Proline accumulation and glutathione reductase activity induced by drought-tolerant rhizobacteria as potential mechanisms to alleviate drought stress in Guinea grass

Dry-Caribbean Bacillus spp. Strains Ameliorate Drought Stress in Maize by a Strain-Specific Antioxidant Response Modulation

“…Five strains of Bacillus spp. previously isolated from the Dry-Caribbean region of Colombia [ 15 ] were screened for their ability to ameliorate drought stress in maize plants in this study. The 16S rRNA gene was amplified by PCR and sequenced as previously described [ 15 ].…”

“…previously isolated from the Dry-Caribbean region of Colombia [ 15 ] were screened for their ability to ameliorate drought stress in maize plants in this study. The 16S rRNA gene was amplified by PCR and sequenced as previously described [ 15 ]. The almost complete 16S rRNA genes of strains XT13 (1344 bp), XT14 (1266 bp), XT38 (905 bp), and XT110 (1328 bp) were then aligned with those of the type material strains of the genera belonging to the order Bacillales obtained from the NCBI Reference RNA Sequences database.…”

“…The almost complete 16S rRNA genes of strains XT13 (1344 bp), XT14 (1266 bp), XT38 (905 bp), and XT110 (1328 bp) were then aligned with those of the type material strains of the genera belonging to the order Bacillales obtained from the NCBI Reference RNA Sequences database. The strains sequences of 16S rRNA were deposited previously in NCBI by [ 15 ]. The accession numbers of the strains were XT13—MH014814; XT14—MH014816; XT17—MH014818; XT38—MH014817; and XT110—MH014815.…”

“…A sample of 100 mg of the ground tissue was mixed with 2 mL of 0.2 M phosphate buffer (pH 7.8) containing 0.1 mM of EDTA in 2.5-mL microtubes and softly homogenized with a vortex. The homogenate was centrifuged at 15,000× g for 20 min at 4 °C (Eppendorf 5415C Centrifuge, Hamburg, Germany), and the supernatant was used to establish the enzymatic activities [ 15 , 32 ].…”

“…In a previous study, a Bacillus spp. consortium modulated Megathyrsus maximus (Guinea grass) antioxidant response leading to drought stress amelioration through biomass production increase [ 15 ]. These strains may lead to the development of biotechnologies to increase crop production in arid and semiarid regions due to their ability to endure harsh environments and mitigate plant drought stress.…”