Promiscuous nodulation of Phaseolus vulgaris, Macroptilium atropurpureum, and Leucaena leucocephala by indigenous Rhizobium meliloti

Bromfield, E. S. P.; Barran, L. R.

doi:10.1139/m90-065

Cited by 46 publications

(37 citation statements)

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“…nov. strains. Under laboratory conditions, beans nodulate with a wide range of rhizobia (6,18,24, 34, 39, 52), in many cases effectively (39). A comprehensive taxoqomy of these strains will require further research.…”

Section: Discussionmentioning

confidence: 99%

Rhizobium tropici, a Novel Species Nodulating Phaseolus vulgaris L. Beans and Leucaena sp. Trees

Martı́nez-Romero

Segovia

Mercante

et al. 1991

International Journal of Systematic Bacteriology

584

340

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A new Rhizobium species that nodulates PhaseoZus vulgaris L. and Leucaena spp. is proposed on the basis of the results of multilocus enzyme electrophoresis, DNA-DNA hybridization, an analysis of ribosomal DNA organization, a sequence analysis of 16s rDNA, and an analysis of phenotypic characteristics. This taxon, Rhizobium tropici sp. nov., was previously named Rhizobium Zeguminosarum biovar phaseoli (type I1 strains) and was recognized by its host range (which includes Leucaena spp.) and nifgene organization. In contrast to R. Zeguminosarum biovar phaseoli, R. tropici strains tolerate high temperatures and high levels of acidity in culture and are symbiotically more stable. We identified two subgroups within R . tropici and describe them in this paper.Members of the genus Rhizobium nodulate the roots of leguminous plants. The rhizobia that infect peas, clovers, and beans (Phaseolus vulgaris L.) are clustered in a single species, Rhizobium leguminosarum (29), which has three biovars (Rhizobium leguminosarum biovar viciae, Rhizobium leguminosarum biovar trifolii, and Rhizobium leguminosarum biovar phaseoli); these biovars contain different symbiotic plasmids that encode distinct nodulation specificities. Nevertheless, heterogeneity in Rhizobium leguminosarum biovar phaseoli has been identified by using such different criteria as protein pattern (50), antibiotic resistance (2), serological type (49), multilocus enzyme electrophoresis behavior (45), DNA-DNA hybridization data (10, 26, 54), plasmid profile (37) , and exopolysaccharide structure (70).We previously distinguished two different types among isolates obtained from bean nodules and found differences in their symbiotic plasmids (36, 38, 39). Type I strains have multiple copies of nitrogenase n i m genes (39, 46), a narrow nodulation host range, and hybridize with the psi (polysaccharide inhibition) gene (3). Type I1 strains have single copies of nifgenes, nodulate Leucaena spp., and do not hybridize with the psi gene (36, 39).Type I1 strains have received attention because their symbiotic plasmids promote an effective and completely differentiated symbiotic process in Agrobacterium tumefaciens recipients (5, 38). They are genetically stable, retaining their symbiotic plasmid after prolonged incubation at 37°C. Some are heat tolerant (31) or acid and aluminum resistant (12, 25, 30, 62). The nodulation genes from one of these strains have been cloned (64). The chemical composition and structure of the extracellular polysaccharides from one type I1 strain differ from the chemical composition and structure of the extracellular polysaccharides from type I isolates (23).Type I1 strains have been less successful in competition for bean nodule occupancy than the type I strains used (41). The former have been reported to occur less frequently in * Corresponding author bean nodules (39). Nodule occupancy by type I1 strains can be enhanced under acid conditions (47, 63).To define the taxonomic position and the genetic relatedness of type I1 strains, we analyzed 64 typ...

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Section: Discussionmentioning

confidence: 99%

Rhizobium tropici, a Novel Species Nodulating Phaseolus vulgaris L. Beans and Leucaena sp. Trees

Martı́nez-Romero

Segovia

Mercante

et al. 1991

International Journal of Systematic Bacteriology

584

340

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“…Some of the isolates that were similar to R. tropici IIB by RFLP-PCR of the 16S rRNA gene had sequences similar to those of Agrobacterium; however, R. tropici IIB and A. rhizogenes cannot be differentiated by restriction site analysis of the 16S rRNA gene (19,21). P. vulgaris is known to nodulate with S. meliloti strains (7,16).…”

Section: S Rdna Pcr-rflpmentioning

confidence: 99%

The Diversity of Phaseolus -Nodulating Rhizobial Populations Is Altered by Liming of Acid Soils Planted with Phaseolus vulgaris L. in Brazil

Andrade

Murphy

Giller

2002

Appl Environ Microbiol

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PCR-mediated restriction fragment length polymorphism (RFLP) analysis of the 16S-23S rRNA internally transcribed spacer (ITS) region and the 16S rRNA gene indicated that the rhizobial populations isolated from common bean (Phaseolus vulgaris L.) nodules in the unlimed soil from a series of five lime rates applied 6 years previously to plots of an acidic oxisol had less diversity than those from plots with higher rates of liming. Isolates affiliated with Rhizobium tropici IIB and Rhizobium leguminosarum bv. phaseoli were predominant independent of lime application. An index of richness based on the number of ITS groups increased from 2.2 to 5.7 along the soil liming gradient, and the richness index based on "species" types determined by RFLP analysis of the 16S rRNA gene varied from 0.5 to 1.4. The Shannon index of diversity, based on the number of ITS groups, increased from 1.8 in unlimed soil to 2.8 in limed soil, and, based on RFLP analysis of the 16S rRNA gene, ranged from 0.9 to 1.4. In the limed soil, the subpopulation of R. tropici IIB pattern types contained the largest number of ITS groups. In contrast, there were more R. leguminosarum bv. phaseoli types in the unlimed soil with the lowest pH than in soils with the highest pH. The number of ITS ("strain") groups within R. leguminosarum bv. phaseoli did not change with increased abundance of rhizobia in the soil, while with R. tropici IIB, the number of strain groups increased significantly. Some cultural and biochemical characteristics of Phaseolus-nodulating isolates were significantly related to changes in soil properties caused by liming, largely due to changes in the predominance of the rhizobial species groups.

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“…Esto no es extraño, debido a que la asociación simbiótica establecida entre esta especie vegetal y B. japonicum además de otros géneros rizobianos que incluyen R. etli, R. tropici, S. fredii, M. loti, R. leguminosarum bvs. trifolii y viciae, S. meliloti y Rhizobium spp., ya han sido señaladas por Bromfield & Barran (1990) y Michiels et al(1998) como un hospedador no selectivo, aunque la mayoría de estas asociaciones no eran efectivas. Sin embargo, el aislado Nod 2 R resultó ser un microsimbionte eficiente en cuanto a la capacidad fijadora de nitrógeno (datos no publicados).…”

Section: Discussionunclassified

“…La autenticación de los aislados se realizó en Macroptilium lathyroides (Siratro) (Bromfield & Barran 1990) y Crotalaria sp. Previamente se escarificaron las semillas con papel lija Nº 280, y se esterilizó con hipoclorito 0.5% durante 7min; se germinaron a 26ºC en medio TY agarizado (Beringer 1974) y se sembraron en recipientes plásticos (7 x 15cm) con arena estéril, impregnada inicialmente con solución Hoagland (Taiz & Zeiger 1998) con el nitrógeno diluido a la mitad (nitrógeno de arranque).…”

Section: Prueba De Infectividadunclassified

Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela

Marquina¹,

González²,

Castro³

2010

RBT

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Phenotypic and genotypic characterization of twelve rhizobial isolates from different regions of Venezuela. Rhizobial taxonomy and systematics have progressed substantially, nevertheless, few studies have been developed on venezuelan species. This study evaluated the phenotypic and genetic variation between 12 venezuelan indigenous rhizobial isolates and 10 international referential strains, by phenotypical traits and DNA molecular markers. in this regard, a PCR-RFLP of the 16S rDNA gene, the presence of large plasmids, metabolic assays in solid media, salinity resistance, pH and temperature growth conditions, and intrinsic antibiotic resistance were assayed. in reference to the phenotypic attributes, we recognized three main groups: A group i, which comprised all the strains metabolizing between 67.5%-90% of the C and N sources. They were also acid-tolerant, as well as acid producers, capable of growing at 40ºC and in high salinity conditions (2-2.5% NaCl). With regard to the antibiotic sensitivity, this group was susceptible to a 30% of the antibiotic assayed. Strains belonging to Group ii exhibited a lower salt tolerance (0.1-1.5%NaCl), as well as a lower acid tolerance, since they grew well at pH values equal or higher than 5.0. This group appeared to be resistant to all of the antibiotics assayed and only metabolized between 52.5%-82.5% of the C and N sources. Group iii was represented by a single bacterial strain: it has a extremely low salt tolerance (0.1% NaCl). This strain grew at a pH equal or higher than 5.6, was susceptible to 50% of the antibiotics assayed and metabolized 72% of the C and N sources. On the basis of a PCR-RFLP of the 16S rDNA, three groups were also obtained. Members of the group A showed a close resemblance to Rhizobium tropici CiAT 899 and Sinorhizobium americanum CFN-Ei 156, while Group B was closely related to Bradyrhizobium spp. Group C, was also represented by only one isolate. The Trebol isolate, was the only one strain able to form nodules and does not appear to be related to any of the referential rhizobial strains, suggesting a possible symbiotic horizontal gene transfer. Finally, in this work, there are evidences of a genetic diversity in the venezuelan rhizobial strains. A different geographical origin is perhaps an important factor affecting the diversity of the indigenous rhizobia in this study. Rev. Biol. Trop. 59 (3): 1017-1036. Epub 2011 September 01.Key words: Rhizobium, Bradyrhizobium, phenotypic characterization, PCR-RFLP, 16S rDNA.Las bacterias denominadas comúnmen-te rizobios presentan varias formas de vida; pueden comportarse como saprófitos en el suelo establecer una asociación simbiótica y formar nódulos con las raíces y tallos de las leguminosas, donde tiene lugar la reducción del nitrógeno atmosférico en amonio, el cual es transportado a la planta y convertido en biomoléculas esenciales, o bien estar presentes como endófito en raíces de diferentes especies vegetales, donde ejercen efectos promotores del crecimiento (Wang et al. 2001).La taxonomía ...

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Promiscuous nodulation of Phaseolus vulgaris, Macroptilium atropurpureum, and Leucaena leucocephala by indigenous Rhizobium meliloti

Cited by 46 publications

References 0 publications

Rhizobium tropici, a Novel Species Nodulating Phaseolus vulgaris L. Beans and Leucaena sp. Trees

Rhizobium tropici, a Novel Species Nodulating Phaseolus vulgaris L. Beans and Leucaena sp. Trees

The Diversity of Phaseolus -Nodulating Rhizobial Populations Is Altered by Liming of Acid Soils Planted with Phaseolus vulgaris L. in Brazil

Caracterización fenotípica y genotípica de doce rizobios aislados de diversas regiones geográficas de Venezuela

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