Prolonged bioluminescence imaging in living cells and mice using novel pro-substrates for<i>Renilla</i>luciferase

Yuan, Mingliang; Ma, Xiaojie; Jiang, Tianyu; Gao, Yuqi; Cui, Yuanyuan; Zhang, Chaochao; Yang, Xiande; Huang, Yun; Du, Lüpei; Yampolsky, Ilia V.; Li, Minyong

doi:10.1039/c7ob01656e

Cited by 14 publications

(8 citation statements)

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“…The brightness and tissue penetration of the bioluminescent signal has limited its wide application in vivo. In previous studies 12,[21][22][23] , nearly all in vivo cell-tracking experiments with bioluminescence used a small animal (mouse or zebrafish, etc.) as research model.…”

Section: Discussionmentioning

confidence: 99%

“…Increasing bioluminescence brightness intensity is a trend in the associated research. Yuan et al 21 designed and synthesized pro-substrates to enhance the improved light signal, and found that butyryloxymethyl luciferin 7 can serve as a promising long-term bioluminescence imaging probe. Gregor et al 12 reported the generation of an improved bacteria luciferase with an approximately 7-fold increased brightness.…”

Section: Discussionmentioning

confidence: 99%

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Non-Invasive Cell Tracking with Brighter and Red-Transferred Luciferase for Potential Application in Stem Cell Therapy

Dou

Matz

et al. 2019

Cell Transplant

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This study investigated the safety of a novel cell-labeling technology with mKATE and Renilla reniformis luciferase (mKATE-renLUC) and assessed the efficacy on tracking implanted human placental stromal cells (PSC) in an erectile dysfunction (ED) animal model. Human PSC were labeled with mKATE-renLUC using a lentivirus. Cell viability, apoptosis, proliferation, migration, surface marker expression and differentiation potential of the labeled PSC were evaluated and compared with non-labeled PSC. The paracrine profile of labeled cells was examined using an angiogenesis protein array. The brightness and duration of labeled cells with different densities were evaluated. An ED rat model was established and labeled PSC were injected into cavernosal tissue of the penis. The migration and distribution of transplanted PSC were monitored using an IVIS imaging system in real time. Implanted PSC were identified in isolated tissues via detection of mKATE fluorescence. The cell viability, morphology, proliferation, migration, surface marker expression and differentiation potential of mKATE-renLUC-labeled PSC were similar to those of non-labeled cells in vitro (no statistical difference p>0.05). Similar expressions of trophic factors were found between labeled and non-labeled PSC. The migration and distribution of PSC expressing renLUC were tracked in vivo using IVIS imaging system. mKATE-positive PSC were detected in penile, kidney, prostate and hepatic tissues using histological methods. This labeling technology provides a safe and effective cell-tracking approach with a brighter fluorophore and codon-optimized luciferase.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Non-Invasive Cell Tracking with Brighter and Red-Transferred Luciferase for Potential Application in Stem Cell Therapy

Dou

Matz

et al. 2019

Cell Transplant

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“…The designed luminescent pairs provide more selections, and these luciferin analogs might be able to be biosynthesized in plant cells. Given that the potential cytotoxicity and growth inhibition to higher plants caused by bioluminescent systems, protecting groups were introduced into luciferin, which can decrease cytotoxicity and enhance the accumulation of luciferin (Yuan et al, 2017).…”

Section: Optimization Of “Planterns”mentioning

confidence: 99%

Glowing plants can light up the night sky? A review

Chen

Zhu

et al. 2021

Biotech & Bioengineering

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Luminescence, a physical phenomenon that producing cool light in vivo, has been found in bacteria, fungi, and animals but not yet in terrestrial higher plants. Through genetic engineering, it is feasible to introduce luminescence systems into living plant cells as biomarkers. Recently, some plants transformed with luminescent systems can glimmer in darkness, which can be observed by our naked eyes and provides a novel lighting resource. In this review, we summarized the bioassay development of luminescence in plant cells, followed by exampling the successful cases of glowing plants transformed with diverse luminescent systems. The potential key factors to design or optimize a glowing plant were also discussed. Our review is useful for the creation of the optimized glowing plants, which can be used not only in scientific research, but also as promising substitutes of artificial light sources in the future.

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“…Instead, the effect of DMSO in the hydrolytic mechanism should be considered, as it is itself responsible for drastic changes compared to what has been observed in aqueous solutions (27). Yuan and coworkers (29) report an opposite hydrolytic trend of derivative 4a dissolved in water, which is necessarily related to solvent change. Literature suggests that organic carboxylic acids show an increasing value of pKa (as example, acetic acid from 4.76 to 12.3) when dissolved in DMSO (30), which means the protonated form of the acids is more stable in this solvent.…”

Section: Kinetic Studiesmentioning

confidence: 99%

Stability Studies of New Caged bis‐deoxy‐coelenterazine Derivatives and Their Potential Use as Cellular pH Probes

Giuliani

Merolla

Paolino

et al. 2020

Photochem & Photobiology

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The synthesis of new bis‐deoxy‐coelenterazine (1) derivatives bearing ester protective groups (acetate, propionate and butyrate esters) was accomplished. Moreover, their hydrolytic stability at room temperature was evaluated in dimethylsulfoxide (DMSO) as solvent, using the nuclear magnetic resonance (NMR) spectra of the key products at different time intervals. The results showed an increasing hydrolysis rate according to longest aliphatic chain, with a half‐life of 24 days of the more stable acetate derivative (4a). Furthermore, the analysis of the experimental data revealed the greater stability of the enol tautomer in this aprotic polar solvent. This result was confirmed by theoretical calculations using the density functional theory (DFT) approach, which gave us the opportunity to propose a detailed decomposition mechanism. Additionally, the derivatives obtained were tested by bioluminescence luciferase assays to evaluate their potential use as extracellular pH‐sensitive reporter substrates of luciferase. The biological data support the idea that further structural modifications of these molecules may open promising perspectives in this field of research.

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Prolonged bioluminescence imaging in living cells and mice using novel pro-substrates forRenillaluciferase

Cited by 14 publications

References 50 publications

Non-Invasive Cell Tracking with Brighter and Red-Transferred Luciferase for Potential Application in Stem Cell Therapy

Non-Invasive Cell Tracking with Brighter and Red-Transferred Luciferase for Potential Application in Stem Cell Therapy

Glowing plants can light up the night sky? A review

Stability Studies of New Caged bis‐deoxy‐coelenterazine Derivatives and Their Potential Use as Cellular pH Probes

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