Prolonged food deprivation increases mRNA expression of deiodinase 1 and 2, and thyroid hormone receptor β-1 in a fasting-adapted mammal

Abstract

SUMMARYFood deprivation in mammals is typically associated with reduced thyroid hormone (TH) concentrations and deiodinase content and activity to suppress metabolism. However, in prolonged-fasted, metabolically active elephant seal pups, TH levels are maintained, if not elevated. The functional relevance of this apparent paradox is unknown and demonstrates variability in the regulation of TH levels, metabolism and function in food-deprived mammals. To address our hypothesis that cellular TH-mediated activity is upregulated with fasting duration, we quantified the mRNA expression and protein content of adipose and muscle deiodinase type I (DI1) and type II (DI2), and TH receptor beta-1 (THrβ-1) after 1, 3 and 7 weeks of fasting in northern elephant seal pups (N=5-7 per week). Fasting did not decrease the concentrations of plasma thyroid stimulating hormone, total triiodothyronine (tT 3 ), free T 3 , total thyroxine (tT 4 ) or free T 4 , suggesting that the hypothalamic-pituitary-thyroid axis is not suppressed, but rather maintained during fasting. Mean mRNA expression of adipose DI1 and DI2 increased threefold and fourfold, respectively, and 20-and 30-fold, respectively, in muscle. With the exception of adipose DI1, protein expression of adipose DI2 and muscle DI1 and DI2 increased twofold to fourfold. Fasting also increased adipose (fivefold) and muscle (fourfold) THrβ-1 mRNA expression, suggesting that the mechanisms mediating cellular TH activity are upregulated with prolonged fasting. The data demonstrate a unique, atypical mechanism of TH activity and regulation in mammals adapted to prolonged food deprivation in which the potential responsiveness of peripheral tissues and cellular TH activity are increased, which may contribute to their lipid-based metabolism.Key words: lipid metabolism, reverse T3, seal, thyroxine, triiodothyronine. THE JOURNAL OF EXPERIMENTAL BIOLOGY 4648 concentration in hibernating ground squirrels results from reduced TH metabolism, accompanied by a reduction in nuclear receptors, suggesting that TH function is suppressed despite the increase in circulating levels (i.e. cryptically hyperthyroid) (Magnus and Henderson, 1988a;Magnus and Henderson, 1988b). Such variability in the regulation of TH levels and function in food-deprived mammals demonstrates the diversity of TH physiology during periods of acute or chronic food deprivation, and highlights the necessity to perform more comprehensive studies into the functional relevance of these differences.Northern elephant seals, Mirounga angustirostris (Gill 1866), naturally fast from food and water for up to 3 months while on land (Ortiz et al., 1978; Crocker et al., 1998;Le Boeuf and Laws, 1994), and during the post-weaning fast, oxidation of non-esterified fatty acids (NEFAs) accounts for approximately 95% of the pup's metabolic rate (Ortiz et al., 1978;Viscarra et al., 2012). The fasting metabolism of seals is primarily dependent on lipid oxidation (Crocker et al., 1998;Pramfalk et al., 2011; Crocker et al., 2012a; Crocker et al., 2012...

High fatty acid oxidation capacity and phosphorylation control despite elevated leak and reduced respiratory capacity in northern elephant seal muscle mitochondria

“…During development, weaned pups are known to upregulate expression of UCP2 in muscle (Martinez et al, 2013). While its cellular role is less defined, UCP2 may play a similar role in enhancing fatty acid metabolism and reducing ROS (Brand and Esteves, 2005).…”

Placental Iodothyronine Deiodinases Expression in Pregnant Cows Exposed to Propylthiouracil (Ptu) and Thyroid Axis Activity of their Calves

“…As shown by Dentice and coworkers (2013) [45] D2 performs outer ring deiodination and its expression may be regulated by the concentration of THs. Martinez and coworkers (2013) [46] reported an increased expression of D2 in adipose and muscle tissues in food deprived mammals with decreased thyroid hormones concentrations.…”

Thyroid Hormone Regulation and Insulin Resistance: Insights From Animals Naturally Adapted to Fasting

“…These increases occurred while muscle DI1 and DI2 expressions increased, suggesting that the fast enhances cellular TH-mediated events likely to support the animal's energetic needs as the fast progresses. More recently, our group has shown that DI3 expression levels are regulated epigenetically, since methylation increased in muscle much faster than in adipose (78,79,82). These data suggest a strong inhibition via epigenetic modification of the suppressive mechanisms of cellular metabolism to permissively increase TH-mediated cellular events.…”

“…Intriguingly, unlike most fasting mammals, northern elephant seal pups do not decrease cellular TH-mediated events (80,81,101). Their FIGURE 1.…”

“…Over the course of their postweaning fast, pups develop adipose-specific insulin resistance associated with an increase in TH production, leading to increased cellular TH-mediated responses (79,82,129,130,132,133). In response to a TSH infusion, both T4 and T3 increased in late-fasted pups.…”