Proliferative opisthomastigote forms in Herpetomonas roitmani (Kinetoplastida: Trypanosomatidae)

Faria-e-Silva, Paulo M.; Soares, Maurílio J.; Souza, Wanderley de

doi:10.1007/s004360050082

Cited by 9 publications

(7 citation statements)

References 20 publications

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“…Another interesting aspect of our study is that during the protozoan cytokinesis, the bacterium and the kinetoplast migrates to the posterior end of the host cell. Thus, in this phase of the cell cycle this Crithidia species is morphologically similar to the proliferative opisthomastigote forms described in the symbiont-harboring trypanosomatid Herpetomonas roitmani [34].…”

Section: Discussionsupporting

confidence: 66%

The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus

et al. 2010

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In trypanosomatids, cell division involves morphological changes and requires coordinated replication and segregation of the nucleus, kinetoplast and flagellum. In endosymbiont-containing trypanosomatids, like Crithidia deanei, this process is more complex, as each daughter cell contains only a single symbiotic bacterium, indicating that the prokaryote must replicate synchronically with the host protozoan. In this study, we used light and electron microscopy combined with three-dimensional reconstruction approaches to observe the endosymbiont shape and division during C. deanei cell cycle. We found that the bacterium replicates before the basal body and kinetoplast segregations and that the nucleus is the last organelle to divide, before cytokinesis. In addition, the endosymbiont is usually found close to the host cell nucleus, presenting different shapes during the protozoan cell cycle. Considering that the endosymbiosis in trypanosomatids is a mutualistic relationship, which resembles organelle acquisition during evolution, these findings establish an excellent model for the understanding of mechanisms related with the establishment of organelles in eukaryotic cells.

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Section: Discussionsupporting

confidence: 66%

The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus

et al. 2010

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“…Crithidia deanei and H. roitmani present a bacterium‐like endosymbiont in their cytoplasm (Faria‐e‐Silva et al 1996; Rozental et al 1987). The symbionts of C. deanei can influence the phagocytosis of these parasites by macrophages, although no further details about their intracellular cycle have been presented (Rozental et al 1987).…”

Section: Discussionmentioning

confidence: 99%

Infection of Mouse Dermal Fibroblasts by the Monoxenous Trypanosomatid Protozoa Crithidia deanei and Herpetomonas roitmani

Santos

Bourguignon

Castro

et al. 2004

J Eukaryotic Microbiology

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Traditionally, monoxenous trypanosomatid protozoa are not believed to infect vertebrate cells. Using light and electron microscopy, we show that the monoxenous trypanosomatids Crithidia deanei and Herpetomonas roitmani are able to infect dermal mouse fibroblasts in vitro. We present experimental evidence of phagocytosis of these trypanosomatids, and demonstrate their survival in vertebrate cells. This paper raises the question about the role of C. deanei and H. roitmani, and perhaps other monoxenous trypanosomatid species, in opportunistic infections of immunocompromised individuals and cutaneos lesions in vertebrate hosts.

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“…This parasite was isolated as opisthomorph forms, which proliferate in culture unlike other Herpetomonas species (Faria-e-Silva et al 1996). Promastigote and opisthomorph forms of H. roitmani display a net negative charge of about −0.73 and −1.20µm.s −1 .V −1 .cm, respectively.…”

Section: Cellular Electrophoretic Mobilitymentioning

confidence: 99%

The surface charge of trypanosomatids

Souto-Padrón

2002

An. Acad. Bras. Ciênc.

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The surface charge of trypanosomatids was evaluated by means of the binding of cationic particles, as visualized by electron microscopy and by direct measurements of the electrophoretic mobility of cells. The results obtained indicate that most of the trypanosomatids exhibit a negatively charged surface whose value is species specific and varies according to the developmental stages. Sialic acids associated with glycoproteins, glycolipids and phosphate groups are the major components responsible for the net negative surface charge of the trypanosomatids.

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Proliferative opisthomastigote forms in Herpetomonas roitmani (Kinetoplastida: Trypanosomatidae)

Cited by 9 publications

References 20 publications

The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus

The Bacterium Endosymbiont of Crithidia deanei Undergoes Coordinated Division with the Host Cell Nucleus

Infection of Mouse Dermal Fibroblasts by the Monoxenous Trypanosomatid Protozoa Crithidia deanei and Herpetomonas roitmani

The surface charge of trypanosomatids

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