Proliferative Potentials of Bone Marrow and Blood Cells Studied by in vitro Uptake of H&lt;sup&gt;3&lt;/sup&gt;-Thymidine

Bond, V.P.; Fliedner, Theodor M.; Cronkite, Eugene P.; Rubini, Joseph R.; Brecher, George; Schork, P.

doi:10.1159/000205651

Cited by 142 publications

(35 citation statements)

References 11 publications

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“…Using liquid scintillation counting methods, Horwitz, Stastny, and Ziff suggested that increased mononuclear uptake of tritiated thymidine (Tdr-8H)1 occurred especially in the acute phase of an inflammatory disease and may reflect either a response to nonspecific tissue injury, or antigenic stimulation (2). Radioautographic studies were in agreement with other investigators who have shown that DNA-synthesizing cells are large mononuclear cells (3,4) which have been called "atypical lymphocytes" (5).…”

Section: Introductionsupporting

confidence: 90%

“…Many of these were morphologically similar to the "stress lymphocytes" of Frank and Dougherty (10), and as a group to the "atypical lymphocytes" of Wood and Frenkel (5). 35% of these large nonphagocytic mononuclears were DNAsynthesizing cells similar to those originally described by Bond and coworkers (3) and recently by Horwitz, Stastny, and Ziff (2). Tdr-2H-labeled large phagocytic cells (monocytes) or small lymphocytes were rare.…”

Section: Circulating Proliferating Macrophage Precursors In Inflammatsupporting

confidence: 64%

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The Development of Macrophages from Large Mononuclear Cells in the Blood of Patients with Inflammatory Disease

Horwitz¹

1972

J. Clin. Invest.

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Section: Introductionsupporting

confidence: 90%

Section: Circulating Proliferating Macrophage Precursors In Inflammatsupporting

confidence: 64%

The Development of Macrophages from Large Mononuclear Cells in the Blood of Patients with Inflammatory Disease

Horwitz¹

1972

J. Clin. Invest.

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“…At this time, activity in terms of thymidine uptake was 5-to 10-fold less, and mitoses, labeled or unlabeled were never found before the 32nd hr of culture. It was considered that this initial labeling activity did not represent a specific immune response since control cultures behaved in a similar manner, but rather, probably reflected either an adjustment of the blood leucocytes to the culture conditions, or the known low level of DNA synthesis in cells of normal peripheral blood (22). By the 44th hr after the mixed cell cultures were initiated (group C, 33 ~ hr) the levels of 3HTdR incorporation in them were significantly elevated over those in the control cultures, and the first labeled nuclei (0.4%) and mitotic figures appeared.…”

Section: Magnitude and Kinetics Of The Response-mentioning

confidence: 99%

Quantitative Studies on the Mixed Lymphocyte Interaction in Rats

Wilson

Blyth

Nowell̀

1968

The Journal of Experimental Medicine

248

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The proliferative interaction of cultured rat lymphocytes of immunogenetically disparate origin—the mixed lymphocyte interaction—was employed as an experimental model to examine the initial stages of the immune response mechanism. Using mixed cultures of cells derived from parental strain and F1 hybrid rats, in which only the parental lymphocytes respond, the following observations were made on the magnitude and kinetics of the reaction. After initiation of the cultures, there was a latent period of approximately 40 hours during which time no mitotic activity was detected. This inactive phase was followed by a period of proliferation in which previously nondividing cells entered the mitotic cycle for the first time. Activity in the cultures, as detected by incorporation of radioactive thymidine and measured by radioautography or scintillation spectrometry, increased exponentially with a doubling time (T2) of 9–10 hr. In this exponential proliferative phase, lasting approximately 100 hr, the dividing cells underwent a series of rapid sequential divisions with a generation time (Tc) of 8 hr, and few, if any, dropped out of the mitotic cycle. In addition to the cells which first entered mitosis at the beginning of the proliferative phase and then proceeded through multiple divisions, significant numbers of new, previously nondividing cells continued to enter the mitotic cycle during the entire exponential growth phase. The total number of these newly responsive, first division cells throughout the total culture period amounted to 1–3% of the original parental cell inoculum. This is a surprisingly large proportion of peripheral blood lymphocytes with demonstrable reactivity to a particular antigen system, if it is assumed that these first division cells in vitro are functionally related to the hypothetical antigen-sensitive cells which proliferate and differentiate into immunological effector cells. At present there is no entirely satisfactory explanation for this large number of reactive cells in the mixed lymphocyte interaction.

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“…This opinion has received support from many experimental observations including the lack of deoxyribonucleic acid (DNA) synthesis in these cells (1), their low rate of protein synthesis (2,3), and their long life span (4,5). On Received for publication 15 September 1967 and in revised form 15 November 1967. cellular (6) and humoral (7) immune reactions has been firmly established.…”

Section: Introductionmentioning

confidence: 99%

Characteristics of the RNA synthesized in vitro by the normal human small lymphocyte and the changes induced by phytohemagglutinin stimulation

Torelli¹,

Henry²,

Weissman³

1968

J. Clin. Invest.

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Proliferative Potentials of Bone Marrow and Blood Cells Studied by in vitro Uptake of H<sup>3</sup>-Thymidine

Cited by 142 publications

References 11 publications

The Development of Macrophages from Large Mononuclear Cells in the Blood of Patients with Inflammatory Disease

The Development of Macrophages from Large Mononuclear Cells in the Blood of Patients with Inflammatory Disease

Quantitative Studies on the Mixed Lymphocyte Interaction in Rats

Characteristics of the RNA synthesized in vitro by the normal human small lymphocyte and the changes induced by phytohemagglutinin stimulation

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