Proline transport by Pseudomonas aeruginosa

Kay, William W.; Gronlund, Audrey F.

doi:10.1016/0005-2736(69)90203-x

Cited by 37 publications

(23 citation statements)

References 19 publications

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“…We found a good correlation between the rate of Lmethionine incorporation rates and the growth rates of cultures, irrespective of the strain or induction of the culture. This finding is in agreement with earlier reports, where the total amino acid pool levels for Pseudornonas and E. coli were found to be similar during exponential growth, although the composition of the pools differed slightly with respect to the individual amino acids (Britten and McClure, 1962;Kay and Gronlund, 1969). However, the L-methionine incorporation rates observed during the pulselchase labelings were consistently fourfold lower than the theoretical L-methionine incorporation rate needed to support a given growth rate, based on chemical composition data for E. coli Fig.…”

Section: Incorporation Of [35s]methionine Into Total Proteinsupporting

confidence: 94%

The AlkB Monooxygenase of Pseudomonas oleovorans

Staijen

Hatzimanikatis

1997

European Journal of Biochemistry

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We have studied the synthesis and stability of the monooxygenase AlkB of Pseudomonas oleovorans in its natural host and in recombinant Escherichia coli. Three strains were investigated: the prototype strain F! oleovorans and the E. coli alk' recombinants HBlOl (pGEc47) and W3110 (pGEc47). Plasmid pGEc47 allows regulated expression of alkB and synthesis of active AlkB in E. coli.The E. coli strains were selected because E. coli HBlOl (pGEc47) produces similar amounts of AlkB as I? oleovorans (1.5-2% of total cell protein), whereas E. coli W3110 (pGEc47) is able to make substantially (about fivefold) more AlkB. The AlkB synthesis and degradation rates in batch cultures of the three strains were determined by means of isotopic-labeling and immunological techniques. The mean specific AlkB synthesis rates in F! oleovorans, E. coli HBlOl (pGEc47) and E. coli W3110 (pGEc47) were approximately 7, 12.5 and 45 pg . mg protein-' . h-', respectively. The half-lives of AlkB were estimated to be 80, 3 and 15 for l? oleovorans, E. coli HBlOl (pGEc47) and E. coli W3110 (pGEc47), respectively. Thus, the intracellular AlkB level in each of the three strains was the result of their AlkB synthesis and degradation rates. The AlkB level during batch growth was modelled by means of experimentally derived parameters for AlkB synthesis and degradation, and showed good agreement with AlkB levels determined by means of immunoblotting in all strains investigated.

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Section: Incorporation Of [35s]methionine Into Total Proteinsupporting

confidence: 94%

The AlkB Monooxygenase of Pseudomonas oleovorans

Staijen

Hatzimanikatis

1997

European Journal of Biochemistry

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“…The factors involved in germination are diverse and much still needs to be done to sort out seemingly conflicting observations. Inducible proline transport systems have been described in both bacteria and yeasts (Jayakumar et al, 1978(Jayakumar et al, , 1979Kay & Gronlund, 1969;Schwencke & Magaiia-Schwencke, 1979). The kinetics of proline uptake in C. albicans studied by Jayakumar et al (1979) showed that the K , for preincubated or proline-grown cells was similar to that of normal cells.…”

Section: N D a B R O W A A N D D H H O W A R Dmentioning

confidence: 99%

Proline Uptake in Candida albicans

Dabrowa¹,

Howard²

1981

Microbiology

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391~ L-Proline entered both mycelial and yeast cells of Candida albicans by an active transport system of high specificity at low ((0.1 mM) external concentrations of substrate. The apparent K, value of this system was 0-1 mM for both types of cells, while the Vvalue was 4 nmol min-' (mg dry wt)-' for mycelial cells and 1-4 nmol min-l (mg dry wt)-' for yeast cells. At L-proline concentrations greater than 0-1 mM, the amino acid appeared to enter both morphological forms by diffusion as well as active transport. As saturation was approached diffusion became increasingly important. The higher uptake rate of mycelial cells seemed not to be the result of an inducible system. The optimal pH and temperature for transport of L-proline were 7-0 and 37 O C , respectively. Sodium azide and the proline analogues sarcosine and L-azetidine-Zcarboxylic acid inhibited L-proline uptake, while ~-thiazolidine-4-carboxylic acid was less effective. The active transport system was highly specific for L-proline since neither ammonium ions, which inhibit the general amino acid transport system of fungi, nor 16 different amino acids interfered substantially with uptake.

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“…Excepting some nucleic acids (Brock, 1970), large colloids and peptides are first reduced to simple peptides or amino acids by exopeptidases activated by Mg, Mn or Fe, before crossing the membrane (Doelle, 1969). Substrate uptake (utilization) then proceeds by active transport (Kay and Gronlund, 1969) depending on substrate lability, i.e., how little energy need be expended by the cell to transform it into a product suitable for necessary syntheses or metabolic function. Utilization of nitrogen sources would then proceed: colloids < peptides < amine > NH4 > NO3 (Doelle, 1969;Brock, 1970).…”

Section: Don Productionmentioning

confidence: 99%

Interactions of dissolved and particulate nitrogen in lake metabolism

Manny¹

1971

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Weekly measurements of dissolved ammonia, nitrite, and nitrate for 22 months and organic nitrogen for 12 months were made in two hardwater lakes and seasonally in four other lakes representing a chemical and trophic spectrum in glaciated Michigan. Seston at 1 m depth was fractionated weekly for 14 months using 10 um Nitex netting in two lakes representing extremes of hardwater productivity.Net and nanno fractions were analyzed by micro-Kjeldahl digestion for organic nitrogen. Seasonal changes in all nitrogenous parameters were subsequently related to changes in fifteen other chemical and biological parameters assayed simultaneousl y.More than half of total sestonic pigments, algal cell volume, and organic nitrogen were present all year in the nannosestonic fraction in the unproductive and productive lake. These data supported the hypothesis that nannophytoplankton contain more nitrogen per unit cell volume than netphytoplankton. DISTRIBUT ION OF THIS DOCUMEN T IS UNLIMITEDfl DISCLAIMER This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency Thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. DISCLAIMERPortions of this document may be illegible in electronic image products. Images are produced from the best available original document.

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Proline transport by Pseudomonas aeruginosa

Cited by 37 publications

References 19 publications

The AlkB Monooxygenase of Pseudomonas oleovorans

The AlkB Monooxygenase of Pseudomonas oleovorans

Proline Uptake in Candida albicans

Interactions of dissolved and particulate nitrogen in lake metabolism

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