Prolonged caffeine intake decreases alveolar bone damage induced by binge-like ethanol consumption in adolescent female rats

Binge‐Like Exposure During Adolescence Induces Detrimental Effects in Alveolar Bone that Persist in Adulthood

“…Although this binge‐like EtOH protocol was used in adult subjects, with reduced alveolar bone quality, alveolar bone loss was not observed (Frazão et al, 2020). Early microstructure changes already have been reported by our group, as reduced trabecular thickness, increased trabecular separation and decreased bone volume in the alveolar bone of rats exposed to 4 cycles of binge‐like EtOH exposure (Maia et al, 2020). The alveolar bone compound supports periodontal tissue and plays important role to the oral health maintenance, in which provides support for the teeth and dissipates chewing forces.…”

“…The central portion of the furcation region (interradicular area) of the inferior first molar was chosen as the region of interest (ROI) for analysis that was performed in an area of 0.2mm 2 , as shown in Fig. 2 A (Maia et al, 2020).…”

“…The height of the peaks was used to calculate the ratio. The average values were used to describe the overall mineral:matrix ratio, and the average values in each analyzed point were used to plot the mineral:matrix ratio in the area (Maia et al, 2020).…”

The Role of the Adenosine System on Emotional and Cognitive Disturbances Induced by Ethanol Binge Drinking in the Immature Brain and the Beneficial Effects of Caffeine

“…We established and validated a new protocol of chronic caffeine treatment (10 mg/kg for 21 days) in adolescent female animals submitted to a binge drinking ethanol challenge. Firstly, we investigated alveolar bone homeostasis, in which caffeine prevented ethanol bone loss that was not mediated by A2AR blockade [14]. Presently, we are testing this protocol in an anxiety-like model, assessing new strategies of pharmacological manipulations as therapeutic tools, i.e., agonism of A1R and antagonism of A2AR.…”

“…For instance, we demonstrated that physical exercise is a useful tool to attenuate or prevent ethanol damage [13]. Moreover, we demonstrated that caffeine is able to prevent ethanol-induced alveolar bone loss in adolescent rats [14]. We further explored the effects of caffeine on the CNS in view of the known beneficial effects of the regular intake of moderate doses of caffeine [15], in particular, to attenuate neurotoxicity in different animal models of brain diseases [16,17].…”