Proline accumulation and Δ1-pyrroline-5-carboxylate synthetase gene properties in three rice cultivars differing in salinity and drought tolerance

Hien, Do Thu; Jacobs, M.; Angenon, Geert; Hermans, Christian; Thu, Tran Thanh; Sơn, Lê Văn; Roosens, Nancy

doi:10.1016/s0168-9452(03)00301-7

Cited by 114 publications

(60 citation statements)

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“…Growth characters, including SH, RL, LA, FW and DW, of rice grown under iso-osmotic water deficit and salt stress were reduced, related to the osmotic pressure in the culture media. This was especially the case under salt-induced osmotic stress and is similar to previous reports on the reduction of growth in rice exposed to iso-osmotic stress (Bahaji et al, 2002;Hien et al, 2003;Ahmad et al, 2007). In addition, fresh weight and dry weight in tolerant cultivars, including Basmati-370 and DR2, were maintained better than in the sensitive genotypes, Basmati-Kashmir and Cuom, when subjected to iso-osmotic stress derived from mannitol/PEG (polyethylene glycol) and NaCl salt (Hien et al, 2003;Ahmad et al, 2007).…”

Section: Discussionsupporting

confidence: 91%

Survival percentage, photosynthetic abilities and growth characters of two indica rice (Oryza sativa L. spp. indica) cultivars in response to iso-osmotic stress

Nishimura

Cha–um

Takagaki

et al. 1970

Span. j. agric. res.

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The aim of this study was to investigate pigment degradation, chlorophyll fluorescence diminution, photosynthetic ability and growth reduction in two rice cultivars, in response to either iso-osmotic salt stress or water-deficit stress. Seedlings of rice cultivars RD6 and KDML105 were photo-autotrophically grown in MS media and subsequently exposed to -0.23 (control), -0.40 or -0.67 MPa iso-osmotic NaCl (salt stress) or mannitol (water-deficit stress) for 14 days. The survival percentage of the two rice cultivars reduced dramatically when subjected to -0.67 MPa NaCl treatment. Chlorophyll a (Chl a ), chlorophyll b (Chl b ), total carotenoids (C x+c ), maximum quantum yield of PSII (F v /F m ) and photon yield of PSII (Φ PSII ) in the stressed seedlings were significantly lower when compared to seedlings in the control group (without mannitol or NaCl), leading to low net-photosynthetic rate (P n ) and growth reduction. In addition, the growth characters of plantlets in the salt stress conditions were more sharply reduced, and the physiological changes greater than those in water-deficit stress conditions. On the other hand, non-photochemical quenching in the leaves of stressed plantlets increased significantly, especially in response to iso-osmotic salt stress. In the present study, overall growth performance and physiological characters of KDML105 grown under iso-osmotic stress were better than those of RD6.Additional key words: net-photosynthetic rate, photosynthetic pigments, salt stress, water deficit. ResumenPorcentaje de supervivencia, capacidad fotosintética y crecimiento de dos cultivares de arroz tipo índica (Oryza sativa L. spp. indica) en respuesta al estrés iso-osmótico El objetivo de este estudio fue investigar la degradación de pigmentos, la disminución de la fluorescencia de la clorofila, la capacidad fotosintética y la reducción en el crecimiento de dos cultivares de arroz, en respuesta al estrés salino iso-osmótico o hídrico. Se cultivaron de forma foto-autotrófica en medio MS plántulas de los cultivares RD6 y KDML105 y posteriormente se expusieron durante 14 días a NaCl (estrés salino) o manitol (estrés hídrico) iso-osmótico -0,23 (control), -0,40 ó -0,67 MPa. El porcentaje de supervivencia de los dos cultivares de arroz se redujo drásticamente con el tratamiento de NaCl 0,67 MPa. La clorofila a (Chl a ), clorofila b (Chl b ), carotenoides totales (C x+c ), rendimiento cuántico máximo del PSII (F v /F m ) y el rendimiento de fotones del PSII (Φ PSII ) fueron significativamente menores en las plántulas estresadas respecto a las plántulas del grupo control (sin manitol o NaCl), lo que les llevó a una baja tasa de fotosíntesis neta (P n ) y a una reducción del crecimiento. Además, el crecimiento de las plántulas bajo estrés salino se redujo drásticamente y los cambios fisiológicos fueron mayores que bajo estrés hídrico. Por otra parte, el amortiguamiento no fotoquímico (NPQ) en las hojas de las plántulas estresadas aumentó considerablemente, especialmente en respuesta al estrés salino iso-o...

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Section: Discussionsupporting

confidence: 91%

Survival percentage, photosynthetic abilities and growth characters of two indica rice (Oryza sativa L. spp. indica) cultivars in response to iso-osmotic stress

Nishimura

Cha–um

Takagaki

et al. 1970

Span. j. agric. res.

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“…El aumento de la concentración de prolina en el tejido apical radical debido a la reducción de agua ha sido observado en especies cultivadas, como Phaseolus vulgaris, Zea mays (Sánchez-Urdaneta et al, 2003, 2005 y Oryza sativa (Hien et al, 2003), pero también en plántulas de Agave salmiana (Peña-Valdivia y Sánchez-Urdaneta, 2009). Es probable que el aumento de prolina registrado en algunas especies de Agave indique también una función de regulación osmótica (Hare y Cress, 1997), la cual se manifestó en mayor crecimiento de la raíz respecto al vástago en A. angustifolia subsp.…”

Section: Discussionunclassified

Respuestas bioquímico-fisiológicas de especies de Agave a la restricción de humedad

2014

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4Autor para la correspondencia: hugo.ramirez@uaslp.mxResumen: En México se encuentra la mayor riqueza de especies de Agave, distribuidas en ambientes diversos. Para contribuir con el conocimiento en torno a las formas de tolerar las restricciones de humedad se evaluó el efecto de dos potenciales de agua (Ψ) en el sustrato (-0.7 y -3.5 MPa) durante 14 meses, en invernadero, sobre el crecimiento, la distribución de biomasa, el grosor foliar y el contenido de prolina en dos especies del subgénero Littaea (A. lechuguilla y A. striata) y seis del subgénero Agave (A. americana var. marginata, A. angustifolia subsp. tequilana, A. asperrima, A. cupreata, A. duranguesis y A. salmiana), reconocidas por su importancia económica y ecológica. La menor disponibilidad de humedad inhibió la acumulación de biomasa en las ocho especies; no obstante, el crecimiento (número de hojas y cobertura de planta) pudo mantenerse en aquellas más adaptadas a la aridez o con límites de tolerancia ambiental más amplios como A. angustifolia subsp. tequilana, A. duranguensis, A. lechuguilla y A. salmiana. El Ψ limitante promovió mayor asignación de biomasa a la raíz a expensas de la de las hojas en algunas especies y la duplicación de la prolina radical en las especies del subgénero Agave. La modifi cación de la distribución de la biomasa y el incremento del contenido de prolina, como formas de tolerar la restricción de humedad, sólo se presentaron en algunas de las especies estudiadas. En contraste, el mantenimiento del grosor de la hoja (indicador de turgencia) fue una respuesta general al Ψ limitante. Las especies de Agave presentan respuestas fi siológicas y bioquímicas variadas, que refl ejan su amplia tolerancia a condiciones ambientales adversas y explican la distribución ecológica amplia del género. Palabras clave: crecimiento, distribución de biomasa, potencial de agua, prolina, suculencia.Abstract: Mexico has the greatest richness of Agave species, distributed in diverse environments. In order to contribute to the knowledge on ways to tolerate moisture constraints, the effect of two water potential (Ψ) in the substrate (-0.7 and -3.5 MPa) on growth, biomass allocation, leaf thickness and proline content in two species of the subgenus Littaea (A. lechuguilla and A. striata), and six of the subgenus Agave (A. americana var. marginata, A. angustifolia subsp. tequilana, A. asperrima, A. cupreata, A. duranguesis and A. salmiana), recognized for its economic and ecological importance, was evaluated during 14 months and under greenhouse conditions. Low moisture inhibited biomass accumulation in the eighth species; despite this, growth (number of leaves and plant coverage) kept in those more adapted to aridity or with broader environmental tolerant limits as A. angustifolia subsp. tequilana, A. duranguensis, A. lechuguilla, and A. salmiana. The limiting Ψ promoted greater biomass allocation to roots at expense of leaves in some species, and duplication of radical proline in species of the subgenus Agave. The modifi cation of biomass allocati...

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“…For example, proline content in PT1 and IR20 (water deficit sensitive) peaked in plants subjected to severe water deficit (7 % SWC) and was higher than in the tolerant cultivars (KDML105 and NSG19) (Cha-um et al, 2010). Similarly, proline in the leaf tissues of drought sensitive, CR203 (3.12 times) is accumulated higher than in drought tolerant cultivars, DR2 (1.41 times) and Cuom (2.06 times), when plants are subjected to water deficit stress (Hien et al, 2003). In contrast, P5CR and P5CS genes in proline biosynthesis in drought tolerant (Ca/H680) are up-regulated resulting in enriched proline contents greater than those in drought sensitive (Ca/H148) when exposed to drought stress (Parida et al, 2008).…”

Section: Resultsmentioning

confidence: 94%

Water-deficit tolerant classification in mutant lines of indica rice

Cha–um

Yooyongwech

Supaibulwatana

2012

Sci. agric. (Piracicaba, Braz.)

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Water shortage is a major abiotic stress for crop production worldwide, limiting the productivity of crop species, especially in dry-land agricultural areas. this investigation aimed to classify the water-deficit tolerance in mutant rice (Oryza sativa L. spp. indica) genotypes during the reproductive stage. Proline content in the flag leaf of mutant lines increased when plants were subjected to water deficit. relative water content (rWC) in the flag leaf of different mutant lines dropped in relation to water deficit stress. A decrease rWC was positively related to chlorophyll a degradation. Chlorophyll a , chlorophyll b , total chlorophyll , total carotenoids , maximum quantum yield of PSII , stomatal conductance , transpiration rate and water use efficiency in mutant lines grown under water deficit conditions declined in comparison to the well-watered, leading to a reduction in net-photosynthetic rate. In addition, when exposed to water deficit, panicle traits, including panicle length and fertile grains were dropped. the biochemical and physiological data were subjected to classify the water deficit tolerance. NSG19 (positive control) and DD14 were identified as water deficit tolerant, and AA11, AA12, AA16, bb13, bb16, CC12, CC15, EE12, FF15, FF17, G11 and Ir20 (negative control) as water deficit sensitive, using Ward's method.

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Proline accumulation and Δ1-pyrroline-5-carboxylate synthetase gene properties in three rice cultivars differing in salinity and drought tolerance

Cited by 114 publications

References 39 publications

Survival percentage, photosynthetic abilities and growth characters of two indica rice (Oryza sativa L. spp. indica) cultivars in response to iso-osmotic stress

Survival percentage, photosynthetic abilities and growth characters of two indica rice (Oryza sativa L. spp. indica) cultivars in response to iso-osmotic stress

Respuestas bioquímico-fisiológicas de especies de Agave a la restricción de humedad

Water-deficit tolerant classification in mutant lines of indica rice

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