Promicromonospora flava sp. nov., isolated from sediment of the Baltic Sea

Abstract

A Gram-positive, non-spore-forming actinomycete, designated strain CC 0387 T , was isolated from a sediment sample from the Baltic Sea, Germany. Classification using a polyphasic approach and comparative 16S rRNA gene sequencing showed that strain CC 0387 T belonged to the genus Promicromonospora and displayed more than 3 % 16S rRNA gene sequence divergence from all Promicromonospora species with validly published names. Strain CC 0387 T did not produce aerial mycelium. Substrate mycelia were yellowish white to pale orange-yellow and fragmented into bacillary or coccoid elements. The cell wall contained lysine and alanine. Wholecell hydrolysates contained galactose, glucose, rhamnose and ribose. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, an unknown phospholipid and an unknown glycolipid. The predominant menaquinone was MK-9(H 4 ) (86 %). The G+C content of the DNA was 71.87 mol%. Based on morphological, chemotaxonomic, phenotypic and genetic characteristics, strain CC 0387 T (5CCTCC AA208024 T 5DSM 21481 T

Distribution and abundance of microflora in sandy beaches on the southern coast of the Baltic Sea

“…Actinomycetes are gram positive, non-spore forming bacteria with high G+C content in their DNA (Deepika & Kannabiran 2010, Ogunmwonyi et al 2010. The presence of actinomycetes, mainly of the genera Streptomyces, Nocardia and Nocardiform, has been detected in sediment and seawater in marine ecosystems (Sharma & Pant 2001, Remya & Vijayakumar 2008, Jiang et al 2009). Actinomycetes play a major role in the recycling of organic matter and contribute significantly to the turnover of complex biopolymers, such as lignocellulose, hemicellulose, lignin, pectin, chitin, keratin, crude oil and hydrocarbons (Suzuki et al 1994, Sharma & Pant 2001, Zaitlin et al 2004, Vijayakumur et al 2007, Reyma & Vijayakumur 2008.…”

Promicromonospora umidemergens sp. nov., isolated from moisture from indoor wall material

“…Chemotaxonomic characteristics that differentiate the type strains of members of the genus Promicromonospora Strains: 1, 09-Be-007 T ; 2, P. vindobonensis V45 T ; 3, P. aerolata V54A T ; 4, P. kroppenstedtii RS16 T ; 5, P. citrea DSM 43110 T ; 6, P. sukumoe DSM 44121 T ; 7, P. flava CC 0387 T . Data are from this study, Takahashi et al (1987), Kalakoutskii et al (1989), Busse et al (2003) and Jiang et al (2009 Strains: 1, 09-Be-007 T ; 2, P. vindobonensis V45 T ; 3, P. aerolata V54A T ; 4, P. kroppenstedtii RS16 T ; 5, P. sukumoe DSM 44121 T ; 6, P. citrea DSM 43110 T ; 7, P. flava CC 0387 T . All strains were grown on TSA for 48 h at 28 u C and analysed using the MIDI system (Kämpfer & Kroppenstedt, 1996).…”

“…The genus Promicromonospora currently harbours six species: Promicromonospora citrea (Krasil'nikov et al, 1961), Promicromonospora sukumoe (Takahashi et al, 1987), Promicromonospora aerolata and Promicromonospora vindobonensis (Busse et al, 2003), Promicromonospora kroppenstedtii (Alonso-Vega et al, 2008), and Promicromonospora flava (Jiang et al, 2009). Two species originally described as members of the genus Promicromonospora have been transferred to other genera: Promicromonospora enterophila (Jäger et al, 1983) was transferred to the genus Oerskovia as Oerskovia enterophila (Stackebrandt et al, 2002), and Promicromonospora pachnodae (Cazemier et al, 2003) was reclassified as Xylanimicrobium pachnodae (Stackebrandt & Schumann, 2004).…”

Promicromonospora endophytica sp. nov., an endophytic actinobacterium isolated from the root of an Australian native Grey Box tree

“…At the time of writing, the genus Promicromonospora contained eight species: P. citrea (Krasil'nikov et al, 1961), P. sukumoe (Takahashi et al, 1987), P. aerolata and P. vindobonensis (Busse et al, 2003), P. kroppenstedtii (Alonso-Vega et al, 2008), P. flava (Jiang et al, 2009), P. umidemergens (Martin et al, 2010 and P. xylanilytica (Qin et al, 2012). P. enterophila (Jäger et al, 1983) was transferred to the genus Oerskovia as Oerskovia enterophila (Stackebrandt et al, 2002) and P. pachnodae (Cazemier et al, 2003) was reclassified as Xylanimicrobium pachnodae (Stackebrandt & Schumann, 2004).…”