Proliferative response of peripheral blood lymphocytes to mitogens in the owl monkey <i>Aotus nancymae</i>

Pinzón-Charry, Alberto; Vernot, Jean-Paul; Rodríguez, Ricaurte; Patarroyo, Manuel E.

doi:10.1034/j.1600-0684.2003.00004.x

Cited by 8 publications

(6 citation statements)

References 41 publications

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“…As the animals were randomly chosen from a commercial farm, even if they were from the same breed, the differences in their genetic backgrounds could have contributed to the observed variations. Treatment with a combination of PMA and ionomycin activates lymphocytes, as well as other leukocytes, by mimicking the intracellular signals that occur during natural responses to antigens (20,29). A number of studies have investigated the effect of PMA-Iono on the proliferation and expression of cytokines in human and animal PBMCs.…”

Section: Discussionmentioning

confidence: 99%

Development of a Macroarray To Specifically Analyze Immunological Gene Expression in Swine

Ledger

Pinton

Bourges

et al. 2004

Clin Vaccine Immunol

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DNA arrays are useful tools for simultaneously studying the expressions of a large number of genes. Herein, we describe the construction and the optimization of conditions for a low-density DNA macroarray specific for the porcine immune system. This specific DNA macroarray contains 63 gene products, including 20 cytokines, 11 chemokines, and 12 immunologically relevant receptors. It was constructed by designing gene-specific oligonucleotide primers from porcine sequences available in the EMBL or TIGR expressed sequence tag data bank and using primers from conserved regions of aligned sequences from other species for sequences unavailable for swine. Amplicons produced by reverse transcription-PCR were cloned, sequenced, and spotted onto nylon filters. A trial DNA array was first produced to optimize the intensity, specificity, and variability of signals from amplicons amplified with either gene-specific or universal primers. The DNA macroarray was then validated by comparing the gene expression profile of nonstimulated peripheral blood mononuclear cells (PBMCs) to that of phorbol 12-myristate 13-acetate and ionomycin (PMA-Iono)-stimulated PBMCs from three different animals over a 48-h time period. As already described for more conventional techniques, we showed that certain genes, such as those for CD40, gamma interferon, interleukin 2 (IL-2), the IL-2 receptor, and tumor necrosis factor alpha, were upregulated in PMA-Iono-stimulated PBMCs. A detailed analysis also indicated a downregulation of several genes which are expressed mainly by macrophages (IL-1, IL-8, AMCF-1, natural-resistance-associated macrophage protein, neutrophil chemotactic protein, DAP-12, and monocyte chemoattractant protein) in samples stimulated for 24 h with PMA-Iono compared to their levels of expression in control samples. These results indicate that the DNA macroarray that we constructed can be a useful tool for simultaneously monitoring the mRNA expression of immunologically relevant genes in different porcine samples.

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Section: Discussionmentioning

confidence: 99%

Development of a Macroarray To Specifically Analyze Immunological Gene Expression in Swine

Ledger

Pinton

Bourges

et al. 2004

Clin Vaccine Immunol

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“…The increased proliferation was dependent on the cargo sequence since proliferation of PBMC treated with hTip alone or the chimeric CSKH scrambled peptide (hTip-CSKHsc) was as low as that of control cells without peptide (not shown). Some variability in Aotus response to hTip-CSKH compared to humans was observed, but this is probably due to what we have previously described for this experimental model [ 43 ]. The hTip-CSKH effect is then similar to PHA-P, that is, inducing proliferation by its own, although to a lesser extent.…”

Section: Resultsmentioning

confidence: 80%

“…The Aotus primate experimental model has proved to be useful for basic immunology and vaccination studies. The establishment of similarities is essential for physiological studies involving immune modulation, although some differences in T-cell response have been found [ 43 ]. Previously, we found that Aotus Lck was more related to Saimiri sciureus than to human Lck.…”

Section: Resultsmentioning

confidence: 99%

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Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip fromHerpesvirus saimiri

Vernot

Perdomo-Arciniegas

Perez-Quintero

et al. 2015

Journal of Immunology Research

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The Lck interacting protein Tip of Herpesvirus saimiri is responsible for T-cell transformation both in vitro and in vivo. Here we designed the chimeric peptide hTip-CSKH, comprising the Lck specific interacting motif CSKH of Tip and its hydrophobic transmembrane sequence (hTip), the latter as a vector targeting lipid rafts. We found that hTip-CSKH can induce a fivefold increase in proliferation of human and Aotus sp. T-cells. Costimulation with PMA did not enhance this proliferation rate, suggesting that hTip-CSKH is sufficient and independent of further PKC stimulation. We also found that human Lck phosphorylation was increased earlier after stimulation when T-cells were incubated previously with hTip-CSKH, supporting a strong signalling and proliferative effect of the chimeric peptide. Additionally, Lck downstream signalling was evident with hTip-CSKH but not with control peptides. Importantly, hTip-CSKH could be identified in heavy lipid rafts membrane fractions, a compartment where important T-cell signalling molecules (LAT, Ras, and Lck) are present during T-cell activation. Interestingly, hTip-CSKH was inhibitory to Jurkat cells, in total agreement with the different signalling pathways and activation requirements of this leukemic cell line. These results provide the basis for the development of new compounds capable of modulating therapeutic targets present in lipid rafts.

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“…Recientemente hemos caracterizado algunas moléculas de Aotus sp. que son importantes en la transducción de señales y hemos realizado estudios funcionales con células tendientes a comparar los respuestas inmunes (22)(23)(24).…”

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Caracterización de la cadena CD3 épsilon en el primate del nuevo mundo Aotus nancymaae

Castillo

Vernot

2008

biomedica

Self Cite

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Introducción. El complejo asociado al receptor de las células T está constituido por las moléculas CD3 (δ, γ, ε) y las cadenas ζ, todas proteínas transmembrana esenciales para la transducción de señales durante la activación del linfocito y la respuesta inmune, así como durante el desarrollo de los timocitos. Objetivo. En este trabajo se buscaba determinar la estructura primaria de la cadena CD3ε, en el mono del nuevo mundo, Aotus nancymaae. Materiales y métodos. A partir del ARN total obtenido de células mononucleares de sangre periférica, se amplificó la molécula de CD3ε, luego se clonó en un vector y, finalmente, se secuenció. Resultados. Se presenta la secuencia deducida de aminoácidos de la cadena CD3ε de A. nancymaae. Se estableció una identidad de 84% y de 76% en la secuencia nucleotídica y la de aminoácidos, respectivamente, con su contraparte humana. La molécula de Aotus muestra bastante variabilidad en la región extracelular y bastante conservación en la intracelular. Están conservadas subregiones del ectodominio que son importantes para el plegamiento de la molécula, así como para la asociación con las otras cadenas del complejo. Conclusiones. La estructura primaria determinada aquí sugiere que la proteína de Aotus tiene una funcionalidad similar y que los pasos iniciales de activación de la célula T se suceden como en los humanos; por otra parte, la gran variabilidad encontrada en el ectodominio, permite explicar por qué algunos anticuerpos monoclonales dirigidos contra el complejo CD3 de humano no reconocen estas estructuras en el Aotus.Palabras clave: sistema inmune, receptores del antígeno de célula T, antígenos CD3, proteínas adaptadoras transductoras de señales.

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Proliferative response of peripheral blood lymphocytes to mitogens in the owl monkey Aotus nancymae

Cited by 8 publications

References 41 publications

Development of a Macroarray To Specifically Analyze Immunological Gene Expression in Swine

Development of a Macroarray To Specifically Analyze Immunological Gene Expression in Swine

Modulating p56Lck in T-Cells by a Chimeric Peptide Comprising Two Functionally Different Motifs of Tip fromHerpesvirus saimiri

Caracterización de la cadena CD3 épsilon en el primate del nuevo mundo Aotus nancymaae

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