Proline accumulation and the expression of Δ&lt;sup&gt;1&lt;/sup&gt;-pyrroline-5-carboxylate synthetase in two safflower cultivars

Thippeswamy, M.; Chandraobulreddy, P.; Sinilal, B.; Kumar, Manoj; Sudhakar, Chinta

doi:10.1007/s10535-010-0070-7

Cited by 22 publications

(7 citation statements)

References 36 publications

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“…In plant cell it has been reported that methionine as a fundamental metabolite through its first metabolite S-adenosylmethionine synthetase [39]. In contrast to our results, Thippeswamy et al [20] reported the water stress tolerance mechanism in safflower was controlled by pro which was due to increased activity of P5CS. In safflower, the range of met is 1.4-1.5%, pro 8.5-9.5% and the range of pro is higher than the met.…”

Section: Overall Amino Acid Distribution In All the Ests Librariescontrasting

confidence: 99%

“…The unigene sets of all the libraries were searched for SSRs using SSRLocator [18][19][20][21] and the abundance of amino acids predicted from the trinucleotide repeats were compared among all the libraries. SSR locator was used to search SSRs (motifs ranging from 1 to 10 nucleotides) in order to evaluate the pattern of EST-SSR distribution among all libraries.…”

Section: Identification and Comparison Of Est-ssr And Amino Acid Distmentioning

confidence: 99%

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In silico Mining of EST-SSRs from the Drought Tolerant ESTs in Safflower

Sudhakar¹,

Thippeswamy²,

Sivakumar³

et al. 2019

J Proteomics Bioinform

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Drought stress is a major abiotic factor causing yield loss in safflower and limited studies have been carried out to dissect the molecular mechanism at EST-SSR level. In this study, a possible relationship between simple sequence repeats (SSRs) distribution and drought stress was studied using three EST libraries of safflower, Ct-D-EST, Ct-NEST and Co-EST. The Ct-D-EST was generated from drought tolerant safflower cultivar A-1; Ct-NEST and Co-EST were the EST datasets of safflower and its wild progenitor C. oxycanthus, respectively. In total, 156 (45%), 1194 (5%) and 1550 (9%) EST-SSRs were mined from Ct-D-EST, Ct-NEST and Co-EST libraries, respectively. Comparison of EST-SSRs from Ct-D-EST with that of SSRs from other two libraries showed reasonable differences for each class of repeats. Large variations were observed for dinucleotide repeats in all the libraries. In drought EST-SSRs, only one kind of amino acid was produced by repeat (ATG) 14 which encodes met indicating the loci and repeat observed to be 100%. Since three ESTs with SSRs from Ct-D-EST, annotated to putative candidate genes, S-adenosylmethionine synthetase, one-helix protein and myo-inositol 1-phosphate synthetase, did not express with SSRs in the Ct-NEST , these can be considered as potential candidate genes for drought tolerance. A trinuceotinde SSR, encoding met, was also found in the EST annotated to putative s-adenosylmethionine synthetase, hence, both can be considered as indices for drought tolerance. The genomic resources and the information generated in this study may be useful for the safflower breeders particularly for the development of drought tolerant cultivar.

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Section: Overall Amino Acid Distribution In All the Ests Librariescontrasting

confidence: 99%

Section: Identification and Comparison Of Est-ssr And Amino Acid Distmentioning

confidence: 99%

In silico Mining of EST-SSRs from the Drought Tolerant ESTs in Safflower

Sudhakar¹,

Thippeswamy²,

Sivakumar³

et al. 2019

J Proteomics Bioinform

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“…However, the two distinct committed steps are regarded as rate limiting and two enzymes are γ-GK and γ-GPR. These two enzymes are operative in a sequential manner for biosynthesis of proline and have been evident to relate the redox balance under osmotic stress (Thippeswamy et al 2010). Recently, the effects of cadmium and zinc stress on these enzyme activities have been studied and revealed the regulatory property of proline in homeostasis of ions (Wang et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Effects of putrescine on oxidative stress induced by hydrogen peroxide in Salvinia natans L.

Mandal

Ghosh

Dey

et al. 2014

Journal of Plant Interactions

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Salvinia natans L. response to hydrogen peroxide (H 2 O 2 ) induced oxidative stress through physiological activities was evaluated. The plants were incubated with varying concentrations (0, 50, 100 µM) of H 2 O 2 and 100 µM of H 2 O 2 supplemented with 1 mM putrescine (Put) in hydroponic culture. This is observed with the decline in proline content and its biosynthetic enzymes viz. γ-glutamyl kinase and γ-glutamyl phosphate reductase activity. Protein carbamylated derivative by protein oxidation was another trait for oxidative damages by H 2 O 2 . The antioxidative enzymes like guaiacol peroxidase (GPX), glutathione reductase (GR), and catalase (CAT) recorded to express through in-gel staining with the H 2 O 2 exposure. On nuclear level, plants were sensitive to H 2 O 2 where the DNA disintegration was studied with comet assay and maximum comet tail observed at 100 µM H 2 O 2 treatment. Application of Put reduced the generation of protein oxidation and comet tail length as well as moderated the enzyme activity as revealed through in-gel staining.

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“…There are two proline synthetic pathways in higher plants (Charest and Phan 1990). One is the conversion of glutamate via the action of pyrroline-5-carboxylate synthetase (P5CS) (Voetberg and Sharp ⎯⎯⎯⎯ 1991, Turchetto-Zolet et al 2009, Thippeswamy et al 2010, while the other is the conversion of ornithine (Canas et al 2008, Funck et al 2008) via ornithine aminotransferase (OAT). Conversely, proline can be catabolized to glutamate via the action of proline dehydrogenase (PDH; Kiyosue et al 1996, Peng et al 1996, Verbruggen et al 1996.…”

Section: Introductionmentioning

confidence: 99%

The effect of water deficit and excess copper on proline metabolism in Nicotiana benthamiana

Ku¹,

Tan²,

Su³

et al. 2012

Biologia plant.

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Fluctuation in proline content is a widespread phenomenon among plants in response to heavy metal stress. To distinguish between the participation of water deficit and copper on changes in proline metabolism, potted plants and floating leaf discs of tobacco were subjected to CuSO4 treatments. The application of copper increased the proline content in the leaves concomitantly with decreased leaf relative water content and increased abscisic acid (ABA) content in the potted plant. Excess copper increased the expression of two proline synthesis genes, pyrroline-5-carboxylate synthetase (P5CS) and ornithine aminotransferase (OAT) and suppressed proline catabolism gene, proline dehydrogenase (PDH). However, in the experiment with tobacco leaf discs floating on CuSO4 solutions, the excess copper decreased proline content and suppressed the expression of the P5CS, OAT and PDH genes. Therefore, proline accumulation in the potted tobacco plants treated with excess Cu treatment might not be the consequence of the increased copper content in tobacco leaves but rather by the accompanied decrease in water content and/or increased ABA content

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Proline accumulation and the expression of Δ<sup>1</sup>-pyrroline-5-carboxylate synthetase in two safflower cultivars

Cited by 22 publications

References 36 publications

In silico Mining of EST-SSRs from the Drought Tolerant ESTs in Safflower

In silico Mining of EST-SSRs from the Drought Tolerant ESTs in Safflower

Effects of putrescine on oxidative stress induced by hydrogen peroxide in Salvinia natans L.

The effect of water deficit and excess copper on proline metabolism in Nicotiana benthamiana

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