Proliferative activity of rat hepatocytes transplanted into the spleen

Kokudo, Norihiro

doi:10.1016/0963-6897(94)00066-s

Cited by 1 publication

(4 citation statements)

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“…There have been several reports on the functionality of hepatocytes after transplantation in Gunn rats (Kokudo et al 1995a;Cubero et al 2001). Coincident to the results obtained by Kokudo et al (1995b) the improvement in serum hyperbilirubinaemia was not statistically different between foetal and adult hepatocyte transplantation. Furthermore, after FHT the decrease in TSB is more remarkable from 3 months on after transplantation (Kokudo et al 1995a).…”

Section: Discussionsupporting

confidence: 71%

“…However, percentage of BCB was higher in the RS + T 3 injected animals transplanted with foetal hepatocytes. Our findings are coincidental but slightly greater than Kokudo's et al (1995b) who observed considerable amounts of bilirubin glucuronides in the bile from Gunn rats 4 months after FHT (36.0%) and AHT (27.0%). We have reported glucuronidation percentages around 66% after FHT in our preliminary report .…”

Section: Discussionsupporting

confidence: 52%

“…In this study we have found BCB around 70-80% after either FHT or AHT in both the RS-pre-treated and the RS + T 3 groups. This can be explained by the greater number of cells that we transplant, 40 × 10 6 in contrast to Kokudo et al (1995b) who only transplanted 10 × 10 6 . Recently, RS pre-treatment alone has been demonstrated to induce repopulation of a liver by transplanted cells (Laconi et al 2001).…”

Section: Discussionmentioning

confidence: 88%

“…The immunohistochemical detection of PCNA has been found to depend on the proliferative potential of a number of cell types including hepatocytes (Kokudo et al 1995b). We therefore applied this method to estimate hepatocyte proliferation in the liver.…”

Section: Discussionmentioning

confidence: 99%

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Hepatic proliferation in Gunn rats transplanted with hepatocytes: effect of retrorsine and tri‐iodothyronine

et al. 2005

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Hepatocyte transplantation would offer an attractive alternative to liver transplantation in the treatment of inborn errors of liver metabolism. However, a major problem in most transplantation studies to date has been the limited growth of transplanted cells in the recipient organ. We performed a strategy for selective proliferation of transplanted cells by interfering with the proliferative capacity of resident hepatocytes, using the pyrrolizidine alkaloid retrorsine and then transplanting liver cells in conjunction with repeated administration of triiodothyronine, an inducer of hepatocyte proliferation in rats. In the present study, foetal and adult syngeneic hepatocyte transplantation into spleen was performed in retrorsine-treated hyperbilirubinemic Gunn rats. In parallel, repeated injections of triiodothyronine were given to recipients. Rats were sacrificed at 1, 7, 30 and 90 days after transplantation and blood and bile samples were taken to assess the functionality of transplanted cells. The proliferative activity of transplanted hepatocytes was evaluated using proliferating cell nuclear antigen labelling index. In summary, both adult and foetal hepatocyte transplantation were effective in correcting a metabolic abnormality in Gunn rats for as long as 3 months. The RS/T3 model, as a measure to increase graft function, could represent an important advance to future clinical application of hepatocyte transplantation.

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Section: Discussionsupporting

confidence: 71%

Section: Discussionsupporting

confidence: 52%