Proliferative and Nonproliferative Lesions of the Rat and Mouse Central and Peripheral Nervous Systems: New and Revised INHAND Terms

Abstract

Harmonization of diagnostic terminology used during the histopathologic analysis of rodent tissue sections from nonclinical toxicity studies will improve the consistency of data sets produced by laboratories located around the world. The INHAND Project ( International Harmonization of Nomenclature and Diagnostic Criteria for Lesions in Rats and Mice) is a cooperative enterprise of 4 major societies of toxicologic pathology to develop a globally accepted standard vocabulary for proliferative and nonproliferative lesions in rodents. A prior manuscript ( Toxicol Pathol 2012;40[4 Suppl]:87S-157S) defined multiple diagnostic terms for toxicant-induced lesions, common spontaneous and age-related changes, and principal confounding artifacts in the rat and mouse central nervous system (CNS) and peripheral nervous system (PNS). The current article defines 9 new diagnostic terms and updates 2 previous terms for findings in the rodent CNS and PNS, the need for which has become evident in the years since the publication of the initial INHAND nomenclature for findings in rodent neural tissues. The nomenclature presented in this document is also available electronically on the Internet at the goRENI website ( http://www.goreni.org/ ).

CAR T cell manufacturing from naive/stem memory T lymphocytes enhances antitumor responses while curtailing cytokine release syndrome

“…With the aim of evaluating signs of neurotoxicity concomitant with CRS development, mouse brains were collected at sacrifice and subjected to histopathological evaluation. Impressively, 3 out of the 5 CAR T BULK –treated mice presented multifocal brain hemorrhages ( 42 ), whereas in the group infused with CAR T N/SCM only 1 mouse showed a small hemorrhagic focus ( Supplemental Figure 4C ).…”

Pectic Galactan Polysaccharide‐Based Gene Delivery System for Targeting Neuroinflammation

“…The morphological characteristics of the different regions of the lesion were in agreement with the state-of-the-art harmonized terminology used for histopathologic analysis of rodent tissue sections. [75] From this evaluation it was evident that the targeted region, the gliosis layer, in which Gal-3 overexpressing cells are found, [27][28][29][30][31][32] was clearly observed seven days after the injury assay. This characterization of the injury and the clear gliosis layer observed, shows that this inflammatory assay in an in vivo rodent model is a reliable model for examining the ability of the designed complexes to selectively transfect reactive gliosis in neuroinflammation.…”

“…Sections were than stained with hematoxylin/eosin (H&E) and brain tissue was examined by histopathological evaluation, which was done in a blinded manner using a state-of-the-art harmonized terminology of rodent tissue sections from nonclinical toxicity studies. [75] Complex Administration: After surgical anesthesia was confirmed, rats (6 per group) were placed in a stereotactic frame and secured with teeth and ear bars. A 25-gauge needle was inserted at the center of the drilled hole to a constant depth (inserted to a depth of 2.5 mm and then retracted 0.5 mm) reaching the injured area in the cortex.…”

International Harmonization of Nomenclature and Diagnostic Criteria (INHAND): Nonproliferative and Proliferative Lesions of the Minipig

“…For detailed general considerations on the nervous system, please refer to the INHAND publication on rodent nervous system. 120,121 The cellular composition of minipig nervous system is comparable to that of other mammals.…”