Prolylcarboxypeptidase (Angiotensinase C): Purification and Characterization of the Enzyme fromXanthomonas maltophilia

Prolyl carboxypeptidase purified from human placenta: its characterization and identification as an apelin-cleaving enzyme

“…This enzyme is sensitive to high temperatures while PRCP is not [19]. PRCP protein purification was mainly based on ion exchange and lectin affinity chromatography [21]. As PRCP has six potential N-glycosylation sites [27], a Concanavalin Asepharose column was chosen to bind the glycosylated enzyme.…”

“…So far, human PRCP has only been purified from kidneys and neutrophils [17,18]. Searching the literature, a few reports on the purification of PRCP from other species were found (overview in the Supplementary Material) [19][20][21][22]. The enzyme is found both intracellularly in lysosomes and extracellularly as a cell membrane-bound (e.g.…”

On the Mechanism of Action of Prolylcarboxypeptidase

“…Wallingford and associates described that Prcp-null mice had elevated levels of alpha-MSH1-13 in the hypothalamus and were leaner and shorter than the wild-type controls on a regular chow diet. Prcp null mice were also resistant to high-fat diet-induced obesity Soisson and associates described the crystal structure of human PRCP E112D polymorphism in the prolylcarboxypeptidase gene was found to be associated with blood pressure response to benazepril in Chinese hypertensive patients Javerzat and associates described overexpression of PRCP during the intermediate phase of the chick chorio-allantoic membrane Recombinant PRCP (rPRCP) metabolized BK1-8 to BK1-7, whereas rPRCP was ineffective in metabolizing BK 1-9 PRCP was found to be active in human cerebrospinal fluid Overexpression of angiotensin type 2 (AT2) receptor in mouse coronary artery endothelial cells was found to increase expression of PRCP, which may contribute to kinin release PRCP is a 4OHTAM resistance factor in estrogen receptor-positive breast cancer cells PRCP gt/gt mice were found to be hypertensive and prothrombotic (Sorrells and Erdos, 1971) (Odya et al, 1978) (Skidgel et al, 1981) (Deddish et al, 1990) (Tan et al, 1993) (Tamaoki et al, 1994) (Suzawa et al, 1995) (Suga et al, 1995) (Jackman et al, 1995) (Watson, Jr. et al, 1997) To deal with proper delivery of blood to the tissues as well as many types of insults, including chemical and physical damage as well as infection, the cardiovascular system has evolved an intricate multilayer of specialized biochemical pathways. Multiple feedback mechanisms controlling blood flow with contrasting effects regulate normal vascular function, which have either stimulatory or inhibitory effects.…”

Prolidase fromXanthomonas maltophilia: Purification and Characterization of the Enzyme

“…23 ) Regarding the X. maltophilia strain, the detection of its prolidase activity is of great interest since it also has two other proline-specific peptidases. Prolyl carboxypeptidase 24 ) was the first studied and has already been characterized, and dipeptidyl peptidase IV is the other, which has recently been cloned (Kabashima et aI., unpublished). The significance of the presence of this battery of enzymes remains to be clarified as well as the detailed study of the prolidase.…”