Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells

Mytych, Jennifer; Żebrowski, Jacek; Lewińska, Anna; Wnuk, Maciej

doi:10.1007/s12035-016-9688-6

Cited by 96 publications

(70 citation statements)

References 78 publications

(111 reference statements)

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“…To illustrate, a hippocampal neuronal cell model (HT22) was treated with AgNPs, obtaining a decrease in cell viability, oxidative damage and hypermethylation in DNA due to the internalization of AgNPs. These effects in normal cells may be prolonged since harmful impacts remain after AgNP removal [29]. Similar reports were found by Gao, et al, demonstrating that AgNPs can potentially damage mouse embryonic stem cells [30].…”

Section: Silver Nanoparticlessupporting

confidence: 68%

Cytotoxic and Antiproliferative Effects of Nanomaterials on Cancer Cell Lines: A Review

Grijalva¹,

Vallejo-López²,

Salazar³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

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Cell models for the study of antiproliferative and/or cytotoxic properties of engineered nanoparticles are valuable tools in cancer research. Several techniques and methods are readily available for the study of nanoparticles' properties regarding selective toxicity and/or antiproliferative effects. Setting up of those techniques, however, needs to be carefully monitored. Harmonization of the wide range of methods available is necessary for assay comparison and replicability. Although individual or core laboratory capabilities play a role in selection and availability of techniques, data arising from cancer cell models are useful in guiding further research. The variety of cell lines available and the diversity of metabolic routes involved in cell responses make in vitro cell models suitable for the study of the biological effect of nanoparticles at the cell level and a valid approach for further in vivo and clinical studies. The present systematic review looks at the in vitro biological effects of different types of nanoparticles in cancer cell models.

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Section: Silver Nanoparticlessupporting

confidence: 68%

Cytotoxic and Antiproliferative Effects of Nanomaterials on Cancer Cell Lines: A Review

Grijalva¹,

Vallejo-López²,

Salazar³

et al. 2018

Unraveling the Safety Profile of Nanoscale Particles and Materials - From Biomedical to Environmental Applications

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“…As reported earlier, AgNPs modulated HT22 cell cycle and proliferation and induced oxidative stress, which were accompanied by elevated levels of p53 and p21. 86 The cells treated with rGO-Ag alone, TSA alone, or combination of both rGO-Ag and TSA exhibited increased expression of p53, Bax, Bak, caspase-3, and caspase-9, and downregulation of Bcl-2 ( Figure 13). This corroborates with findings from earlier studies which showed that treatment with combination of GO and AgNPs and combination of salinomycin 34,40,87 Combination of TSA and quercetin induced apoptosis through p53 activation in mouse tumors compared to control or cells treated with TSA alone.…”

Section: Rgo-ag and Tsa Cause Mitochondrial Dysfunctionmentioning

confidence: 98%

Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3)

Zhang¹,

Huang²,

Zhang³

et al. 2017

IJN

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Background Recently, there has been much interest in the field of nanomedicine to improve prevention, diagnosis, and treatment. Combination therapy seems to be most effective when two different molecules that work by different mechanisms are combined at low dose, thereby decreasing the possibility of drug resistance and occurrence of unbearable side effects. Based on this consideration, the study was designed to investigate the combination effect of reduced graphene oxide-silver nanoparticles (rGO-AgNPs) and trichostatin A (TSA) in human ovarian cancer cells (SKOV3). Methods The rGO-AgNPs were synthesized using a biomolecule called lycopene, and the resultant product was characterized by various analytical techniques. The combination effect of rGO-Ag and TSA was investigated in SKOV3 cells using various cellular assays such as cell viability, cytotoxicity, and immunofluorescence analysis. Results AgNPs were uniformly distributed on the surface of graphene sheet with an average size between 10 and 50 nm. rGO-Ag and TSA were found to inhibit cell viability in a dose-dependent manner. The combination of rGO-Ag and TSA at low concentration showed a significant effect on cell viability, and increased cytotoxicity by increasing the level of malondialdehyde and decreasing the level of glutathione, and also causing mitochondrial dysfunction. Furthermore, the combination of rGO-Ag and TSA had a more pronounced effect on DNA fragmentation and double-strand breaks, and eventually induced apoptosis. Conclusion This study is the first to report that the combination of rGO-Ag and TSA can cause potential cytotoxicity and also induce significantly greater cell death compared to either rGO-Ag alone or TSA alone in SKOV3 cells by various mechanisms including reactive oxygen species generation, mitochondrial dysfunction, and DNA damage. Therefore, this combination chemotherapy could be possibly used in advanced cancers that are not suitable for radiation therapy or surgical treatment and facilitate overcoming tumor resistance and disease progression.

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“…Accumulating evidence suggests that AgNP-mediated ER stress is responsible for cellular dysfunction and activation of the cell death pathway. We summarize the cellular effects of AgNPs in various cellular systems based on exposure doses, concentration, size of particles, type cell line used, and major outcome of each study in Table 1 [24,38,64,72,105,137,138,139,140,141,142,143,144,145,146,147,148]. …”

Section: Activation Of Signaling Molecules In Response To Agnps Inmentioning

confidence: 99%

Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in Vitro Model

Zhang

Shen

Gurunathan

2016

IJMS

221

129

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Silver nanoparticles (AgNPs) have attracted increased interest and are currently used in various industries including medicine, cosmetics, textiles, electronics, and pharmaceuticals, owing to their unique physical and chemical properties, particularly as antimicrobial and anticancer agents. Recently, several studies have reported both beneficial and toxic effects of AgNPs on various prokaryotic and eukaryotic systems. To develop nanoparticles for mediated therapy, several laboratories have used a variety of cell lines under in vitro conditions to evaluate the properties, mode of action, differential responses, and mechanisms of action of AgNPs. In vitro models are simple, cost-effective, rapid, and can be used to easily assess efficacy and performance. The cytotoxicity, genotoxicity, and biocompatibility of AgNPs depend on many factors such as size, shape, surface charge, surface coating, solubility, concentration, surface functionalization, distribution of particles, mode of entry, mode of action, growth media, exposure time, and cell type. Cellular responses to AgNPs are different in each cell type and depend on the physical and chemical nature of AgNPs. This review evaluates significant contributions to the literature on biological applications of AgNPs. It begins with an introduction to AgNPs, with particular attention to their overall impact on cellular effects. The main objective of this review is to elucidate the reasons for different cell types exhibiting differential responses to nanoparticles even when they possess similar size, shape, and other parameters. Firstly, we discuss the cellular effects of AgNPs on a variety of cell lines; Secondly, we discuss the mechanisms of action of AgNPs in various cellular systems, and try to elucidate how AgNPs interact with different mammalian cell lines and produce significant effects; Finally, we discuss the cellular activation of various signaling molecules in response to AgNPs, and conclude with future perspectives on research into AgNPs.

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Prolonged Effects of Silver Nanoparticles on p53/p21 Pathway-Mediated Proliferation, DNA Damage Response, and Methylation Parameters in HT22 Hippocampal Neuronal Cells

Cited by 96 publications

References 78 publications

Cytotoxic and Antiproliferative Effects of Nanomaterials on Cancer Cell Lines: A Review

Cytotoxic and Antiproliferative Effects of Nanomaterials on Cancer Cell Lines: A Review

Novel biomolecule lycopene-reduced graphene oxide-silver nanoparticle enhances apoptotic potential of trichostatin A in human ovarian cancer cells (SKOV3)

Silver Nanoparticle-Mediated Cellular Responses in Various Cell Lines: An in Vitro Model

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