Prolonged controlled delivery of nerve growth factor using porous silicon nanostructures

Zilony, Neta; Rosenberg, Michal; Holtzman, Liran; Schori, Hadas; Shefi, Orit; Segal, Ester

doi:10.1016/j.jconrel.2016.12.008

Cited by 41 publications

(20 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nanostructured materials have emerged as promising candidates for drug delivery, especially for cancer treatment 1 . Apart from the conventional polymer- and lipid-based drug delivery systems (DDS) and other inorganic nanomaterials 2–5 , porous Si (pSi) is an attractive material for nanomedicine applications due to its remarkable properties such as the large surface area (up to 800 m 2 ·g −1 ) 6 , biocompatibility 7–9 and biodegradability 10–12 while retaining drug bioactivity 13 . In addition, nanostructured pSi has shown unique optical 14 and luminescent properties 11,15 , which are conducive to self-reporting drug loading and release.…”

Section: Introductionmentioning

confidence: 99%

Spatially Controlled Surface Modification of Porous Silicon for Sustained Drug Delivery Applications

Zhang

Yoshikawa

Welch

et al. 2019

Sci Rep

View full text Add to dashboard Cite

A new and facile approach to selectively functionalize the internal and external surfaces of porous silicon (pSi) for drug delivery applications is reported. To provide a surface that is suitable for sustained drug release of the hydrophobic cancer chemotherapy drug camptothecin (CPT), the internal surfaces of pSi films were first modified with 1-dodecene. To further modify the external surface of the pSi samples, an interlayer was applied by silanization with (3-aminopropyl)triethoxysilane (APTES) following air plasma treatment. In addition, copolymers of N-(2-hydroxypropyl) acrylamide (HPAm) and N-benzophenone acrylamide (BPAm) were grafted onto the external pSi surfaces by spin-coating and UV crosslinking. Each modification step was verified using attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, water contact angle (WCA) measurements, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). In order to confirm that the air plasma treatment and silanization step only occurred on the top surface of pSi samples, confocal microscopy was employed after fluorescein isothiocyanate (FITC) conjugation. Drug release studies carried out over 17 h in PBS demonstrated that the modified pSi reservoirs released CPT continuously, while showing excellent stability. Furthermore, protein adsorption and cell attachment studies demonstrated the ability of the graft polymer layer to reduce both significantly. In combination with the biocompatible pSi substrate material, the facile modification strategy described in this study provides access to new multifunctional drug delivery systems (DDS) for applications in cancer therapy.

show abstract

Section: Introductionmentioning

confidence: 99%

Spatially Controlled Surface Modification of Porous Silicon for Sustained Drug Delivery Applications

Zhang

Yoshikawa

Welch

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Many groups, including ours, have developed various non-magnetic strategies for local delivery of NGF. Nanostructured porous silicon chips were synthesized for prolonged controlled release of NGF for localized treatment [ 38 ]. Likewise, fibered nerve conduits with spatial gradients of neurotrophic factors were shown to release NGF locally at the injured site [ 39 ].…”

Section: Introductionmentioning

confidence: 99%

Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles

et al. 2018

Self Cite

View full text Add to dashboard Cite

Growth factors play an important role in nerve regeneration and repair. An attractive drug delivery strategy, termed “magnetic targeting”, aims to enhance therapeutic efficiency by directing magnetic drug carriers specifically to selected cell populations that are suitable for the nervous tissues. Here, we covalently conjugated nerve growth factor to iron oxide nanoparticles (NGF-MNPs) and used controlled magnetic fields to deliver the NGF–MNP complexes to target sites. In order to actuate the magnetic fields a modular magnetic device was designed and fabricated. PC12 cells that were plated homogenously in culture were differentiated selectively only in targeted sites out of the entire dish, restricted to areas above the magnetic “hot spots”. To examine the ability to guide the NGF-MNPs towards specific targets in vivo, we examined two model systems. First, we injected and directed magnetic carriers within the sciatic nerve. Second, we injected the MNPs intravenously and showed a significant accumulation of MNPs in mouse retina while using an external magnet that was placed next to one of the eyes. We propose a novel approach to deliver drugs selectively to injured sites, thus, to promote an effective repair with minimal systemic side effects, overcoming current challenges in regenerative therapeutics.

show abstract

“…Presence of the prepared samples (CDs and E@CDs) cells showed retention of the ability of differentiation, such as neurite outgrowth and the complex network formation. Possible reason explained somewhere else . At different time points – 1, 3 and 6 days, through imaging the progress of differentiation of cell was monitored.…”

Section: Resultsmentioning

confidence: 99%

One‐Pot Hydrothermal Synthesis of Elements (B, N, P)‐Doped Fluorescent Carbon Dots for Cell Labelling, Differentiation and Outgrowth of Neuronal Cells

Kumar

Friedman

et al. 2019

ChemistrySelect

Self Cite

View full text Add to dashboard Cite

Here, we report the use of the chemically modified one-step facile hydrothermal process to develop Boron (B), Nitrogen (N), and Phosphorous (P) doped carbon dots (E@CDs). The chemical characterization of the E@CDs was systematically studied by several analytical techniques that confer doping of elements by known characteristics. The obtained E@CDs had observed very homogeneous size distribution and displayed excitation dependent fluorescence properties, and high quantum yield (QY) was measured in case of B and N doping. Excellent cell viability and good cellular uptake was observed for all E@CDs. Finally, E@CDs were compared with pristine CDs on their effect on cell labelling, neural differentiation process and outgrowth of neurite network. By manipulating the doping of CDs, we can control the branching pattern and outgrowth of neuronal developments. The E@CDs are capable due to their photostability biocompatibility, and possible selective affinity towards nanomedicine applications.[a] Dr.

show abstract

Prolonged controlled delivery of nerve growth factor using porous silicon nanostructures

Cited by 41 publications

References 74 publications

Spatially Controlled Surface Modification of Porous Silicon for Sustained Drug Delivery Applications

Spatially Controlled Surface Modification of Porous Silicon for Sustained Drug Delivery Applications

Magnetic Targeting of Growth Factors Using Iron Oxide Nanoparticles

One‐Pot Hydrothermal Synthesis of Elements (B, N, P)‐Doped Fluorescent Carbon Dots for Cell Labelling, Differentiation and Outgrowth of Neuronal Cells

Contact Info

Product

Resources

About