Proliferative behaviour and cytogenetic changes in human renal-cell carcinoma

Riese, Werner de; Allhoff, E. P.; Werner, Martin; Atzpodien, Jens; Kirchner, Hartmut; Stief, Christian G.; Djamilian, Mohamad H.; Schlick, R. W.; Jonas, Udo

doi:10.1007/bf00184038

Cited by 13 publications

(18 citation statements)

References 18 publications

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“…As recently described, growth fractions of cultur ed tumor cells can also be immunocytochemically determined on special slide chambers [15,31]. Ki-67 staining is delineated schematically in figure 2: The acetone-and chloroform-fixed cells (in vivo: 5p frozen sec tions; in vitro: cells are directly attached on the slides [15]) arc exposed for approximately 30 minutes to the monoclonal antibody (Ki-67) which has been diluted in RPMI 1640 me dium. After washing in TRIS-buffered solution (pH 7.4 -7.6), the cells are incubated with rabbit-anti-mouse IgG and then exposed to APAAP (alkaline phosphatase anti-alkaline pho sphatase) complex after repeated washing [26].…”

Section: Ki-67 Stainingmentioning

confidence: 99%

“…Due to the expense this equip ment is only available in research and medical centers. Al though it is a valid and reliable parameter in RCC, DNAcytometry did not prove successful in routine diagnostics [15].…”

Section: Dna-cytometry For Rccmentioning

confidence: 99%

“…3,4). Different human malignancies have already been investigated using the Ki-67 immunohistochemistry [1,2,15,27,29,30,32,33,34,35]: The in vivo proliferation rate of RCC is low, only ranging up to 25% [15,36]. Because of this low proliferative potential, only a few mitotic cells can be obtained by biopsy.…”

Section: Ki-67 Stainingmentioning

confidence: 99%

“…Ki-67 staining is performed as follows: Tissue biopsies must be stored in fluid nitrogen to get 5p frozen sections for the immunohistochemical staining; formalin fixed samples are not suitable [25]. As recently described, growth fractions of cultur ed tumor cells can also be immunocytochemically determined on special slide chambers [15,31]. Ki-67 staining is delineated schematically in figure 2: The acetone-and chloroform-fixed cells (in vivo: 5p frozen sec tions; in vitro: cells are directly attached on the slides [15]) arc exposed for approximately 30 minutes to the monoclonal antibody (Ki-67) which has been diluted in RPMI 1640 me dium.…”

Section: Ki-67 Stainingmentioning

confidence: 99%

“…During short-term in vitro culturing, the proliferation rates of malignant renal cells increase up to 85%. This phenomenon improves the cytogenetical output [15]. Malignant renal cells prepared in vitro in the manner as recently described were characterized by employing conventional cytological, cytochemical and immunocytochemical measures [40]: Malignant re nal cells were exclusively obtained by the cell-cluster prepara tion technique [7].…”

Section: Ki-67 Stainingmentioning

confidence: 99%

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Proliferation Kinetics and Prognosis of Renal Cell Carcinoma

Riese¹,

Allhoff²,

Werner³

et al. 1991

Oncol Res Treat

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The historical development of different techniques for the study of proliferative patterns of normal and malignant tissue is explained. For more than 100 years, pathologists and clinicians have attempted to clarify the prognostic impact of this tumor-biological parameter. One relevant result of these investigations is the ‘Grading-System’, a histomorphological description of tumor cell differentiation. The auto-radiographic method using tritiated thymidine (thymidine incorporation assay) detects a portion of DNA-sythesizing cells directly. However, radioactivity and long autoradiographic exposure times are the main disadvantages. DNA cytophotometry and immunohistochemical labeling are currently the most frequently used techniques for direct assessment of proliferating cells in normal and malignant renal tissue as well as in other human tumor tissues. Concerning renal cell carcinoma (RCC), our own experience and the results of the literature are compared and discussed: In contrast to tumor staging, tumor grading correlates with the tumor-specific proliferation rates. According to the present data, the measurement of the proliferative kinetics seems to be a relevant prognostic parameter for detection of high-risk patients, especially in the early stages showing identical histological findings for staging and grading.

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Section: Ki-67 Stainingmentioning

confidence: 99%

Section: Dna-cytometry For Rccmentioning

confidence: 99%

Section: Ki-67 Stainingmentioning

confidence: 99%

Section: Ki-67 Stainingmentioning

confidence: 99%

Section: Ki-67 Stainingmentioning

confidence: 99%

See 3 more Smart Citations

Proliferation Kinetics and Prognosis of Renal Cell Carcinoma

Riese¹,

Allhoff²,

Werner³

et al. 1991

Oncol Res Treat

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Flow‐cytometric and quantitative histologic parameters as prognostic indicators for occult retroperitoneal disease in clinical‐stage‐I non‐seminomatous testicular germ‐cell tumors

Riese

Ulbright

et al. 1994

Intl Journal of Cancer

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Our study was performed to clarify whether the combination of DNA flow-cytometric and quantitative histopathological parameters improves the prediction of occult metastatic disease in clinical stage-I non-seminomatous testicular germ-cell tumors (NSGCT). We used archival paraffin primary-tumor tissue of 67 clinical stage-I NSGCT patients who had undergone retroperitoneal lymph-node dissection (RPLND). According to the RPLND specimens, 24 patients were at pathological stage I and 43 at pathological stage II. Archival blocks were redissected for histological re-evaluation. In addition, 50 microns sections were prepared according to the Hedley technique in order to obtain nuclear suspensions which were processed for flow cytometry (FC). In univariate analysis, the percentage of embryonal carcinoma, the percentage of immature teratoma and vascular invasion were the most accurate predictive histopathological parameters. The percentage of aneuploid cells in S-phase was the best predictive FC parameter. In multivariate analysis, the percentage of embryonal carcinoma and the S-phase fraction of aneuploid cells were the only independent markers for occult metastatic disease. According to this statistical approach, 91.0% of pathological stage-I and stage-II cases were correctly classified. Sensitivity was 95.3% and specificity was 83.3%. Using histopathological criteria alone, only 56.7% NSGCT patients were correctly classified.

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