Prolonged treatment with 3-isobutyl-1-methylxanthine improves the efficiency of differentiating 3T3-L1 cells into adipocytes

Hua, Yongjie; Ke, Shanshan; Wang, Yao; Irwin, David M.; Wang, Zhe

doi:10.1016/j.ab.2016.05.007

Cited by 9 publications

(8 citation statements)

References 17 publications

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“…DMI is composed of normal growth media, insulin (1 μg/ml; Sigma), dexamethasone (1 μM; Sigma), and 3-isobutyl-1-methyl-xanthine (0.5 mM; IBMX, Sigma). Cells were then switched to DMII, which includes normal growth medium and insulin (1 μg/ml), from days 5 to 9, after which they were placed back into normal growth medium and refed every 2 days ( 32 ). To induce beige adipocytes, 0.5 μM rosiglitazone (Rosi, Sigma) and 0.5 mM IBMX were added into DMII, and then, the cells were exposed to 10 μM isoproterenol (Iso, Sigma) for 4 hours before harvesting ( 33 , 34 ).…”

Section: Methodsmentioning

confidence: 99%

Molecular signatures and functional analysis of beige adipocytes induced from in vivo intra-abdominal adipocytes

et al. 2018

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Section: Methodsmentioning

confidence: 99%

Molecular signatures and functional analysis of beige adipocytes induced from in vivo intra-abdominal adipocytes

et al. 2018

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“…Increases in the concentration of intracellular cAMP activate the transcription factor CREB through a cascade of protein kinase activation . Furthermore, IBMX has been used to differentiate 3T3 cells to adipocytes through transcription factors activated by the C/EBPβ family of proteins, as expression of the CEBPβ gene is induced directly by IBMX …”

Section: Biochemical Cues In Microenvironment Maintenance For Stem Ce...mentioning

confidence: 99%

The Role of the Microenvironment in Controlling the Fate of Bioprinted Stem Cells

et al. 2020

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The field of tissue engineering and regenerative medicine has made numerous advances in recent years in the arena of fabricating multifunctional, three-dimensional (3D) tissue constructs. This can be attributed to novel approaches in the bioprinting of stem cells. There are expansive options in bioprinting technology that have become more refined and specialized over the years, and stem cells address many limitations in cell source, expansion, and development of bioengineered tissue constructs. While bioprinted stem cells present an opportunity to replicate physiological microenvironments with precision, the future of this practice relies heavily on the optimization of the cellular microenvironment. To fabricate tissue constructs that are useful in replicating physiological conditions in laboratory settings, or in preparation for transplantation to a living host, the microenvironment must mimic conditions that allow bioprinted stem cells to proliferate, differentiate, and migrate. The advances of bioprinting stem cells and directing cell fate have the potential to provide feasible and translatable approach to creating complex tissues and organs. This review will examine the methods through which bioprinted stem cells are differentiated into desired cell lineages through biochemical, biological, and biomechanical techniques.

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“…We thought that different MDI media and insulin media might influence the efficiency of differentiation of SCF and IMF adipocytes, as Chen's study and our study differed in the MDI medium (Chen: 0.5 mM 3-isobutyl-1-methylxanthine, 0.25 µM dexamethasone, 5 mg/L insulin; our study: 0.5 mM 3-isobutyl-1-methylxanthine, 1 µM dexamethasone, 1 mg/L insulin) and insulin medium (Chen: 5 mg/L insulin; our study: 1 mg/L insulin) used for adipogenic induction. All three reagents have different and powerful effects in adipocytes, such as adipogenic differentiation, mitochondrial dysfunction and glucose metabolism [32][33][34][35]. In vivo, insulin responses of these two kinds of adipocytes, SCF and IMF adipocytes, may also differ due to the diverse physiological regulation of metabolic fluxes in iWAT and skeletal muscle [36].…”

Section: Discussionmentioning

confidence: 99%

LKB1 Differently Regulates Adipogenesis in Intramuscular and Subcutaneous Adipocytes through Metabolic and Cytokine-Related Signaling Pathways

Zhou

Nong

et al. 2020

Cells

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Liver kinase B1 (LKB1) plays important and various roles in the differentiation and lipid metabolism of adipocytes. However, the current knowledge of the respective roles of LKB1 in subcutaneous fat (SCF) and intramuscular fat (IMF) adipocytes remains unclear. This study aimed to discover the different regulatory mechanisms of LKB1 in SCF and IMF adipocytes. We found that LKB1 overexpression inhibited adipogenesis in both SCF and IMF adipocytes, and SCF adipocytes were more sensitive to regulation by LKB1. Transcriptomics results showed that IMF adipocytes had many more differentially expressed genes (DEGs) than SCF adipocytes. Pathway analysis of the shared and distinct DEGs revealed that the main adipogenesis mechanism was similar between SCF and IMF adipocytes upon LKB1 overexpression, while regulatory and metabolic signaling pathways, such as MAPK, PPAR signaling pathways, were differently regulated by LKB1. Several cytokine-related pathways were only enriched in LKB1-overexpressing IMF adipocytes. Our study reveals different regulators and signaling pathways between SCF and IMF adipocytes under LKB1 overexpression, which may be potential targets to differentially control SCF and IMF deposition and improve our understanding of the regulatory mechanisms of IMF deposition.

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Prolonged treatment with 3-isobutyl-1-methylxanthine improves the efficiency of differentiating 3T3-L1 cells into adipocytes

Cited by 9 publications

References 17 publications

Molecular signatures and functional analysis of beige adipocytes induced from in vivo intra-abdominal adipocytes

Molecular signatures and functional analysis of beige adipocytes induced from in vivo intra-abdominal adipocytes

The Role of the Microenvironment in Controlling the Fate of Bioprinted Stem Cells

LKB1 Differently Regulates Adipogenesis in Intramuscular and Subcutaneous Adipocytes through Metabolic and Cytokine-Related Signaling Pathways

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