Prolonged and highly efficient intracellular extraction of photosynthetic electrons from single algal cells by optimized nanoelectrode insertion

Hong, Hyeonaug; Kim, Yong Jae; Han, Myungjin; Yoo, Gu; Song, Hyun Woo; Chae, Youngcheol; Pyun, Jae Chul; Grossman, Arthur R.; Ryu, Wooseok

doi:10.1007/s12274-017-1642-z

Cited by 18 publications

(10 citation statements)

References 38 publications

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“…For microalgal injection, the shape, dimensions, and density of the template pores must be carefully selected to enable the resulting needles to penetrate through the cell‐wall/cell‐membrane dual barrier without breaking or bending, while also minimizing cell death. For C. reinhardtii cells, immediate cell rupture upon impalement have been reported for needles with tips that are larger than 900 nm . We have also found that complete skewering of the C. reinhardtii induce immediate cell death, and when the needle density is too high ( e. g .…”

Section: Template Synthesis Of Nna For C Reinhardtii Impalement and supporting

confidence: 64%

Template‐Synthesized Vertical Needle Array as Injection Platform for Microalgae

Mukaibo

2018

The Chemical Record

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Increasing numbers of studies in the past few decades have demonstrated vertically‐oriented nanoneedles arrays (NNAs) as innovative tools to interrogate and manipulate biological cells, where the needles are inserted into the cells as functional probes for high‐throughput detection and biomolecule delivery. However, majority of these studies use mammalian cells: leaving NNA application to plant cells still in its infancy and largely unexplored. This paper highlights our contributions in exploring the utility of NNAs to microalgae – a diverse group of aquatic, photosynthetic organisms studied intensively as bio‐factories for producing high‐value‐added products such as fuels and pharmaceuticals. Microalgal strain development processes have long suffered from the hard cell wall that surrounds the cell and inhibits delivery of foreign materials into the cell. Conically‐shaped, metallic NNAs were developed with template synthesis that successfully penetrate through the cell wall barrier and achieve material injection – using the widely studied model microalga, Chlamydomonas reinhardtii. Earlier works from mammalian cells are introduced and discussed to clarify the framework established in this field, while recent studies of both mammalian and microalgal cells are also referenced to examine the trends, challenges, and future perspectives of NNA application to microalgae.

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Section: Template Synthesis Of Nna For C Reinhardtii Impalement and supporting

confidence: 64%

Template‐Synthesized Vertical Needle Array as Injection Platform for Microalgae

Mukaibo

2018

The Chemical Record

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“…For examples, the photosynthetic energy harvesting from algal cells was successfully demonstrated for 7 days. 10,11 In the electron transfer process of photosynthesis, reactive oxygen species (ROS) are produced and damage the isolated photosynthetic apparatuses such as PS I and PS II or TMs. 43−45 Thus, PBECs using isolated TMs are vulnerable to ROS damage and typically show rapid degeneration of their performance.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies demonstrated the possibility of sustainable PBEC from the extraction of PEs during 7 days. 10,11 Although the whole-cell based PBECs have advantages such as having no need for an isolation process and long-duration operation, there are major drawbacks of limited scalability or need for mediators. As another approach, use of subcellular photosynthetic apparatus such as PS II or PS I, PE current density of up to ∼410 μA/cm 2 was achieved.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, TM-based PE harvesting has also been extensively studied. For effective transfer and collection of PE in TM biophotoanodes, PEs were extracted with the assistance of a soluble redox-mediator such as ferricyanide (K (CN) 6 ), 17,21,25 quinones, 26,27 and osmium redox polymer ( [ O s ( 2 , 2 ′ -b i p y r i d i n e ) 2 -p o l y ( N -v i n y l i m i d a z ole) 10 Cl] +/+2 ), 18,19,23,24 or nanomaterials such as multiwalled carbon nanotubes (MWCNTs) 19,21,26 and graphene oxide (GO). 17,25,28 Large PE current densities were achieved, up to a few hundred μA/cm 2 .…”

Section: Introductionmentioning

confidence: 99%

“…These electrons, photosynthetic electrons (PEs), are excited to high energy states by solar energy and electrochemically transferred from PS II to photosystem I (PS I) through the transmembrane proteins (e.g., plastoquinone pools, Cytochrome b 6 f complexes) in electron transfer chain (ETC) . To extract the high energy PEs, various investigations were made with the whole cells such as cyanobacteria , and algal cells, − and the cellular or the subcellular photosynthetic apparatuses such as PS I, , PS II, , and TMs. − Extraction of PEs from the single or multiple algal cells were investigated by nanoelectrode insertion. Recent studies demonstrated the possibility of sustainable PBEC from the extraction of PEs during 7 days. , Although the whole-cell based PBECs have advantages such as having no need for an isolation process and long-duration operation, there are major drawbacks of limited scalability or need for mediators.…”

Section: Introductionmentioning

confidence: 99%

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Electrosprayed Thylakoid–Alginate Film on a Micro-Pillar Electrode for Scalable Photosynthetic Energy Harvesting

Kim

Hong

et al. 2020

ACS Appl. Mater. Interfaces

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Direct harvesting of electricity from photosynthesis is highly desired as an eco-friendly and sustainable energy harvesting technology. Photosynthetic apparatuses isolated from plants, such as thylakoid membranes (TMs), are deposited on an electrode by which photosynthetic electrons (PEs) are collected from water splitting. To enhance PE collection efficiency, it is critical to increase the electrochemical interfaces between TMs and the electrode. Considering the size of TMs to be around a few hundred nanometer, we hypothesize that an array of micropillar-shaped (MP) electrode can maximize the TM/ electrode interface area. Thus, we developed MP electrodes with different heights and investigated the electrospraying of TM−alginate mixtures to fill the gaps between MPs uniformly and conformally. The uniformity of the TM−alginate film and the interaction between the TM and the MP electrode were evaluated to understand how the MP heights and film quality influenced the magnitude of the PE currents. PE currents increased up to 2.4 times for an MP electrode with an A/R of 1.8 compared to a flat electrode, indicating increased direct contact interface between TMs and the electrode. Furthermore, to demonstrate the scalability of this approach, an array of replicated SU-8 MP electrodes was prepared and PE currents of up to 3.2 μA were monitored without a mediator under 68 mW/cm 2 . Finally, the PE current harvesting was sustained for 14 days without decay, demonstrating the long-term stability of the TM−alginate biophotoanodes.

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Photosynthetic Nanomaterial Hybrids for Bioelectricity and Renewable Energy Systems

et al. 2020

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How PE harvesting systems have improved concerning solar energy absorption, PE production, and PE collection by electrodes is discussed. The review focuses on how different kinds of nanomaterials are applied and function in interfacing with photosynthetic materials for enhanced PE harvesting. Finally, the review analyzes how the performance of PE harvesting and stand-alone systems have evolved so far and its future prospects.

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Prolonged and highly efficient intracellular extraction of photosynthetic electrons from single algal cells by optimized nanoelectrode insertion

Cited by 18 publications

References 38 publications

Template‐Synthesized Vertical Needle Array as Injection Platform for Microalgae

Template‐Synthesized Vertical Needle Array as Injection Platform for Microalgae

Electrosprayed Thylakoid–Alginate Film on a Micro-Pillar Electrode for Scalable Photosynthetic Energy Harvesting

Photosynthetic Nanomaterial Hybrids for Bioelectricity and Renewable Energy Systems

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