosphere can influence aphid behavior in diverse ways

Herr,

Schieber,

Hendry

2024

Preprint

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Bacteria intrinsic to Medicago sativa (alfalfa) reduce Salmonella enterica growth in planta

Vitt,

Hansen,

Garg

et al. 2023

Journal of Applied Microbiology

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Aims The purpose of this study was to determine whether plant-associated bacteria (PAB) can reduce Salmonella enterica colonization and infection of alfalfa sprouts to reduce the risk of foodborne illness. Methods We isolated plant associated bacteria from alfalfa seeds and sprouts. Monoclonal isolates of the bacteria were obtained and tested for their ability to inhibit Salmonella Typhimurium growth in alfalfa sprouts over 6-days. Genome sequencing and annotation was used to construct draft genomes of the bacteria isolated in this study using Illumina sequencing platform. Results We observed that a cocktail of five PAB could reduce Salmonella growth in alfalfa sprouts from ∼108 CFU g−1 to ∼105 CFU g−1, demonstrating a protective role. Genome sequencing revealed that these bacteria were members of the Pseudomonas, Pantoea, and Priestia genus, and did not possess genes that were pathogenic to plants or animals. Conclusions This work demonstrates that PAB can be utilized to reduce pathogen levels in fresh produce which may be synergistic with other technologies to improve the safety of sprouts and other fresh produce.

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Promising epiphytic antagonistic Pseudomonas strains from the Citrus phyllosphere in the biocontrol of Pseudomonas syringae pv. syringae, causing bacterial citrus blast and black pit

Cited by 2 publications

References 61 publications

Common fluorescent Pseudomonas in the phyllosphere can influence aphid behavior in diverse ways

Common fluorescent Pseudomonas in the phyllosphere can influence aphid behavior in diverse ways

Bacteria intrinsic to Medicago sativa (alfalfa) reduce Salmonella enterica growth in planta

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