Proliferative Activity in Ischemia/Reperfusion Injury in Hepatectomized Mice: Effect of N-Acetylcysteine

Silva, S.M.; Carbonel, Adriana Aparecida Ferraz; Taha, Murched Omar; Simões, Manuel; Montero, Efs

doi:10.1016/j.transproceed.2012.07.009

Cited by 12 publications

(13 citation statements)

References 28 publications

(6 reference statements)

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“…This prompted us to determine whether oxidative stress could play a role in nuclear inclusion formation. N-acetyl cysteine is an antioxidant used to reduce oxidative stress both experimentally and clinically during acute liver injury [32]. When 9–10 month old HBsAg-Tg mice were treated with NAC, we observed a marked reduction in 4-HNE adduct formation ( Fig.…”

Section: Resultsmentioning

confidence: 78%

Dysplastic Hepatocytes Develop Nuclear Inclusions in a Mouse Model of Viral Hepatitis

et al. 2014

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Viral hepatitis resulting in chronic liver disease is an important clinical challenge and insight into the cellular processes that drive pathogenesis will be critical in order to develop new diagnostic and therapeutic options. Nuclear inclusions in viral and non-viral hepatitis are well documented and have diagnostic significance in some disease contexts. However, the origins and functional consequences of these nuclear inclusions remain elusive. To date the clinical observation of nuclear inclusions in viral and non-viral hepatitis has not been explored at depth in murine models of liver disease. Herein, we report that in a transgenic model of hepatitis B surface antigen mediated hepatitis, murine hepatocytes exhibit nuclear inclusions. Cells bearing nuclear inclusions were more likely to express markers of cell proliferation. We also established a correlation between these inclusions and oxidative stress. N-acetyl cysteine treatment effectively reduced oxidative stress levels, relieved endoplasmic reticulum (ER) stress, and the number of nuclear inclusions we observed in the transgenic mice. Our results suggest that the presence of nuclear inclusions in hepatocytes correlates with oxidative stress and cellular proliferation in a model of antigen mediated hepatitis.

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Section: Resultsmentioning

confidence: 78%

Dysplastic Hepatocytes Develop Nuclear Inclusions in a Mouse Model of Viral Hepatitis

et al. 2014

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“…Furthermore, laboratory data have shown that NAC reduces the level of hepatic ROS and attenuate ROS‐induced liver injury after IRI . In addition to a reduction in lipid peroxidation, NAC also enhances regeneration of the liver and increases glutathione levels . Although tolerated well, there is still 3–6% anaphylactic reaction rate with intravenous use, that tends to resolve with either slowing or cessation of the infusion …”

Section: Discussionmentioning

confidence: 99%

“…Due to the results observed in liver transplantation studies, NAC has been subsequently used in patients undergoing major liver resection to prevent PHLF. Experimental studies have reported promising results on liver regeneration following the administration of NAC, and it is thought its mechanism of action is related to decreasing oxidative stress and the effects of IRI, in particular, in rats with a background of liver steatosis . Nevertheless, there is a lack of clinical data to support the use of NAC following liver resection.…”

Section: Introductionmentioning

confidence: 99%

Current evidence for the use of N‐acetylcysteine following liver resection

Kemp

Mole

Gomez

2017

ANZ Journal of Surgery

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“…One of the proposals found in the literature to lower or abolish the effects of oxidative stress is the exogenous addition of glutathione or its precursor, n-acetylcysteine 22,23 . A higher dose of such substances, even if controversial, may be beneficial because as the dose and duration of the treatment seems to be of value for reducing the damage involved in the ischemic tissue.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

Ikejiri

Neto²,

Chaves³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion. METHODS:Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm [2^(ΔΔCt)], were considered to be biologically meaningful. RESULTS:Out of a total of 84 genes in the lung that are related to oxidative stress, 67 (79.7%) were up-regulated and 17 (20.2%) were down-regulated. Only two genes (2.3%), Lpo (lactoperoxidase) (+3.51) and Gpx4 (glutathione peroxidase) (+4.10), were expressed above the three-fold threshold, while none of the down-regulated genes were expressed outside of this threshold. CONCLUSION:The intestinal ischemia/reperfusion injury promoted a gene expression profile consisting of the positive expression of oxidative genes in a remote organ. This suggests that activate signaling pathways are implicated in both cell survival and the maintenance of genome integrity in the lung.Key words: Gene Expression. Oxidative Stress. Lung. Reperfusion Injury. Mice. Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

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Proliferative Activity in Ischemia/Reperfusion Injury in Hepatectomized Mice: Effect of N-Acetylcysteine

Abstract: NAC preserved the remnant liver in mice and stimulates regeneration even after IR injury.

Cited by 12 publications

References 28 publications

Dysplastic Hepatocytes Develop Nuclear Inclusions in a Mouse Model of Viral Hepatitis

Dysplastic Hepatocytes Develop Nuclear Inclusions in a Mouse Model of Viral Hepatitis

Current evidence for the use of N‐acetylcysteine following liver resection

Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

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