Promising approaches towards biotransformation of polycyclic aromatic hydrocarbons with Ascomycota fungi

Aranda, Elisabet

doi:10.1016/j.copbio.2015.12.002

Cited by 114 publications

(50 citation statements)

References 61 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In this study, we showed the ability of some aquatic Ascomycetes to degrade large polymers. This is consistent with previous works showing the capacity of members of the phylum Ascomycota to degrade different types of polymeric substances including, i.e., aromatic hydrocarbons and synthetic azo and anthraquinones (Junghanns et al ; Aranda ; Wang et al ). Additionally, considering that a large proportion of aquatic fungi belongs to this phylum, while Basidiomycota are less represented in this environment (Shearer et al ; Bärlocher and Boddy ), it is likely that members of Ascomycota might dominate fungal processing of HS in aquatic systems.…”

Section: Resultssupporting

confidence: 93%

Transformation of humic substances by the freshwater Ascomycete Cladosporium sp.

Rojas-Jiménez

Fonvielle

et al. 2017

Limnology & Oceanography

View full text Add to dashboard Cite

The ecological relevance of fungi in freshwater ecosystems is becoming increasingly evident, particularly in processing the extensive amounts of polymeric organic carbon such as cellulose, chitin, and humic substances (HS). We isolated several fungal strains from oligo-mesotrophic Lake Stechlin, Brandenburg, Germany, and analyzed their ability to degrade polymeric-like substrates. Using liquid chromatography-organic carbon detection, we determined the byproducts of HS transformation by the freshwater fungus Cladosporium sp. KR14. We demonstrate the ability of this fungus to degrade and simultaneously synthesize HS, and that transformation processes were intensified when iron, as indicator of the occurrence of Fenton reactions, was present in the medium. Furthermore, we showed that structural complexity of the HS produced changed with the availability of other polymeric substances in the medium. Our study highlights the contribution of freshwater Ascomycetes to the transformation of complex organic compounds. As such, it has important implications for understanding the ecological contribution of fungi to aquatic food webs and related biogeochemical cycles.

show abstract

Section: Resultssupporting

confidence: 93%

Transformation of humic substances by the freshwater Ascomycete Cladosporium sp.

Rojas-Jiménez

Fonvielle

et al. 2017

Limnology & Oceanography

View full text Add to dashboard Cite

show abstract

“…Cytochrome P450 (CYP) enzymes comprise another group of enzymes of which their involvement in the degradation of some organic compounds by WRF has been demonstrated (Ning and Wang 2012; Kelly and Kelly 2013; Zhang et al 2015). CYPs are membrane-bound hemoproteins that catalyze hydroxylation, epoxidation and monooxygenation reactions (Neve and Ingelman-Sundberg 2008; Aranda 2016). In fungi, CYPs are involved in the biosynthesis of secondary metabolites and ergosterol, and also in catabolic reactions that lead to the degradation of xenobiotic compounds (Aranda 2016).…”

Section: Introductionmentioning

confidence: 99%

“…CYPs are membrane-bound hemoproteins that catalyze hydroxylation, epoxidation and monooxygenation reactions (Neve and Ingelman-Sundberg 2008; Aranda 2016). In fungi, CYPs are involved in the biosynthesis of secondary metabolites and ergosterol, and also in catabolic reactions that lead to the degradation of xenobiotic compounds (Aranda 2016). …”

Section: Introductionmentioning

confidence: 99%

Bioremoval of humic acid from water by white rot fungi: exploring the removal mechanisms

et al. 2016

View full text Add to dashboard Cite

Twelve white rot fungi (WRF) strains were screened on agar plates for their ability to bleach humic acid (HA). Four fungal strains were selected and tested in liquid media for removal of HA. Bioremediation was investigated by HA color removal and changes in the concentration and molecular size distribution of HA by size exclusion chromatography. Trametes versicolor and Phanerochaete chrysosporium showed the highest HA removal efficiency, reaching about 80%. Laccase and manganese peroxidase were measured as extracellular enzymes and their relation to the HA removal by WRF was investigated. Results indicated that nitrogen limitation could enhance the WRF extracellular enzyme activity, but did not necessarily increase the HA removal by WRF. The mechanism of bioremediation by WRF was shown to involve biosorption of HA by fungal biomass and degradation of HA to smaller molecules. Also, contradicting previous reports, it was shown that the decolorization of HA by WRF could not necessarily be interpreted as degradation of HA. Biosorption experiments revealed that HA removal by fungal biomass is dependent not only on the amount of biomass as the sorbent, but also on the fungal species. The involvement of cytochrome P450 (CYP) enzymes was confirmed by comparing the HA removal capability of fungi with and without the presence of a CYP inhibitor. The ability of purified laccase from WRF to solely degrade HA was proven and the importance of mediators was also demonstrated.

show abstract

“…This suggests an activation of the detoxification pathway, probably promoted by the phenolics present in the ADOR as well as by the metabolites and radicals formed during the LME transformation. By-products from the primary extracellular attack could enter through the mycelia, continuing the transformation in the cytoplasm, as occurs in transformations of aromatic compounds (Aranda, 2016). Thus, this data could indicate the involvement of intracellular mechanisms (direct and indirect) in the detoxification of the residue within the cells, which was for the first time studied in the present work using agro-wastes.…”

Section: Intracellular Enzymatic Activity In C Purpureummentioning

confidence: 84%

Enzymatic mechanisms and detoxification of dry olive-mill residue by Cyclocybe aegerita, Mycetinis alliaceus and Chondrostereum purpureum

Reina

Liers

García-Romera

et al. 2017

International Biodeterioration & Biodegradation

Self Cite

View full text Add to dashboard Cite

Promising approaches towards biotransformation of polycyclic aromatic hydrocarbons with Ascomycota fungi

Cited by 114 publications

References 61 publications

Transformation of humic substances by the freshwater Ascomycete Cladosporium sp.

Transformation of humic substances by the freshwater Ascomycete Cladosporium sp.

Bioremoval of humic acid from water by white rot fungi: exploring the removal mechanisms

Enzymatic mechanisms and detoxification of dry olive-mill residue by Cyclocybe aegerita, Mycetinis alliaceus and Chondrostereum purpureum

Contact Info

Product

Resources

About