Prolonging the lifetime and activity of silica immobilised Cyanidium caldarium

Rooke, Joanna C.; Vandoorne, Bertrand; Léonard, A.; Meunier, Christophe; Cambier, Pierre; Sarmento, Hugo; Descy, Jean-Pierre; Su, Bao‐Lian

doi:10.1016/j.jcis.2011.01.020

Cited by 13 publications

(13 citation statements)

References 27 publications

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“…About 0.1825 g CaCO 3 /g sodium alginate was added to 5 g (2-3 wt%) sodium alginate solution and homogenized for 1 h [38]. Silica sol obtained from sodium silicate (1.5 M, assay 25.5-28.5%, Merck) was passed over an acid ion exchange resin (Amberlite IR 120, H-form, Acros) [39]. PDADMAC (20 wt% in H 2 O; 30 μL) that catalyzes the polycondensation of the silica precursor was mixed with 5 mL (0.75 M) preformed silicic acid sol and the solution was adjusted to pH 4.8 (±0.1) with NaOH (0.1 M).…”

Section: Synthesis Of Alginate-silica Hybrid Monolithsmentioning

confidence: 99%

Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

Onbas

Yeşil-Çeliktaş

2018

Engineering in Life Sciences

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The organic–inorganic hybrid materials have been used in different fields to immobilize biomolecules since they offer many advantages. The aim of this study was to optimize and characterize the alginate‐silica hybrid hydrogel as a stable and injectable form for microfluidic systems using internal gelation method and increase the stability and activity of immobilized enzyme for biocatalytic conversions as well. Characterization was carried out by scanning electron microscopy, energy dispersive spectroscopy/mapping, Brunauer–Emmett–Teller, Barrett–Joyner–Halenda, and Fourier‐transform infrared spectroscopy analyses, and the shrinkages of monoliths were evaluated. Subsequent to optimizing the enzyme concentration (40 μg), hydrolytic conversion of 4‐nitrophenyl β‐d‐glucopyranoside (pNPG) was performed to understand the behavior of the bioconversion in the microfluidic system. The yield was 94% which reached the equilibrium at 24 h indicating that the alginate‐silica gel derived microsystem overcome some drawbacks of monolithic systems. Additionally, bioconversion of Ruscus aculeatus saponins was carried out at the same setup in order to obtain aglycon part, which has pharmaceutical significance. Although pure aglycon could not be achieved, an intermediate compound was obtained based on the HPLC analysis. The developed formulation can be utilized for various life science applications.

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Section: Synthesis Of Alginate-silica Hybrid Monolithsmentioning

confidence: 99%

Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

Onbas

Yeşil-Çeliktaş

2018

Engineering in Life Sciences

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“…The nucleation of nanoparticulate silica upon the surface of an algal cell wall has already been shown to interfere with the metabolic pathways of the cell. 53,54 Thus this encapsulating hybrid-matrix is more adaptable to the preservation of photosynthetic activity than SiO 2 alone. Fig.…”

Section: Characterisation Techniquesmentioning

confidence: 99%

Green and sustainable production of high value compounds via a microalgae encapsulation technology that relies on CO₂as a principle reactant

et al. 2014

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Title: Green and sustainable production of high value compounds via a microalgae encapsulation technology that relies on CO 2 as a principle reactant This research article highlights the extraordinarily long-term viability and high activity of microalgae entrapped within an alginate-silica hybrid matrix, holding much promise for the design of new generation fi xed biomass photobioreactors that are based on microencapsulation technology. This bionanotechnology opens up exciting avenues for the design of photosynthetic solar cells and artifi cial trees for a green and sustainable production of high value compounds and electricity on the basis of photosynthesis process.A very promising and facile one-pot synthesis pathway is presented for the microencapsulation of live cells in a very porous core-shell system based upon a robust matrix. (Alginate-SiO 2 -polycation)shell@(alginate-SiO 2 ) core hybrid beads, on the millimeter scale, containing live cells are obtained through cross-linking chemistry and the polycondensation of silicic acid in conjunction with the use of a polycation to negate the surface charge on silica. Very interestingly it is revealed that the polycation used (PDADMAC) plays a very important role in the formation of highly robust core-shell beads. PDADMAC acts as a catalyst in the polycondensation of silicic acid, leading to the formation of a resistant double layer shell comprising of an interior layer of alginate-SiO 2 with a very homogeneous distribution of porous SiO 2 and an external layer of porous PDADMAC that confines SiO 2 within the bead. The photosynthetic chlorophyta Dunaliella tertiolecta, which produces high value metabolites (such as antioxidants, pharmacologically active compounds, neutraceuticals etc.) via photosynthesis, has been encapsulated within this core-shell system. Oximetry and fluorescence measurements highlight how this algal culture can remain photosynthetically active over an extraordinarily long period of 13 months for high value compound production, whilst entrapped within a highly porous, mechanically and chemically stable, optically transparent matrix, with no observable leaching of the cells from the core of the beads. HPLC has been employed to highlight the presence of excreted metabolites, based on neutral sugar building blocks such as arabinose, galactose and xylose, in the surrounding media. These results reveal how this kind of high performance, low-cost, and easily scaleable core-shell living material could be employed in large scale photobioreactors (PBRs), to potentially facilitate metabolite harvesting whilst protecting the culture from external contamination and for green energy production and environmental (CO 2 ) remediation.

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“…However the presence of a silica nanopowder (gel B) does not allow light to be transmitted throughout the scaffold, which lends itself to the argument that silica nanoparticles such as Aerosil or Ludox can adversely interfere with visible light. Although productivity results have shown that photosynthetic cells can assimilate carbon dioxide within hybrid materials containing silica nanopowder, [77,88] this would suggest that only the cells found near the surface of the hybrid monolith are able to do so or that scattering effects within the silica matrix enable the light to diffuse through the matrix. Therefore a careful optimization of the matrix is required to balance the positive reinforcing effects with the deleterious influence the nanoparticles have in terms of light absorption or scattering.…”

Section: Optical Transparencymentioning

confidence: 99%

“…Likewise, reports on the recuperation of metabolites would only be possible if diffusion was not severely hindered within the sol-gel matrix. [87,88] One other factor that is related to diffusion is the solubility of carbon dioxide and oxygen within the artificial growth medium. This medium needs to be optimized to levels similar to the natural habitat of the entity it feeds.…”

mentioning

confidence: 99%

Designing Photobioreactors based on Living Cells Immobilized in Silica Gel for Carbon Dioxide Mitigation

et al. 2011

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Atmospheric carbon dioxide levels have been rising since the industrial revolution, with the most dramatic increase occurring since the end of World War II. Carbon dioxide is widely regarded as one of the major factors contributing to the greenhouse effect, which is of major concern in today's society because it leads to global warming. Photosynthesis is Nature's tool for combating elevated carbon dioxide levels. In essence, photosynthesis allows a cell to harvest solar energy and convert it into chemical energy through the assimilation of carbon dioxide and water. Therefore photosynthesis is regarded as an ideal way to harness the abundance of solar energy that reaches Earth and convert anthropologically generated carbon dioxide into useful carbohydrates, providing a much more sustainable energy source. This Minireview aims to tackle the idea of immobilizing photosynthetic unicellular organisms within inert silica frameworks, providing protection both to the fragile cells and to the external ecosystem, and to use this resultant living hybrid material in a photobioreactor. The viability and activity of various unicellular organisms are summarized alongside design issues of a photobioreactor based on living hybrid materials.

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Prolonging the lifetime and activity of silica immobilised Cyanidium caldarium

Cited by 13 publications

References 27 publications

Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

Green and sustainable production of high value compounds via a microalgae encapsulation technology that relies on CO₂as a principle reactant

Designing Photobioreactors based on Living Cells Immobilized in Silica Gel for Carbon Dioxide Mitigation

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Prolonging the lifetime and activity of silica immobilised Cyanidium caldarium

Cited by 13 publications

References 27 publications

Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

Synthesis of alginate‐silica hybrid hydrogel for biocatalytic conversion by β‐glucosidase in microreactor

Green and sustainable production of high value compounds via a microalgae encapsulation technology that relies on CO2as a principle reactant

Designing Photobioreactors based on Living Cells Immobilized in Silica Gel for Carbon Dioxide Mitigation

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Green and sustainable production of high value compounds via a microalgae encapsulation technology that relies on CO₂as a principle reactant