Prolonged decrease in hepatic connexin32 in chronic liver injury induced by carbon tetrachloride in rats

Nakata, Yutaka; Iwai, Masaru; Kimura, Shigeru; Shimazu, Takashi

doi:10.1016/s0168-8278(96)80213-3

Cited by 33 publications

(34 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Since both liver development and cancer are associated with clear-cut changes in connexin expression, these proteins may form a novel set of clinical diagnostic indicators and therapeutic targets (Nakashima et al, 2004;Nakata et al, 1996;Yamaoka et al, 2000). Specifi cally, connexins could be used as biomarkers of disease.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Modifications in Connexin Expression in Liver Development and Cancer

Vinken

Kock

Oliveira

et al. 2012

Cell Communication & Adhesion

View full text Add to dashboard Cite

The establishment of an elaborate gap junctional intercellular communication network, especially between hepatocytes, is important for normal liver development. In fact, the production of the gap junction building blocks, the connexins, undergoes several well-defi ned changes throughout the hepatic differentiation process. This ultimately results in the acquisition of an adult connexin expression pattern which is critical for maintaining the fully differentiated hepatocyte-specifi c phenotype. Abnormalities of connexin production are observed in a number of pathological conditions, such as during liver cancer. This article provides an overview of these processes with emphasis on the underlying molecular mechanisms.

show abstract

Section: Discussionmentioning

confidence: 99%

“…hepatitis, fi brosis and cirrhosis) as well as during hepatotoxicity (Nakashima et al, 2004;Nakata et al, 1996;Yamaoka et al, 2000). This is equally refl ected at the posttranscriptional level and is caused by increased degradation of Cx32 mRNA molecules (Gingalewski et al, 1996).…”

Section: Connexins and Alterations In The Liver Differentiation Statusmentioning

confidence: 99%

Modifications in Connexin Expression in Liver Development and Cancer

Vinken

Kock

Oliveira

et al. 2012

Cell Communication & Adhesion

View full text Add to dashboard Cite

show abstract

“…Recently, in vitro studies verified that Cxs32 can be expressed without forming gap junctions, being accumulated in the cytoplasm, if their intracellular transport mechanism is deficient (Hernandez-Blázquez et al 2001) or in the absence of E-cadherin (Yano et al 2001). That could explain the atypical plaques and the increase of mRNA without increase of gap junctions described by Nakata et al (1996). The reason for this difference in location is not clear, but it may be due to the fact that in the liver, post-lesion regenerative processes multiply and decrease intercellular communication mediated by gap junctions.…”

Section: Discussionmentioning

confidence: 96%

Expression and distribution of connexin 32 in rat liver with experimentally induced fibrosis

et al. 2009

View full text Add to dashboard Cite

RESUMO.-[Expressão e distribuição da conexina 32 em fígados de ratos com fibrose induzida experimentalmente.] A conexina 32 (Cx32) é uma proteína que constitui os canais que promovem as comunicações intercelulares via junções comunicantes (CIJC) no fígado, permitindo difusão de pequenas moléculas citoplasmáticas de uma célula à outra. A fibrose hepática caracteriza-se pela alteração da arquitetura normal do fígado e podem alterar as CIJCs. O objetivo deste trabalho foi estudar a expressão e distribuição de Cx32 na fibrose hepática. O objetivo do presente trabalho foi estudar a expressão e distribuição da Cx32 em fígados com fibrose induzida pela administração oral de dimetilnitrosamina em fêmeas de ratos Wistar. A necropsia foi realizada após cinco semanas da última administração da droga e observou-se um quadro de fibrose hepática. Amostras dos fígados com fibrose e de animais controle foram submetidas à análise imunoistoquímica, por Real Time-PCR e por Western-Blot verificando-se a presença de Cx32 difusa e dispersa no citoplasma dos fígados com fibrose. No grupo controle a Cx32 localizou-se na membrana citoplasmática com a formação de placas juncionais. O fígado com fibrose também revelou diminuição da expressão gênica de Cx32, embora sem a redução da quantidade do produto protéico, quando comparado ao grupo controle. Estes resultados suge- (Cx32) is a protein that forms the channels that promote the gap junction intercellular communication (GJIC) in the liver, allowing the diffusion of small molecules through cytosol from cell-to-cell. Hepatic fibrosis is characterized by a disruption of normal tissue architeture by cellular lesions, and may alter the GJIC. This work aimed to study the expression and distribution of Cx32 in liver fibrosis induced by the oral administration of dimethylnitrosamine in female Wistar rats. The necropsy of the rats was carried out after five weeks of drug administration. They presented a hepatic fibrosis state. Sections from livers with fibrosis and from control livers were submitted to immunohistochemical, Real Time-PCR and Western-Blot analysis to Cx32. In fibrotic livers the Cxs were diffusely scattered in the cytoplasm, contrasting with the control livers, where the Cx32 formed junction plaques at the cell membrane. Also it was found a decrease in the gene expression of Cx32 without reduction in the protein quantity when compared with controls. These results suggest that there the mechanism of intercellular communication between hepatocytes was reduced by the fibrotic process, which may predispose to the occurrence of a neoplastic process, taken in account that connexins are considered tumor suppressing genes. rem que o mecanismo de comunicação intercelular entre os hepatócitos reduziu-se durante o processo fibrótico, o que pode predispor a ocorrência de processos neoplási-cos, uma vez que as conexinas são consideradas genes supressores de tumores.

show abstract

“…Em cirroses hepáticas e hepatites virais crônicas, o número de junções comunicantes também se mostrou menor que o observado em fígados normais (NAKATA et al, 1996;YAMAOKA, 1999). Nakata et al (1996) observaram que ocorre redução da Cx32 em fígados com lesão crônica induzida por tetracloreto de carbono, embora tivessem notado um aumento de seu mRNA e imagens de placas atípicas.…”

Section: Ctgfunclassified

Expressão e distribuição da conexina 32 em fígados com fibrose experimentalmente induzida

Rodrigues¹

View full text Add to dashboard Cite

Prolonged decrease in hepatic connexin32 in chronic liver injury induced by carbon tetrachloride in rats

Cited by 33 publications

References 32 publications

Modifications in Connexin Expression in Liver Development and Cancer

Modifications in Connexin Expression in Liver Development and Cancer

Expression and distribution of connexin 32 in rat liver with experimentally induced fibrosis

Expressão e distribuição da conexina 32 em fígados com fibrose experimentalmente induzida

Contact Info

Product

Resources

About