Proliferation, survival and cell death in fish gills remodeling: From injury to recovery

Sales, Camila Ferreira; Santos, Keiza Priscila Enes dos; Rizzo, Elizete; Ribeiro, Rosy Iara Maciel de Azambuja; Santos, Hélio Batista dos

doi:10.1016/j.fsi.2017.07.001

Cited by 41 publications

(42 citation statements)

References 53 publications

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“…Petechiae that are minor in size and extent, as seen in controls, are reversible changes [72,80]. Lamellar aneurysms and complete lamellar fusions are severe pathologies [72,81]. Lamellar aneurysms result in damage and loss of pillar cells in these areas result in the fusion of capillaries within secondary lamellae, which causes their dilation and congestion with blood [75].…”

Section: Gills and Branchial Chambermentioning

confidence: 99%

“…We considered the possibility of recovery from these phenotypic traits should fish be moved to clean water. Recovery of aneurysms is somewhat controversial [81]. Severe changes such as these are often irreversible even when water quality improves [72,80].…”

Section: Gills and Branchial Chambermentioning

confidence: 99%

“…For example, Hypostomus francisci (a Brazillian catfish sp.) collected from a polluted river exhibited epithelial hypertrophy and lifting, lamellar fusion, aneurysms, hyperemia, and vascular congestion [81]. While recovery was slow after placement in clean water, full recovery of lamellar aneurysms occurred after 30 days and apoptosis was stimulated to promote gill structure recovery [81].…”

Section: Gills and Branchial Chambermentioning

confidence: 99%

“…collected from a polluted river exhibited epithelial hypertrophy and lifting, lamellar fusion, aneurysms, hyperemia, and vascular congestion [81]. While recovery was slow after placement in clean water, full recovery of lamellar aneurysms occurred after 30 days and apoptosis was stimulated to promote gill structure recovery [81]. In a laboratory study, aneurysms developed on tips of primary lamellae of Prochilodus scrofa (a tropical teleost fish) exposed to copper for 96 hrs, with additional damage in the form of epithelial lifting, cell swelling, and proliferation of pavement, chloride, and mucous cells [86].…”

Section: Gills and Branchial Chambermentioning

confidence: 99%

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Chronic microfiber exposure in adult Japanese medaka (Oryzias latipes)

Chernick

Lewis

et al. 2020

PLoS ONE

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Microplastic fibers (MFs) pollute aquatic habitats globally via sewage release, stormwater runoff, or atmospheric deposition. Of the synthetic MFs, polyester (PES) and polypropylene (PP) are the most common. Field studies show that fish ingest large quantities of MFs. However, few laboratory studies have addressed host responses, particularly at the organ and tissue levels. Adult Japanese medaka (Oryzias latipes), a laboratory model fish, were exposed to aqueous concentrations of PES or PP MFs (10,000 MFs/L) for 21 days. Medaka egested 1,367 ± 819 PES MFs (0.1 ± 0.04 mg) and 157 ± 105 PP MFs (1.4 ± 0.06 mg) per 24 hrs, with PP egestion increasing over time. Exposure did not result in changes in body condition, gonadosomatic-or hepatosomatic indices. PES exposure resulted in no reproductive changes, but females exposed to PP MFs produced more eggs over time. MF exposure did not affect embryonic mortality, development, or hatching. Scanning electron microscopy (SEM) of gills revealed denuding of epithelium on arches, fusion of primary lamellae, and increased mucus. Histologic sections revealed aneurysms in secondary lamellae, epithelial lifting, and swellings of inner opercular membrane that altered morphology of rostral most gill lamellae. SEM and histochemical analyses showed increased mucous cells and secretions on epithelium of foregut; however, overt abrasions with sloughing of cells were absent. For these reasons, increased focus at the tissue and cell levels proved necessary to appreciate toxicity associated with MFs. PLOS ONEPLOS ONE | https://doi.org/10.

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Section: Gills and Branchial Chambermentioning

confidence: 99%

Section: Gills and Branchial Chambermentioning

confidence: 99%

Section: Gills and Branchial Chambermentioning

confidence: 99%

Section: Gills and Branchial Chambermentioning

confidence: 99%

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Chronic microfiber exposure in adult Japanese medaka (Oryzias latipes)

Chernick

Lewis

et al. 2020

PLoS ONE

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“…Results of the in vivo experiment supported the in vivo data. PCNA was used as a biomarker to analyze cell viability in prostatic tissues ( 31 , 32 ). The results demonstrated that MRE11 expression was decreased whereas PCNA was increased.…”

Section: Discussionmentioning

confidence: 99%

High glucose induces the proliferation of prostatic cells via downregulating MRE11

Cai

et al. 2018

Int J Mol Med

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The aim of the present study was to investigate the candidate genes and pathways associated with benign prostatic hyperplasia (BPH) and diabetes. In vitro experiments were performed using normal prostatic epithelial RWPE-1 and HPr-1 cells. The cell lines were treated with a high-glucose solution and MTS and bromodeoxyuridine assays were used to assess cell viability. Transcriptome sequencing was used to screen the candidate genes. The expression of candidate genes was further verified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. A meiotic recombination 11 (MRE11) overexpression vector was designed and transfected into RWPE-1 cells to verify the function of MRE11. A streptozotocin-induced diabetic rat model was established and rat MRE11 levels were determined by RT-qPCR and immunohistochemical staining. High concentrations of glucose resulted in RWPE-1 and HPr-1 cells with high viability. Transcriptome sequencing revealed that MRE11 was downregulated when RWPE-1 cells were exposed to high-glucose conditions. When MRE11 was overexpressed, cell viability decreased and cell apoptosis was induced under high-glucose conditions. Prostatic tissues from rats were collected and assessed; MRE11 expression was observed to be decreased, which was consistent with the in vitro cell experiments. BPH may be associated with diabetes, as MRE11 expression in prostatic cells was decreased when exposed to high-glucose conditions. Therefore, MRE11 may have potential as a biomarker for the early diagnosis of BPH and diabetes.

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