Proline is increased in allergic asthma and promotes airway remodeling

Xu, Tingting; Wu, Zhenzhen; Yuan, Qing; Zhang, Xijie; Liu, Yanan; Wu, Chaojie; Song, Ming; Wu, Jingjing; Jiang, Jingxian; Wang, Zhengxia; Chen, Zhongqi; Zhang, Mingshun; Huang, Mao; Ji, Ningfei

doi:10.1172/jci.insight.167395

Cited by 8 publications

(2 citation statements)

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“…Proline often becomes a rate-limiting substrate for collagen synthesis and is important in airway inflammation and remodeling. 39 It is possible to assess enzyme activity by examining the ratio of the coupled enzymatic substrate and product. In asthmatic mice, the ornithine/arginine ratio increased (Figure S10A), indicating elevated ARG activity.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Targeted Quantification of Glutathione/Arginine Redox Metabolism Based on a Novel Paired Mass Spectrometry Probe Approach for the Functional Assessment of Redox Status

Zhong,

Ling,

Yao

et al. 2024

Anal. Chem.

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Glutathione (GSH) redox control and arginine metabolism are critical in regulating the physiological response to injury and oxidative stress. Quantification assessment of the GSH/arginine redox metabolism supports monitoring metabolic pathway shifts during pathological processes and their linkages to redox regulation. However, assessing the redox status of organisms with complex matrices is challenging, and single redox molecule analysis may not be accurate for interrogating the redox status in cells and in vivo. Herein, guided by a paired derivatization strategy, we present a new ultraperformance liquid chromatography tandem mass spectrometry (UPLC−MS/MS)-based approach for the functional assessment of biological redox status. Two structurally analogous probes, 6-aminoquinolyl-Nhydroxysuccinimidyl carbamate (AQC) and newly synthesized 2-methyl-6aminoquinolyl-N-hydroxysuccinimidyl carbamate (MeAQC), were set for paired derivatization. The developed approach was successfully applied to LPS-stimulated RAW 264.7 cells and HDM-induced asthma mice to obtain quantitative information on GSH/arginine redox metabolism. The results suggest that the redox status was remarkably altered upon LPS and HDM stimulation. We expect that this approach will be of good use in a clinical biomarker assay and potential drug screening associated with redox metabolism, oxidative damage, and redox signaling.

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Section: ■ Results and Discussionmentioning

confidence: 99%

“…Proline levels were also increased in asthmatic mice (Figure F). Proline often becomes a rate-limiting substrate for collagen synthesis and is important in airway inflammation and remodeling . It is possible to assess enzyme activity by examining the ratio of the coupled enzymatic substrate and product.…”

Section: Resultsmentioning

confidence: 99%