Promising Applications of Tumor Spheroids and Organoids for Personalized Medicine

Gilazieva, Zarema; Ponomarev, Aleksei S.; Rutland, Catrin S.; Rizvanov, Albert A.; Solovyeva, Valeriya V.

doi:10.3390/cancers12102727

Cited by 94 publications

(85 citation statements)

References 143 publications

(190 reference statements)

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“…[35,37] The epithelialmesenchymal interactions have been shown to guarantee the stability of the 3D culture at long-term (at least 1 year) [35] mostly due to in vitro expansion of immature progenitor populations, replenishing dying cells; this type of interaction is one of the characteristics presented by organoids, while not all spheroids are viable for longer periods in culture for not necessarily presenting regular morphology and also due to spheroid transferring, what can cause disaggregation. [38,39] of cells to self-organize spontaneously, deposit extracellular matrix and form specific microenvironments. [26,[40][41][42] In oncology research, several trials have shown that while monolayer tumors were sensitive to the action of several chemotherapeutic agents, the same cells, when grown as spheroids, were resistant to them.…”

Section: Particularities Of the Most Common 3d Cultures: Spheroids And Organoidsmentioning

confidence: 99%

Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine

Sakalem

Síbio

Costa

et al. 2021

Biotechnology Journal

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Background: An impressive percentage of biomedical advances were achieved through animal research and cell culture investigations. For drug testing and disease researches, both animal models and preclinical trials with cell cultures are extremely important, but present some limitations, such as ethical concern and inability of representing complex tissues and organs. 3D cell cultures arise providing a more realistic in vitro representation of tissues and organs. Environment and cell type in 3D cultures can represent in vivo conditions and thus provide accurate data on cell-to-cell interactions, and cultivation techniques are based on a scaffold, usually hydrogel or another polymeric material, or without scaffold, such as suspended microplates, magnetic levitation, and microplates for spheroids with ultra-low fixation coating. Purpose and scope:This review aims at presenting an updated summary of the most common 3D cell culture models available, as well as a historical background of their establishment and possible applications. Summary:Even though 3D culturing is incapable of replacing other current research types, they will continue to substitute some unnecessary animal experimentation, as well as complement monolayer cultures. Conclusion:In this aspect, 3D culture emerges as a valuable alternative to the investigation of functional, biochemical, and molecular aspects of human pathologies.

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Section: Particularities Of the Most Common 3d Cultures: Spheroids And Organoidsmentioning

confidence: 99%

Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine

Sakalem

Síbio

Costa

et al. 2021

Biotechnology Journal

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“…Each model (spheroids vs. organoids) has its own advantages and disadvantages as reviewed by Gilazieva et al [ 64 ]. For example, spheroid models are thought to be enriched for a cell population with a CSC phenotype; however, these models may not fully recapitulate the tumor architecture [ 64 ]. On the other hand, organoid models are more architecturally and functionally similar to their parental tumors and can be maintained in culture long term through passaging [ 64 ].…”

Section: Emergence Of 3d Cell Culture Models For Drug-testing In Omentioning

confidence: 99%

“…For example, spheroid models are thought to be enriched for a cell population with a CSC phenotype; however, these models may not fully recapitulate the tumor architecture [ 64 ]. On the other hand, organoid models are more architecturally and functionally similar to their parental tumors and can be maintained in culture long term through passaging [ 64 ]. For use in drug testing assays, particular consideration should be given to the limitations associated with each model.…”

Section: Emergence Of 3d Cell Culture Models For Drug-testing In Omentioning

confidence: 99%

“…For use in drug testing assays, particular consideration should be given to the limitations associated with each model. For example, due to their compact 3D structure tumor spheroids may create a drug penetration gradient, while drug responses of organoids may be impacted by the ECM in which cells are embedded [ 64 ].…”

Section: Emergence Of 3d Cell Culture Models For Drug-testing In Omentioning

confidence: 99%

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Exploring the Potential of Drug Response Assays for Precision Medicine in Ovarian Cancer

Singh

Neal

Moatamed

et al. 2020

IJMS

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One of the major challenges in the treatment of cancer are differential responses of patients to existing standard of care anti-cancer drugs. These differential responses may, in part, be due to a diverse range of genomic, epigenomic, proteomic, and metabolic alterations among individuals suffering from the same type of cancer. Precision medicine is an emerging approach in cancer therapeutics that takes into account specific molecular alterations, environmental factors as well as lifestyle of individual patients. This approach allows clinicians and researchers to select or predict treatments that would most likely benefit the patient based on their individual tumor characteristics. One class of precision medicine tools are predictive, in vitro drug-response assays designed to test the sensitivity of patient tumor cells to existing or novel therapies. These assays have the potential to rapidly identify the most effective treatments for cancer patients and thus hold great promise in the field of precision medicine. In this review, we have highlighted several drug-response assays developed in ovarian cancer and discussed the current challenges and future prospects of these assays in the clinical management of this disease.

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“…Applications of spheroids from different cellular sources have been reviewed extensively elsewhere and hence we mainly focus on tumor-derived organoids. However, we include recent literature on tumor-derived spheroids in respective sections [ 10 , 11 , 12 ]. Finally, we conclude with merits and challenges of organoid technology in cancer biology.…”

Section: Introductionmentioning

confidence: 99%

Organoid and Spheroid Tumor Models: Techniques and Applications

Gunti

Hoke

et al. 2021

Cancers

248

205

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Techniques to develop three-dimensional cell culture models are rapidly expanding to bridge the gap between conventional cell culture and animal models. Organoid and spheroid cultures have distinct and overlapping purposes and differ in cellular sources and protocol for establishment. Spheroids are of lower complexity structurally but are simple and popular models for drug screening. Organoids histologically and genetically resemble the original tumor from which they were derived. Ease of generation, ability for long-term culture and cryopreservation make organoids suitable for a wide range of applications. Organoids-on-chip models combine organoid methods with powerful designing and fabrication of micro-chip technology. Organoid-chip models can emulate the dynamic microenvironment of tumor pathophysiology as well as tissue–tissue interactions. In this review, we outline different tumor spheroid and organoid models and techniques to establish them. We also discuss the recent advances and applications of tumor organoids with an emphasis on tumor modeling, drug screening, personalized medicine and immunotherapy.

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Promising Applications of Tumor Spheroids and Organoids for Personalized Medicine

Cited by 94 publications

References 143 publications

Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine

Historical evolution of spheroids and organoids, and possibilities of use in life sciences and medicine

Exploring the Potential of Drug Response Assays for Precision Medicine in Ovarian Cancer

Organoid and Spheroid Tumor Models: Techniques and Applications

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