“…When bloodstream forms reach high density, either in culture or in a mammal, they have a quorum sensing response (Rojas et al, ) and transform to nondividing stumpy form trypomastigotes. Upon uptake of the stumpy forms by Tsetse, or transfer to culture at 27°C in the presence of cis‐aconitate, stumpy forms develop into growing procyclic trypomastigotes, which rely mainly on mitochondrial pathways of ATP generation (Bringaud, Riviere, & Coustou, ; Mantilla et al, ). A protein called PAD1 (protein associated with differentiation), which is expressed by stumpy forms, is implicated in cis‐ aconitate sensing (Dean, Marchetti, Kirk, & Matthews, ).…”