Proliferative response of cultured human tenon's capsule fibroblasts to platelet-derived growth factor isoforms

Knorr, M.; Völker, Michael; Denk, Peter Oliver; Wunderlich, Kerstin; Thiel, Holger

doi:10.1007/bf00946945

Cited by 17 publications

(18 citation statements)

References 26 publications

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“…Several studies reported that these factors are potent stimulators of HTF proliferation in vitro (Cunliffe et al, 1995;Denk et al, 2003;Knorr et al, 1997). Furthermore, inhibition of some of these molecules diminished growth of cultured human TF (Salas-Prato et al, 1996) and improved glaucoma filtration surgery outcome in an experimental glaucoma model (Turgut et al, 2014).…”

Section: Other Growth Factors and Cytokinesmentioning

confidence: 97%

Modulation of wound healing in glaucoma surgery

Velde¹,

Bergen²,

Vandewalle³

et al. 2015

Progress in Brain Research

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Section: Other Growth Factors and Cytokinesmentioning

confidence: 97%

Modulation of wound healing in glaucoma surgery

Velde¹,

Bergen²,

Vandewalle³

et al. 2015

Progress in Brain Research

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“…Different studies have shown that these factors can stimulate proliferation of Tenon fibroblasts in vitro 81,82. Moreover, inhibition of some of these factors reduced growth of Tenon fibroblasts83 and improved surgical outcome in a rabbit model of glaucoma 84,85.…”

Section: State Of the Art In Modulation Of Wound Healingmentioning

confidence: 99%

Improving patient outcomes following glaucoma surgery: state of the art and future perspectives

Bergen¹,

Velde²,

Vandewalle³

et al. 2014

OPTH

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Of all the treatments currently used to lower intraocular pressure in glaucoma patients, filtration surgery is known to be the most effective. However, in a significant percentage of cases, the constructed channel closes due to excessive scar formation, resulting in surgical failure. The process of postoperative wound healing is characterized by the coagulative and inflammatory phase, followed by the proliferative and repair phase, and finally the remodeling phase. Perioperative antimitotic agents, such as mitomycin C and 5-fluorouracil, are known to modulate the process of wound healing and to improve surgical outcome, but they carry a risk of vision-threatening complications. New alternative strategies to prevent filtration failure, such as inhibition of transforming growth factor-β, vascular endothelial growth factor, and placental growth factor, have shown promising results in the improvement of surgical success. However, it remains necessary to broaden the therapeutic approach by focusing on combined therapies and on extended drug delivery.

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“…Studies by Denk et al strongly support the hypothesis that PDGF isoforms are major stimulators of proliferation of Tenon’s capsule fibroblasts after GFS, while TGF-β isoforms are essential for the transformation of Tenon’s capsule fibroblasts into myofibroblasts 25,44. In another study, Knorr et al demonstrated that PDGF-AB and -BB isoforms are potent stimulators of the proliferation of human Tenon’s capsule fibroblasts, suggesting that they play an important role in the wound healing response after GFS 26. PDGF is normally present in trace amounts in the aqueous humor and increases the proliferative response of fibroblasts to TGF-β 44.…”

Section: Discussionmentioning

confidence: 76%

“…TGF-β is the most potent stimulator of fibroblast activity 5–7,22,23. Additionally, after conjunctival and scleral surgery, PDGF is released from platelets, which promotes cell migration into the wound site and increases fibroblast proliferation 6,7,22–26. Thus, the inhibition of fibroblast function after GFS is an important factor in the wound healing process and for the outcome of GFS.…”

Section: Discussionmentioning

confidence: 99%

Topical infliximab for the suppression of wound healing following experimental glaucoma filtration surgery

Turgut¹,

Eren²,

Akın³

et al. 2014

DDDT

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BackgroundThe purpose of this work was to look into the effects of infliximab on wound healing in experimental glaucoma filtration surgery and to compare the antifibrotic effects of this agent to that of mitomycin-C (MMC).MethodsTwenty-eight male New Zealand White rabbits were randomly assigned to four groups, each including seven rabbits: control group, sham group, MMC group, and infliximab group. The rabbits in the control group were not operated on and did not receive any treatment. The rabbits in the sham group underwent trabeculectomy and had one drop of saline instilled four times a day for 14 days. The rabbits in the MMC treatment group underwent trabeculectomy, and a sponge soaked in 0.4 mg/mL MMC was applied intraoperatively to the scleral surgical site for three minutes. The rabbits in the infliximab treatment group underwent trabeculectomy and one drop of 10 mg/mL infliximab was instilled four times a day for 14 days after surgery. On day 14 of the experiment, the operated and control eyes were enucleated and histologically and immunohistochemically analyzed.ResultsThe mean fibroblast and mononuclear cell (MNC) numbers and the mean immunostaining intensities of transforming growth factor-β (TGF-β), fibroblast growth factor-β (FGF-β), and platelet-derived growth factor (PDGF) in the sham group were higher than those of the control group (P<0.01). The mean fibroblast and MNC numbers and the mean immunostaining intensities of TGF-β, FGF-β, and PDGF in the MMC and infliximab groups were statistically significantly lower than those of the sham group (P<0.01). The mean fibroblast and MNC numbers and the mean TGF-β, FGF-β, and PDGF immunostaining intensities of the MMC and infliximab groups were similar (P>0.05).ConclusionOur study suggests that topical infliximab effectively suppresses the subconjunctival wound healing response after experimental glaucoma filtration surgery, reducing the MNC and fibroblast numbers and immunostaining intensities of TGF-β, FGF-β, and PDGF.

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Proliferative response of cultured human tenon's capsule fibroblasts to platelet-derived growth factor isoforms

Abstract: Our results indicate that PDGF-AB and -BB isoforms are potent stimulators of proliferation of human Tenon's capsule fibroblasts, suggesting that PDGF-AB and -BB isoforms play an important role in the wound-healing response after glaucoma filtration surgery.

Cited by 17 publications

References 26 publications

Modulation of wound healing in glaucoma surgery

Modulation of wound healing in glaucoma surgery

Improving patient outcomes following glaucoma surgery: state of the art and future perspectives

Topical infliximab for the suppression of wound healing following experimental glaucoma filtration surgery

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