Prolyl hydroxylase activity in human normal skins and post-burn scars

Hayakawa, Taro; Hino, Masao; Fuyamada, Hiroshi; NAGATSU, T; Aoyama, Hiroko

doi:10.1016/0009-8981(77)90509-5

Cited by 8 publications

(1 citation statement)

References 8 publications

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“…The observed dynamics of GAGs content corresponds to the previously reported data on the concentration of chondroitin-sulfate in the granulation tissue and immature HSs (six times higher than in the skin) and mature scars (slightly higher than in the skin) [6]. The changes in the Hyp/Hyl ratio revealed during the 90 days post-wounding could be attributed both to a shift in the ratio of collagen types [40] and a change in the activity of prolyl and lysyl hydroxylases and lysyl oxidase [38,41]. In the current study, the Hyp/Hyl ratio in scars mostly remained close to the values of normal skin [42] and dropped below the "skin range" only in the first two stages of scar maturation (up to POD 60).…”

Section: Discussionsupporting

confidence: 89%

Modeling of Old Scars: Histopathological, Biochemical and Thermal Analysis of the Scar Tissue Maturation

Fayzullin

Ignatieva

Захаркина

et al. 2021

Biology

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Mature hypertrophic scars (HSs) remain a challenging clinical problem, particularly due to the absence of biologically relevant experimental models as a standard rabbit ear HS model only reflects an early stage of scarring. The current study aims to adapt this animal model for simulation of mature HS by validating the time of the scar stabilization using qualitative and quantitative criteria. The full-thickness skin and perichondrium excision wounds were created on the ventral side of the rabbit ears. The tissue samples were studied on post-operation days (PODs) 30, 60, 90 and 120. The histopathological examination and morphometry were applied in parallel with biochemical analysis of protein and glycosaminoglycans (GAGs) content and amino acid composition. The supramolecular organization of collagen was explored by differential scanning calorimetry. Four stages of the rabbit ear HS maturation were delineated and attributed with the histolomorphometrical and physicochemical parameters of the tissue. The experimental scars formed in 30 days but stabilized structurally and biochemically only on POD 90–120. This evidence-based model can be used for the studies and testing of new treatments of the mature HSs.

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Section: Discussionsupporting

confidence: 89%

Modeling of Old Scars: Histopathological, Biochemical and Thermal Analysis of the Scar Tissue Maturation

Fayzullin

Ignatieva

Захаркина

et al. 2021

Biology

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Human prolyl hydroxylase. Purification, radioimmunoassay and clinical studies in liver diseases

Nagai

Oka

1985

Gastroenterol Jpn

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Prolyl hydroxylase was purified from human placentae, specific antiserum against it was prepared, and a new radioimmunoassay system employing 125I-labelled enzyme preparation was established. The molecular weight of the placental enzyme was shown to be 320,000 by gel filtration. SDS-polyacrylamide gel electrophoresis showed two bands of unequal intensity having molecular weights of 60,000 and 130,000. Their amino acid compositions were identical to each other, suggesting the polypeptide with a molecular weight of 130,000 might be a dimer of the polypeptide with a molecular weight of 60,000. The new radioimmunoassay established had a sensitivity of the order of 10 ng/ml, indicating it was more sensitive than previous radioimmunoassay employing 3H-labelling method. Clinical studies on patients with liver diseases disclosed that the concentrations of serum immunoreactive prolyl hydroxylase were elevated both in cases of hepatocellular damage and in cases of cholestasis. In cases of hepatocellular damage the enzyme behaved like cytoplasmic enzymes such as glutamic oxaloacetic transaminase, glutamic pyruvic transaminase and lactic dehydrogenase, but in cases of cholestasis it resembled biliary enzymes such as alkaline phosphatase and gamma-glutamyl transpeptidase. This result might be associated with the peculiar location of the enzyme within the cell, in the membrane of rough endoplasmic reticulum.

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Enzymes of collagen biosynthesis in skin and serum in dermatological diseases

Kuutti-Savolainen

Kero

1979

Clinica Chimica Acta

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Prolyl hydroxylase activity in human normal skins and post-burn scars

Cited by 8 publications

References 8 publications

Modeling of Old Scars: Histopathological, Biochemical and Thermal Analysis of the Scar Tissue Maturation

Modeling of Old Scars: Histopathological, Biochemical and Thermal Analysis of the Scar Tissue Maturation

Human prolyl hydroxylase. Purification, radioimmunoassay and clinical studies in liver diseases

Enzymes of collagen biosynthesis in skin and serum in dermatological diseases

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